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Microorganisms
Volume 9
Issue 2
10.3390/microorganisms9020283
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Article

Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA

by
Ivars Petrovskis
,
Ilva Lieknina
,
Andris Dislers
,
Juris Jansons
,
Janis Bogans
,
Inara Akopjana
,
Jelena Zakova
and
Irina Sominskaya
*
Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia
*
Author to whom correspondence should be addressed.
Microorganisms 2021, 9(2), 283; https://doi.org/10.3390/microorganisms9020283
Submission received: 31 December 2020 / Revised: 22 January 2021 / Accepted: 26 January 2021 / Published: 30 January 2021
(This article belongs to the Special Issue Viral Immunogens and Vaccines)
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Abstract

The core proteins (HBc) of the hepatitis B virus (HBV) genotypes A, B, C, D, E, F, and G were cloned and expressed in Escherichia coli (E. coli), and HBc-formed virus-like particles (VLPs) were purified with ammonium sulfate precipitation, gel filtration, and ion exchange chromatography (IEX). The best VLP yield was found for the HBc of the HBV genotypes D and G. For the HBc of the HBV genotypes D, F, and G, the possibility of dissociation and reassociation maintaining the native HBc structure was demonstrated. Single-stranded (ss) and double-stranded (ds) ribonucleic acid (RNA) was successfully packed into HBc VLPs for the HBV genotypes D and G.
Keywords: HBV genotypes; HBcAg expression; encapsidation HBV genotypes; HBcAg expression; encapsidation

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MDPI and ACS Style

Petrovskis, I.; Lieknina, I.; Dislers, A.; Jansons, J.; Bogans, J.; Akopjana, I.; Zakova, J.; Sominskaya, I. Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA. Microorganisms 2021, 9, 283. https://doi.org/10.3390/microorganisms9020283

AMA Style

Petrovskis I, Lieknina I, Dislers A, Jansons J, Bogans J, Akopjana I, Zakova J, Sominskaya I. Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA. Microorganisms. 2021; 9(2):283. https://doi.org/10.3390/microorganisms9020283

Chicago/Turabian Style

Petrovskis, Ivars, Ilva Lieknina, Andris Dislers, Juris Jansons, Janis Bogans, Inara Akopjana, Jelena Zakova, and Irina Sominskaya. 2021. "Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA" Microorganisms 9, no. 2: 283. https://doi.org/10.3390/microorganisms9020283

APA Style

Petrovskis, I., Lieknina, I., Dislers, A., Jansons, J., Bogans, J., Akopjana, I., Zakova, J., & Sominskaya, I. (2021). Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA. Microorganisms, 9(2), 283. https://doi.org/10.3390/microorganisms9020283

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