Next Article in Journal
Characterization of “Candidatus Ehrlichia Pampeana” in Haemaphysalis juxtakochi Ticks and Gray Brocket Deer (Mazama gouazoubira) from Uruguay
Previous Article in Journal
Occurrence of Hybrid Diarrhoeagenic Escherichia coli Associated with Multidrug Resistance in Environmental Water, Johannesburg, South Africa
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microorganisms
Volume 9
Issue 10
10.3390/microorganisms9102164
microorganisms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Evidence of HIV-1 Genital Shedding after One Year of Antiretroviral Therapy in Females Recently Diagnosed in Bamako, Mali

by
Abdelaye Keita
1,2,
Josselin Rigaill
2,3,
Sylvie Pillet
2,3,
Youssouf Sereme
2,
Souleymane Coulibaly
1,
Fodé Diallo
4,
Paul Verhoeven
2,3,
Bruno Pozzetto
2,3,
Tenin Aoua Thiero
1 and
Thomas Bourlet
2,3,*
1
Département Qualité Sécurité et Sûreté Biologique, Institut National de Recherche en Santé Publique (INRSP), Bamako BP 1771, Mali
2
GIMAP Team 15, Inserm, U1111, CNRS, UMR5308, Centre International de Recherche en Infectiologie, Faculty of Medicine, University of Lyon 1, 42000 Saint-Etienne, France
3
Laboratoire des Agents Infectieux et d’Hygiène, Biology Pathology Department, University Hospital of Saint-Etienne, 42000 Saint-Etienne, France
4
Centre d’Ecoute de Soins et d’Accompagnement (CESAC), ARCAD/SIDA Clinic, Bamako BPE 2561, Mali
*
Author to whom correspondence should be addressed.
Microorganisms 2021, 9(10), 2164; https://doi.org/10.3390/microorganisms9102164
Submission received: 7 September 2021 / Revised: 6 October 2021 / Accepted: 12 October 2021 / Published: 17 October 2021
(This article belongs to the Special Issue Management and Antiretroviral Treatment of HIV Infection)
Browse Figures
Versions Notes

Abstract

Little is known about the dynamic of HIV-1 shedding and resistance profiles in the female genital reservoir after antiretroviral therapy (ART) initiation in resource-limited countries (RLCs), which is critical for evaluating the residual sexual HIV-1 transmission risk. The present study aimed to evaluate the efficacy of 1 year duration ART at blood and genital levels in females newly diagnosed for HIV-1 from three centers in Bamako, Mali. Seventy-eight consenting females were enrolled at the time of their HIV-1 infection diagnosis. HIV-1 RNA loads (Abbott Real-Time HIV-1 assay) were tested in blood and cervicovaginal fluids (CVF) before and 12 months after ART initiation. Primary and acquired resistances to ART were evaluated by ViroseqTM HIV-1 genotyping assay. The vaginal microbiota was analyzed using IonTorrentTM NGS technology (Thermo Fisher Scientific). Proportions of primary drug resistance mutations in blood and CVF were 13.4% and 25%, respectively. Discrepant profiles were observed in 25% of paired blood/CVF samples. The acquired resistance rate was 3.1% in blood. At month 12, undetectable HIV-1 RNA load was reached in 84.6% and 75% of blood and CVF samples, respectively. A vaginal dysbiosis was associated with HIV RNA shedding. Our findings emphasize the need of reinforcing education to improve retention in care system, as well as the necessity of regular virological monitoring before and during ART and of implementing vaginal dysbiosis diagnosis and treatment in RLCs.
Keywords: HIV-1; antiretroviral therapy; genital reservoir; resistance; microbiota; Mali HIV-1; antiretroviral therapy; genital reservoir; resistance; microbiota; Mali

Share and Cite

MDPI and ACS Style

Keita, A.; Rigaill, J.; Pillet, S.; Sereme, Y.; Coulibaly, S.; Diallo, F.; Verhoeven, P.; Pozzetto, B.; Thiero, T.A.; Bourlet, T. Evidence of HIV-1 Genital Shedding after One Year of Antiretroviral Therapy in Females Recently Diagnosed in Bamako, Mali. Microorganisms 2021, 9, 2164. https://doi.org/10.3390/microorganisms9102164

AMA Style

Keita A, Rigaill J, Pillet S, Sereme Y, Coulibaly S, Diallo F, Verhoeven P, Pozzetto B, Thiero TA, Bourlet T. Evidence of HIV-1 Genital Shedding after One Year of Antiretroviral Therapy in Females Recently Diagnosed in Bamako, Mali. Microorganisms. 2021; 9(10):2164. https://doi.org/10.3390/microorganisms9102164

Chicago/Turabian Style

Keita, Abdelaye, Josselin Rigaill, Sylvie Pillet, Youssouf Sereme, Souleymane Coulibaly, Fodé Diallo, Paul Verhoeven, Bruno Pozzetto, Tenin Aoua Thiero, and Thomas Bourlet. 2021. "Evidence of HIV-1 Genital Shedding after One Year of Antiretroviral Therapy in Females Recently Diagnosed in Bamako, Mali" Microorganisms 9, no. 10: 2164. https://doi.org/10.3390/microorganisms9102164

APA Style

Keita, A., Rigaill, J., Pillet, S., Sereme, Y., Coulibaly, S., Diallo, F., Verhoeven, P., Pozzetto, B., Thiero, T. A., & Bourlet, T. (2021). Evidence of HIV-1 Genital Shedding after One Year of Antiretroviral Therapy in Females Recently Diagnosed in Bamako, Mali. Microorganisms, 9(10), 2164. https://doi.org/10.3390/microorganisms9102164

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop