Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation

Slaný, Ondrej; Klempová, Tatiana; Shapaval, Volha; Zimmermann, Boris; Kohler, Achim; Čertík, Milan

doi:10.3390/microorganisms9010170

Open AccessArticle

Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation

by

Ondrej Slaný

^1,*

,

Tatiana Klempová

¹

,

Volha Shapaval

²,

Boris Zimmermann

²

,

Achim Kohler

² and

Milan Čertík

¹

Faculty of Chemical and Food Technology, Institute of Biotechnology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia

²

Faculty of Science and Technology, Norwegian University of Life Sciences, Postbox 5003, 1432 Ås, Norway

^*

Author to whom correspondence should be addressed.

Microorganisms 2021, 9(1), 170; https://doi.org/10.3390/microorganisms9010170

Submission received: 1 December 2020 / Revised: 7 January 2021 / Accepted: 11 January 2021 / Published: 14 January 2021

(This article belongs to the Special Issue Microbial Populations of Fermented Foods)

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Abstract

The method of solid-state fermentation (SSF) represents a powerful technology for the fortification of animal-based by-products. Oleaginous Zygomycetes fungi are efficient microbial cell factories used in SSF to valorize a wide range of waste and rest cereal materials. The application of this fermentation technique for utilization and biotransformation of animal-based materials represents a distinguished step in their treatment. In this study, for the first time, the strain Umbelopsis isabellina CCF2412 was used for the bioconversion of animal fat by-products to the fermented bioproducts enriched with n-6 polyunsaturated fatty acids, mainly γ-linolenic acid (GLA). Bioconversion of both cereals and the animal fat by-product resulted in the production of fermented bioproducts enriched with not just GLA (maximal yield was 6.4 mg GLA/g of fermented bioproduct), but also with high yields of glucosamine. Moreover, the fermentation on the cornmeal matrix led to obtaining bioproduct enriched with β-carotene. An increased amount of β-carotene content improved the antioxidant stability of obtained fermented bioproducts. Furthermore, the application of Fourier-transform infrared spectroscopy for rapid analysis and characterization of the biochemical profile of obtained SSF bioproducts was also studied.

Keywords: solid-state fermentation; Umbelopsis isabellina; γ-linolenic acid; β-carotene; Fourier transform infrared (FTIR) spectroscopy; animal fat by-product

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MDPI and ACS Style

Slaný, O.; Klempová, T.; Shapaval, V.; Zimmermann, B.; Kohler, A.; Čertík, M. Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation. Microorganisms 2021, 9, 170. https://doi.org/10.3390/microorganisms9010170

AMA Style

Slaný O, Klempová T, Shapaval V, Zimmermann B, Kohler A, Čertík M. Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation. Microorganisms. 2021; 9(1):170. https://doi.org/10.3390/microorganisms9010170

Chicago/Turabian Style

Slaný, Ondrej, Tatiana Klempová, Volha Shapaval, Boris Zimmermann, Achim Kohler, and Milan Čertík. 2021. "Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation" Microorganisms 9, no. 1: 170. https://doi.org/10.3390/microorganisms9010170

APA Style

Slaný, O., Klempová, T., Shapaval, V., Zimmermann, B., Kohler, A., & Čertík, M. (2021). Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation. Microorganisms, 9(1), 170. https://doi.org/10.3390/microorganisms9010170

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Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation

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