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Open AccessArticle

Cutibacterium acnes Biofilm Study during Bone Cells Interaction

1
EA 4691 Biomatériaux et Inflammation en Site Osseux (BIOS), Université de Reims Champagne-Ardenne, SFR Cap Santé (FED 4231), 51100 Reims, France
2
Service Pharmacie, Centre Hospitalier Universitaire de Reims (CHU Reims), 51100 Reims, France
3
Department of Microbiology and Immunology, Faculty of Pharmacy, Helwan University, Cairo 11795, Egypt
4
Department of Microbiology, Faculty of Pharmacy, Ahram Canadian University, Cairo 12585, Egypt
5
Laboratoire de Bactériologie-Hygiène, Centre Hospitalier Universitaire de Reims (CHU Reims), 51100 Reims, France
6
Service d’Orthopédie et Traumatologie, Centre Hospitalier Universitaire de Reims (CHU Reims), 51100 Reims, France
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(9), 1409; https://doi.org/10.3390/microorganisms8091409
Received: 30 July 2020 / Revised: 31 August 2020 / Accepted: 10 September 2020 / Published: 12 September 2020
(This article belongs to the Special Issue Biofilm Implant Related Infections)
Cutibacterium acnes is an opportunistic pathogen involved in Bone and Prosthesis Infections (BPIs). In this study, we observed the behavior of commensal and BPI C. acnes strains in the bone environment through bacterial internalization by osteoblast-like cells and biofilm formation. For the commensal strains, less than 1% of the bacteria were internalized; among them, about 32.7 ± 3.9% persisted intracellularly for up to 48 h. C. acnes infection seems to have no cytotoxic effect on bone cells as detected by LDH assay. Interestingly, commensal C. acnes showed a significant increase in biofilm formation after osteoblast-like internalization for 50% of the strains (2.8-fold increase). This phenomenon is exacerbated on a titanium support, a material used for medical devices. For the BPI clinical strains, we did not notice any increase in biofilm formation after internalization despite a similar internalization rate by the osteoblast-like cells. Furthermore, fluorescent staining revealed more live bacteria within the biofilm after osteoblast-like cell interaction, for all strains (BPIs and commensal). The genomic study did not reveal any link between their clinical origin and phylotype. In conclusion, we have shown for the first time the possible influence of internalization by osteoblast-like cells on commensal C. acnes. View Full-Text
Keywords: joint infections; biofilms; host–pathogen interactions; Cutibacterium acnes; internalization joint infections; biofilms; host–pathogen interactions; Cutibacterium acnes; internalization
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Mongaret, C.; Varin-Simon, J.; Lamret, F.; El-Mahdy, T.S.; Brasme, L.; Vernet-Garnier, V.; Gangloff, S.C.; Ohl, X.; Reffuveille, F. Cutibacterium acnes Biofilm Study during Bone Cells Interaction. Microorganisms 2020, 8, 1409.

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