Next Article in Journal
A Review of Potential Impacts of Climate Change on Coffee Cultivation and Mycotoxigenic Fungi
Previous Article in Journal
Effect of a Debaryomyces hansenii and Lactobacillus buchneri Starter Culture on Aspergillus westerdijkiae Ochratoxin A Production and Growth during the Manufacture of Short Seasoned Dry-Cured Ham
Previous Article in Special Issue
Cutibacterium acnes Biofilm Study during Bone Cells Interaction
Open AccessArticle

Non-Invasive Luciferase Imaging of Type I Interferon Induction in a Transgenic Mouse Model of Biomaterial Associated Bacterial Infections: Microbial Specificity and Inter-Bacterial Species Interactions

1
Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany
2
Lower Saxony Centre for Biomedical Engineering, Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany
3
Institute of Laboratory Animal Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany
4
Department of Orthopedic Surgery, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany
5
Department of Chemical Biology, Helmholtz Center for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(10), 1624; https://doi.org/10.3390/microorganisms8101624
Received: 6 August 2020 / Revised: 14 October 2020 / Accepted: 19 October 2020 / Published: 21 October 2020
(This article belongs to the Special Issue Biofilm Implant Related Infections)
The performance of biomaterials is often compromised by bacterial infections and subsequent inflammation. So far, the conventional analysis of inflammatory processes in vivo involves time-consuming histology and biochemical assays. The present study employed a mouse model where interferon beta (IFN-β) is monitored as a marker for non-invasive rapid detection of inflammation in implant-related infections. The mouse model comprises subcutaneous implantation of morphologically modified titanium, followed by experimental infections with four taxonomically diverse oral bacteria: Streptococcus oralis, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Treponema denticola (as mono culture or selected mixed-culture). IFN-β expression increased upon infections depending on the type of pathogen and was prolonged by the presence of the implant. IFN-β expression kinetics reduced with two mixed species infections when compared with the single species. Histological and confocal microscopy confirmed pathogen-specific infiltration of inflammatory cells at the implant-tissue interface. This was observed mainly in the vicinity of infected implants and was, in contrast to interferon expression, higher in infections with dual species. In summary, this non-invasive mouse model can be used to quantify longitudinally host inflammation in real time and suggests that the polymicrobial character of infection, highly relevant to clinical situations, has complex effects on host immunity. View Full-Text
Keywords: animal model; biomaterial-associated infections; immune response; type I interferon; periodontal pathogens; bioluminescence; non-invasive in vivo imaging animal model; biomaterial-associated infections; immune response; type I interferon; periodontal pathogens; bioluminescence; non-invasive in vivo imaging
Show Figures

Figure 1

MDPI and ACS Style

Rahim, M.I.; Winkel, A.; Lienenklaus, S.; Stumpp, N.S.; Szafrański, S.P.; Kommerein, N.; Willbold, E.; Reifenrath, J.; Mueller, P.P.; Eisenburger, M.; Stiesch, M. Non-Invasive Luciferase Imaging of Type I Interferon Induction in a Transgenic Mouse Model of Biomaterial Associated Bacterial Infections: Microbial Specificity and Inter-Bacterial Species Interactions. Microorganisms 2020, 8, 1624. https://doi.org/10.3390/microorganisms8101624

AMA Style

Rahim MI, Winkel A, Lienenklaus S, Stumpp NS, Szafrański SP, Kommerein N, Willbold E, Reifenrath J, Mueller PP, Eisenburger M, Stiesch M. Non-Invasive Luciferase Imaging of Type I Interferon Induction in a Transgenic Mouse Model of Biomaterial Associated Bacterial Infections: Microbial Specificity and Inter-Bacterial Species Interactions. Microorganisms. 2020; 8(10):1624. https://doi.org/10.3390/microorganisms8101624

Chicago/Turabian Style

Rahim, Muhammad I.; Winkel, Andreas; Lienenklaus, Stefan; Stumpp, Nico S.; Szafrański, Szymon P.; Kommerein, Nadine; Willbold, Elmar; Reifenrath, Janin; Mueller, Peter P.; Eisenburger, Michael; Stiesch, Meike. 2020. "Non-Invasive Luciferase Imaging of Type I Interferon Induction in a Transgenic Mouse Model of Biomaterial Associated Bacterial Infections: Microbial Specificity and Inter-Bacterial Species Interactions" Microorganisms 8, no. 10: 1624. https://doi.org/10.3390/microorganisms8101624

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop