Next Article in Journal
Comparative Genome Analysis of Bacillus sporothermodurans with Its Closest Phylogenetic Neighbor, Bacillus oleronius, and Bacillus cereus and Bacillus subtilis Groups
Next Article in Special Issue
Correction: Moxley, R.A., et al. Intimate Attachment of Escherichia coli O157:H7 to Urinary Bladder Epithelium in the Gnotobiotic Piglet Model. Microorganisms 2020, 8, 263
Previous Article in Journal
Low-Temperature Biosurfactants from Polar Microbes
Previous Article in Special Issue
Is Shiga Toxin-Producing Escherichia coli O45 No Longer a Food Safety Threat? The Danger is Still Out There
Article

Deciphering Additional Roles for the EF-Tu, l-Asparaginase II and OmpT Proteins of Shiga Toxin-Producing Escherichia coli

1
Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile
2
Programa Disciplinario de Inmunología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad De Chile, Santiago 8380453, Chile
3
Instituto Milenio de Inmunología e Inmunoterapia, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile
4
Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción 4030000, Chile
5
Núcleo Milenio Biología de Microbiota Intestinal, Santiago 8320000, Chile
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Current address: Biome Therapeutics, Universidad Mayor, Santiago 8320000, Chile.
Microorganisms 2020, 8(8), 1184; https://doi.org/10.3390/microorganisms8081184
Received: 9 June 2020 / Revised: 31 July 2020 / Accepted: 1 August 2020 / Published: 4 August 2020
(This article belongs to the Special Issue Shiga Toxin-Producing Escherichia coli)
Shiga toxin-producing Escherichia coli (STEC) causes outbreaks and sporadic cases of gastroenteritis. STEC O157:H7 is the most clinically relevant serotype in the world. The major virulence determinants of STEC O157:H7 are the Shiga toxins and the locus of enterocyte effacement. However, several accessory virulence factors, mainly outer membrane proteins (OMPs) that interact with the host cells may contribute to the virulence of this pathogen. Previously, the elongation factor thermo unstable (EF-Tu), l-asparaginase II and OmpT proteins were identified as antigens in OMP extracts of STEC. The known subcellular location of EF-Tu and l-asparaginase II are the cytoplasm and periplasm, respectively. Therefore, we investigate whether these two proteins may localize on the surface of STEC and, if so, what roles they have at this site. On the other hand, the OmpT protein, a well characterized protease, has been described as participating in the adhesion of extraintestinal pathogenic E. coli strains. Thus, we investigate whether OmpT has this role in STEC. Our results show that the EF-Tu and l-asparaginase II are secreted by O157:H7 and may also localize on the surface of this bacterium. EF-Tu was identified in outer membrane vesicles (OMVs), suggesting it as a possible export mechanism for this protein. Notably, we found that l-asparaginase II secreted by O157:H7 inhibits T-lymphocyte proliferation, but the role of EF-Tu at the surface of this bacterium remains to be elucidated. In the case of OmpT, we show its participation in the adhesion of O157:H7 to human epithelial cells. Thus, this study extends the knowledge of the pathogenic mechanisms of STEC. View Full-Text
Keywords: EF-Tu; l-asparaginase II; OmpT; Shiga toxin-producing Escherichia coli; STEC. EF-Tu; l-asparaginase II; OmpT; Shiga toxin-producing Escherichia coli; STEC.
Show Figures

Figure 1

MDPI and ACS Style

Torres, A.N.; Chamorro-Veloso, N.; Costa, P.; Cádiz, L.; Del Canto, F.; Venegas, S.A.; López Nitsche, M.; Coloma-Rivero, R.F.; Montero, D.A.; Vidal, R.M. Deciphering Additional Roles for the EF-Tu, l-Asparaginase II and OmpT Proteins of Shiga Toxin-Producing Escherichia coli. Microorganisms 2020, 8, 1184. https://doi.org/10.3390/microorganisms8081184

AMA Style

Torres AN, Chamorro-Veloso N, Costa P, Cádiz L, Del Canto F, Venegas SA, López Nitsche M, Coloma-Rivero RF, Montero DA, Vidal RM. Deciphering Additional Roles for the EF-Tu, l-Asparaginase II and OmpT Proteins of Shiga Toxin-Producing Escherichia coli. Microorganisms. 2020; 8(8):1184. https://doi.org/10.3390/microorganisms8081184

Chicago/Turabian Style

Torres, Alexia N., Nayaret Chamorro-Veloso, Priscila Costa, Leandro Cádiz, Felipe Del Canto, Sebastián A. Venegas, Mercedes López Nitsche, Roberto F. Coloma-Rivero, David A. Montero, and Roberto M. Vidal 2020. "Deciphering Additional Roles for the EF-Tu, l-Asparaginase II and OmpT Proteins of Shiga Toxin-Producing Escherichia coli" Microorganisms 8, no. 8: 1184. https://doi.org/10.3390/microorganisms8081184

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop