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Article

Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults

1
Division of Food Microbiology and Bioprocesses, Department of Food, Environmental and Nutritional Sciences, University of Milan, 20133 Milan, Italy
2
Division of Human Nutrition, Department of Food, Environmental and Nutritional Sciences, University of Milan, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(4), 492; https://doi.org/10.3390/microorganisms8040492
Received: 12 March 2020 / Revised: 26 March 2020 / Accepted: 27 March 2020 / Published: 30 March 2020
(This article belongs to the Special Issue Probiotics: From Quality Assessment to Microbial Ecology)
The consumption of probiotic products is continually increasing, supported by growing scientific evidence of their efficacy. Considering that probiotics may primarily affect health (either positively or negatively) through gut microbiota modulation, the first aspect that should be evaluated is their impact on the intestinal microbial ecosystem. In this study, we longitudinally analyzed the bacterial taxonomic composition and organic acid levels in four fecal samples collected over the course of four weeks from 19 healthy adults who ingested one capsule a day for two weeks of a formulation containing at least 70 billion colony-forming units, consisting of 25% lactobacilli and 75% Bifidobacterium animalis subsp. lactis. We found that 16S rRNA gene profiling showed that probiotic intake only induced an increase in a single operational taxonomic unit ascribed to B. animalis, plausibly corresponding to the ingested bifidobacterial strain. Furthermore, liquid chromatography/mass spectrometry revealed a significant increase in the lactate and acetate/butyrate ratio and a trend toward a decrease in succinate following probiotic administration. The presented results indicate that the investigated probiotic formulation did not alter the intestinal bacterial ecosystem of healthy adults and suggest its potential ability to promote colonization resistance in the gut through a transient increase in fecal bifidobacteria, lactic acid, and the acetate/butyrate ratio. View Full-Text
Keywords: DESeq2; ALDEx2; short-chain fatty acids; succinate; acetate; butyrate; lactate; fecal microbiota; intervention trial DESeq2; ALDEx2; short-chain fatty acids; succinate; acetate; butyrate; lactate; fecal microbiota; intervention trial
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MDPI and ACS Style

Gargari, G.; Taverniti, V.; Koirala, R.; Gardana, C.; Guglielmetti, S. Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults. Microorganisms 2020, 8, 492. https://doi.org/10.3390/microorganisms8040492

AMA Style

Gargari G, Taverniti V, Koirala R, Gardana C, Guglielmetti S. Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults. Microorganisms. 2020; 8(4):492. https://doi.org/10.3390/microorganisms8040492

Chicago/Turabian Style

Gargari, Giorgio, Valentina Taverniti, Ranjan Koirala, Claudio Gardana, and Simone Guglielmetti. 2020. "Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults" Microorganisms 8, no. 4: 492. https://doi.org/10.3390/microorganisms8040492

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