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Open AccessArticle

Polyphasic Characterisation of Cedecea colo sp. nov., a New Enteric Bacterium Isolated from the Koala Hindgut

1
School of Science, RMIT University, Bundoora, Victoria 3083, Australia
2
Australian Microscopy & Microanalysis Research Facility, RMIT University, Melbourne, Victoria 3000, Australia
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(2), 309; https://doi.org/10.3390/microorganisms8020309
Received: 6 February 2020 / Revised: 18 February 2020 / Accepted: 22 February 2020 / Published: 24 February 2020
The Cedecea genus is comprised of six rarely isolated species within the Enterobacteriaceae family. Representatives are Gram-negative motile bacilli, and are typically oxidase-negative, lipase-positive and resistant to colistin and cephalothin. In this study, a putative novel Cedecea species (designated strain ZA_0188T), isolated from the koala hindgut, was characterised using a polyphasic taxonomic approach. Maximum average nucleotide identity (ANI) and 16S ribosomal RNA (rRNA) similarity scores well below thresholds of species demarcation were reported, at 81.1% and 97.9%, respectively. Multilocus phylogenetic analysis indicated strain ZA_0188T was most similar to but divergent from recognised Cedecea species. The isolate’s genomic G+C content was determined as 53.0 mol%, >1% lower than previously reported in Cedecea. Phenotypically, strain ZA_0188T was distinct from recognised Cedecea species such as colistin- and cephalothin-sensitive, lipase-, sorbitol-, sucrose-, and Voges-Proskauer-negative, and melibiose-, arabinose-, arginine-, and rhamnose-positive. In preliminary experiments, strain ZA_0188T exhibited cellulase activity and high-level tolerance to eucalyptus oil compared to other enteric species surveyed. Collectively, these findings suggest that strain ZA_0188T represents a novel enteric species, for which the name Cedecea colo is proposed. View Full-Text
Keywords: koala microbiome; polyphasic taxonomy; extremophiles; novel species koala microbiome; polyphasic taxonomy; extremophiles; novel species
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MDPI and ACS Style

Boath, J.M.; Dakhal, S.; Van, T.T.H.; Moore, R.J.; Dekiwadia, C.; Macreadie, I.G. Polyphasic Characterisation of Cedecea colo sp. nov., a New Enteric Bacterium Isolated from the Koala Hindgut. Microorganisms 2020, 8, 309. https://doi.org/10.3390/microorganisms8020309

AMA Style

Boath JM, Dakhal S, Van TTH, Moore RJ, Dekiwadia C, Macreadie IG. Polyphasic Characterisation of Cedecea colo sp. nov., a New Enteric Bacterium Isolated from the Koala Hindgut. Microorganisms. 2020; 8(2):309. https://doi.org/10.3390/microorganisms8020309

Chicago/Turabian Style

Boath, Jarryd M.; Dakhal, Sudip; Van, Thi T.H.; Moore, Robert J.; Dekiwadia, Chaitali; Macreadie, Ian G. 2020. "Polyphasic Characterisation of Cedecea colo sp. nov., a New Enteric Bacterium Isolated from the Koala Hindgut" Microorganisms 8, no. 2: 309. https://doi.org/10.3390/microorganisms8020309

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