Next Article in Journal
Long-Term Mesophilic Anaerobic Co-Digestion of Swine Manure with Corn Stover and Microbial Community Analysis
Previous Article in Journal
Model-Driven Controlled Alteration of Nanopillar Cap Architecture Reveals its Effects on Bactericidal Activity
Article

Uptake of Inorganic and Organic Nitrogen Sources by Dinophysis acuminata and D. acuta

1
Spanish Institute of Oceanography (IEO), Oceanographic Center of Vigo, Subida a Radio Faro 50, Cabo Estay, Canido, 36390 Vigo, Spain
2
Ifremer, DYNECO PELAGOS, 29280 Plouzané, France
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(2), 187; https://doi.org/10.3390/microorganisms8020187
Received: 17 December 2019 / Revised: 20 January 2020 / Accepted: 25 January 2020 / Published: 29 January 2020
(This article belongs to the Section Environmental Microbiology)
Dinoflagellate species of Dinophysis are obligate mixotrophs that require light, nutrients, and prey for sustained growth. Information about their nitrogenous nutrient preferences and their uptake kinetics are scarce. This study aimed to determine the preferred nitrogen sources in cultures of D. acuminata and D. acuta strains from the Galician Rías Baixas (NW Spain) and to compare their uptake kinetics. Well-fed versus starved cultures of D. acuminata and D. acuta were supplied with N15 labeled inorganic (nitrate, ammonium) and organic (urea) nutrients. Both species showed a preference for ammonium and urea whereas uptake of nitrate was negligible. Uptake rates by well-fed cells of D. acuminata and D. acuta were 200% and 50% higher, respectively, than by starved cells. Uptake of urea by D. acuminata was significantly higher than that of ammonium in both nutritional conditions. In contrast, similar uptake rates of both compounds were observed in D. acuta. The apparent inability of Dinophysis to take up nitrate suggests the existence of incomplete nitrate-reducing and assimilatory pathways, in line with the paucity of nitrate transporter homologs in the D. acuminata reference transcriptome. Results derived from this study will contribute to understand Harmful Algal Blooms succession and differences in the spatio-temporal distribution of the two Dinophysis species when they co-occur in stratified scenarios. View Full-Text
Keywords: Dinophysis; nitrate; ammonium; urea; uptake rates; N15 incubations; antibiotic treatment Dinophysis; nitrate; ammonium; urea; uptake rates; N15 incubations; antibiotic treatment
Show Figures

Figure 1

MDPI and ACS Style

García-Portela, M.; Reguera, B.; Gago, J.; Le Gac, M.; Rodríguez, F. Uptake of Inorganic and Organic Nitrogen Sources by Dinophysis acuminata and D. acuta. Microorganisms 2020, 8, 187. https://doi.org/10.3390/microorganisms8020187

AMA Style

García-Portela M, Reguera B, Gago J, Le Gac M, Rodríguez F. Uptake of Inorganic and Organic Nitrogen Sources by Dinophysis acuminata and D. acuta. Microorganisms. 2020; 8(2):187. https://doi.org/10.3390/microorganisms8020187

Chicago/Turabian Style

García-Portela, María, Beatriz Reguera, Jesús Gago, Mickael Le Gac, and Francisco Rodríguez. 2020. "Uptake of Inorganic and Organic Nitrogen Sources by Dinophysis acuminata and D. acuta" Microorganisms 8, no. 2: 187. https://doi.org/10.3390/microorganisms8020187

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop