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Microorganisms
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10.3390/microorganisms8122001
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Article

Establishment of a Challenge Model for Sheeppox Virus Infection

by
Janika Wolff
1,
Sahar Abd El Rahman
2,
Jacqueline King
1,
Mohamed El-Beskawy
3,
Anne Pohlmann
1,
Martin Beer
1 and
Bernd Hoffmann
1,*
1
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, Insel Riems, D-17493 Greifswald, Germany
2
Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
3
Department of Animal Medicine, Faculty of Veterinary Medicine, Matrouh University, Matrouh 51744, Egypt
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(12), 2001; https://doi.org/10.3390/microorganisms8122001
Submission received: 2 November 2020 / Revised: 8 December 2020 / Accepted: 12 December 2020 / Published: 15 December 2020
(This article belongs to the Special Issue Capripoxviruses: A Continuing Worldwide Threat to Sheep, Goats and Cattle)
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Abstract

Sheeppox virus (SPPV) together with goatpox virus and lumpy skin disease virus form the genus Capripoxvirus of the Poxviridae family. Due to their great economic importance and major impact on livelihood of small-scale farmers, OIE guidelines classify capripox viruses as notifiable diseases. In the present study, we examined pathogenesis of an Indian SPPV isolate and an Egyptian SPPV isolate in sheep. Three different infection routes were tested: (i) intravenous infection, (ii) intranasal infection and (iii) contact transmission between infected and naïve sheep. Clinical course, viremia and viral shedding as well as seroconversion were analyzed in order to establish a challenge model for SPPV infections that can be used in future vaccine studies. Next to in vivo characterization, both SPPV strains underwent next- and third-generation sequencing to obtain high quality full-length genomes for genetic characterization and comparison to already published SPPV sequences.
Keywords: capripox; sheeppox; SPPV; field strain; pathogenesis; challenge model; nanopore sequencing; next-generation sequencing; MinION capripox; sheeppox; SPPV; field strain; pathogenesis; challenge model; nanopore sequencing; next-generation sequencing; MinION

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MDPI and ACS Style

Wolff, J.; Abd El Rahman, S.; King, J.; El-Beskawy, M.; Pohlmann, A.; Beer, M.; Hoffmann, B. Establishment of a Challenge Model for Sheeppox Virus Infection. Microorganisms 2020, 8, 2001. https://doi.org/10.3390/microorganisms8122001

AMA Style

Wolff J, Abd El Rahman S, King J, El-Beskawy M, Pohlmann A, Beer M, Hoffmann B. Establishment of a Challenge Model for Sheeppox Virus Infection. Microorganisms. 2020; 8(12):2001. https://doi.org/10.3390/microorganisms8122001

Chicago/Turabian Style

Wolff, Janika, Sahar Abd El Rahman, Jacqueline King, Mohamed El-Beskawy, Anne Pohlmann, Martin Beer, and Bernd Hoffmann. 2020. "Establishment of a Challenge Model for Sheeppox Virus Infection" Microorganisms 8, no. 12: 2001. https://doi.org/10.3390/microorganisms8122001

APA Style

Wolff, J., Abd El Rahman, S., King, J., El-Beskawy, M., Pohlmann, A., Beer, M., & Hoffmann, B. (2020). Establishment of a Challenge Model for Sheeppox Virus Infection. Microorganisms, 8(12), 2001. https://doi.org/10.3390/microorganisms8122001

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