Next Article in Journal
Virulence of Francisella tularensis Subspecies holarctica Biovar japonica and Phenotypic Change during Serial Passages on Artificial Media
Next Article in Special Issue
Anaerobic Carbon Monoxide Uptake by Microbial Communities in Volcanic Deposits at Different Stages of Successional Development on O-yama Volcano, Miyake-jima, Japan
Previous Article in Journal
A Role for the Microbiota in the Immune Phenotype Alteration Associated with the Induction of Disease Tolerance and Persistent Asymptomatic Infection of Salmonella in the Chicken
Previous Article in Special Issue
Sphingopyxis sp. Strain OPL5, an Isoprene-Degrading Bacterium from the Sphingomonadaceae Family Isolated from Oil Palm Leaves
Open AccessArticle

Reconstructing Genomes of Carbon Monoxide Oxidisers in Volcanic Deposits Including Members of the Class Ktedonobacteria

1
Johann Heinrich von Thünen Institut, Institut für Biodiversität, 50, 38116 Braunschweig, Germany
2
School of Environmental Sciences, Norwich Research Park, University of East Anglia, Norwich NR4 7TJ, UK
3
Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany
4
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain
5
BIOREN-UFRO, Universidad de La Frontera, Temuco 4811230, Chile
6
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(12), 1880; https://doi.org/10.3390/microorganisms8121880
Received: 28 October 2020 / Revised: 13 November 2020 / Accepted: 24 November 2020 / Published: 27 November 2020
(This article belongs to the Special Issue Microbial Cycling of Atmospheric Trace Gases)
Microorganisms can potentially colonise volcanic rocks using the chemical energy in reduced gases such as methane, hydrogen (H2) and carbon monoxide (CO). In this study, we analysed soil metagenomes from Chilean volcanic soils, representing three different successional stages with ages of 380, 269 and 63 years, respectively. A total of 19 metagenome-assembled genomes (MAGs) were retrieved from all stages with a higher number observed in the youngest soil (1640: 2 MAGs, 1751: 1 MAG, 1957: 16 MAGs). Genomic similarity indices showed that several MAGs had amino-acid identity (AAI) values >50% to the phyla Actinobacteria, Acidobacteria, Gemmatimonadetes, Proteobacteria and Chloroflexi. Three MAGs from the youngest site (1957) belonged to the class Ktedonobacteria (Chloroflexi). Complete cellular functions of all the MAGs were characterised, including carbon fixation, terpenoid backbone biosynthesis, formate oxidation and CO oxidation. All 19 environmental genomes contained at least one gene encoding a putative carbon monoxide dehydrogenase (CODH). Three MAGs had form I coxL operon (encoding the large subunit CO-dehydrogenase). One of these MAGs (MAG-1957-2.1, Ktedonobacterales) was highly abundant in the youngest soil. MAG-1957-2.1 also contained genes encoding a [NiFe]-hydrogenase and hyp genes encoding accessory enzymes and proteins. Little is known about the Ktedonobacterales through cultivated isolates, but some species can utilise H2 and CO for growth. Our results strongly suggest that the remote volcanic sites in Chile represent a natural habitat for Ktedonobacteria and they may use reduced gases for growth. View Full-Text
Keywords: Ktedonobacteria; carbon monoxide dehydrogenase; metagenome-assembled-genome; volcanic soils; shotgun sequencing Ktedonobacteria; carbon monoxide dehydrogenase; metagenome-assembled-genome; volcanic soils; shotgun sequencing
Show Figures

Figure 1

MDPI and ACS Style

Hernández, M.; Vera-Gargallo, B.; Calabi-Floody, M.; King, G.M.; Conrad, R.; Tebbe, C.C. Reconstructing Genomes of Carbon Monoxide Oxidisers in Volcanic Deposits Including Members of the Class Ktedonobacteria. Microorganisms 2020, 8, 1880. https://doi.org/10.3390/microorganisms8121880

AMA Style

Hernández M, Vera-Gargallo B, Calabi-Floody M, King GM, Conrad R, Tebbe CC. Reconstructing Genomes of Carbon Monoxide Oxidisers in Volcanic Deposits Including Members of the Class Ktedonobacteria. Microorganisms. 2020; 8(12):1880. https://doi.org/10.3390/microorganisms8121880

Chicago/Turabian Style

Hernández, Marcela; Vera-Gargallo, Blanca; Calabi-Floody, Marcela; King, Gary M.; Conrad, Ralf; Tebbe, Christoph C. 2020. "Reconstructing Genomes of Carbon Monoxide Oxidisers in Volcanic Deposits Including Members of the Class Ktedonobacteria" Microorganisms 8, no. 12: 1880. https://doi.org/10.3390/microorganisms8121880

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop