Next Article in Journal
Reconstructing Genomes of Carbon Monoxide Oxidisers in Volcanic Deposits Including Members of the Class Ktedonobacteria
Next Article in Special Issue
Dual Inhibition of Salmonella enterica and Clostridium perfringens by New Probiotic Candidates Isolated from Chicken Intestinal Mucosa
Previous Article in Journal
Resolved Influenza A Virus Infection Has Extended Effects on Lung Homeostasis and Attenuates Allergic Airway Inflammation in a Mouse Model
Previous Article in Special Issue
Occurrence of tet(O/M/O) Mosaic Gene in Tetracycline-Resistant Campylobacter
Open AccessArticle

A Role for the Microbiota in the Immune Phenotype Alteration Associated with the Induction of Disease Tolerance and Persistent Asymptomatic Infection of Salmonella in the Chicken

1
Department of Poultry Science, Texas A&M University, College Station, TX 77843, USA
2
Department of Small Animal Clinical Sciences, Texas A&M University, College Station, TX 77843, USA
3
Food and Feed Safety Research Unit, United States Department of Agriculture/Agriculture Research Service, College Station, TX 77845, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(12), 1879; https://doi.org/10.3390/microorganisms8121879
Received: 4 October 2020 / Revised: 25 November 2020 / Accepted: 26 November 2020 / Published: 27 November 2020
Previous studies have shown a tissue immune phenotype-altering event occurring on days 2 and 4 in the ceca post-Salmonella challenge. To evaluate the involvement of the cecal microbiota in the phenotype reprogramming, we hypothesized that the addition of subtherapeutic bacitracin (BMD) will affect the cecal microbiota. Therefore, the objective of this study was to determine if the antibiotic-mediated changes in the microbiota composition influenced the immune phenotype induced by Salmonella enteritidis infection of the chicken cecum. A total of 112 fertile eggs were obtained for each experiment, repeated for a total of three separate times. The ceca and cecal contents were collected on days 2 and 4 post-infection for mRNA expression TaqMan assay and 16S rRNA gene microbiota sequencing. The results demonstrate the effects of bacitracin on cecal composition and its interaction with Salmonella enteritidis in young chicks. There is a preliminary indication of phenotype change in the Salmonella-challenged group provided subtherapeutic BMD due to the shifting cecal microbiota and cecal immune response, indicating the addition of bacitracin during infection altered the cecal phenotype. These data demonstrate the potential involvement of the microbiota in reprogramming immune phenotype (disease resistance to disease tolerance) induced by Salmonella in the chicken cecum. View Full-Text
Keywords: Salmonella enteritidis; gut microbiota; antibiotics; immune phenotype Salmonella enteritidis; gut microbiota; antibiotics; immune phenotype
Show Figures

Figure 1

MDPI and ACS Style

Lee, A.; Bortoluzzi, C.; Pilla, R.; Kogut, M.H. A Role for the Microbiota in the Immune Phenotype Alteration Associated with the Induction of Disease Tolerance and Persistent Asymptomatic Infection of Salmonella in the Chicken. Microorganisms 2020, 8, 1879. https://doi.org/10.3390/microorganisms8121879

AMA Style

Lee A, Bortoluzzi C, Pilla R, Kogut MH. A Role for the Microbiota in the Immune Phenotype Alteration Associated with the Induction of Disease Tolerance and Persistent Asymptomatic Infection of Salmonella in the Chicken. Microorganisms. 2020; 8(12):1879. https://doi.org/10.3390/microorganisms8121879

Chicago/Turabian Style

Lee, Annah; Bortoluzzi, Cristiano; Pilla, Rachel; Kogut, Michael H. 2020. "A Role for the Microbiota in the Immune Phenotype Alteration Associated with the Induction of Disease Tolerance and Persistent Asymptomatic Infection of Salmonella in the Chicken" Microorganisms 8, no. 12: 1879. https://doi.org/10.3390/microorganisms8121879

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop