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Open AccessArticle

Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus

1
Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001–0020, Japan
2
Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
3
Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
4
Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo 001–0020, Japan
*
Author to whom correspondence should be addressed.
Current address: Men’s College, Higher Colleges of Technology, University City Sharjah, Sharjah 7946, UAE.
Current address: Department of Pathology, The University of Texas Medical Branch, Galveston, TX 77555, USA.
§
Current address: Tsukuba Branch, WDB Co., Ltd., 1–6–1 Takezono, Ibaraki, Tsukuba 305–0032, Japan.
Current address: Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700–8558, Japan.
Microorganisms 2020, 8(10), 1530; https://doi.org/10.3390/microorganisms8101530
Received: 30 July 2020 / Revised: 30 September 2020 / Accepted: 1 October 2020 / Published: 5 October 2020
(This article belongs to the Special Issue Hemorrhagic Fever Viruses: Pathogenesis and Countermeasures)
Lloviu virus (LLOV), a bat-derived filovirus that is phylogenetically distinct from human pathogenic filoviruses such as Ebola virus (EBOV) and Marburg virus (MARV), was discovered in Europe. However, since infectious LLOV has never been isolated, the biological properties of this virus remain poorly understood. We found that vesicular stomatitis virus (VSV) pseudotyped with the glycoprotein (GP) of LLOV (VSV–LLOV) showed higher infectivity in one bat (Miniopterus sp.)-derived cell line than in the other bat-derived cell lines tested, which was distinct from the tropism of VSV pseudotyped with EBOV (VSV–EBOV) and MARV GPs. We then focused on the interaction between GP and Niemann–Pick C1 (NPC1) protein, one of the cellular receptors of filoviruses. We introduced the Miniopterus bat and human NPC1 genes into NPC1-knockout Vero E6 cells and their susceptibilities to the viruses were compared. The cell line expressing the bat NPC1 showed higher susceptibility to VSV–LLOV than that expressing human NPC1, whereas the opposite preference was seen for VSV–EBOV. Using a site-directed mutagenesis approach, amino acid residues involved in the differential tropism were identified in the NPC1 and GP molecules. Our results suggest that the interaction between GP and NPC1 is an important factor in the tropism of LLOV to a particular bat species. View Full-Text
Keywords: filovirus; lloviu virus; Miniopterus sp.; bat; insectivorous bat; host range; Niemann–Pick C1; glycoprotein filovirus; lloviu virus; Miniopterus sp.; bat; insectivorous bat; host range; Niemann–Pick C1; glycoprotein
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MDPI and ACS Style

Takadate, Y.; Manzoor, R.; Saito, T.; Kida, Y.; Maruyama, J.; Kondoh, T.; Miyamoto, H.; Ogawa, H.; Kajihara, M.; Igarashi, M.; Takada, A. Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus. Microorganisms 2020, 8, 1530. https://doi.org/10.3390/microorganisms8101530

AMA Style

Takadate Y, Manzoor R, Saito T, Kida Y, Maruyama J, Kondoh T, Miyamoto H, Ogawa H, Kajihara M, Igarashi M, Takada A. Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus. Microorganisms. 2020; 8(10):1530. https://doi.org/10.3390/microorganisms8101530

Chicago/Turabian Style

Takadate, Yoshihiro; Manzoor, Rashid; Saito, Takeshi; Kida, Yurie; Maruyama, Junki; Kondoh, Tatsunari; Miyamoto, Hiroko; Ogawa, Hirohito; Kajihara, Masahiro; Igarashi, Manabu; Takada, Ayato. 2020. "Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus" Microorganisms 8, no. 10: 1530. https://doi.org/10.3390/microorganisms8101530

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