Hafnia alvei HA4597 Strain Reduces Food Intake and Body Weight Gain and Improves Body Composition, Glucose, and Lipid Metabolism in a Mouse Model of Hyperphagic Obesity
by
Nicolas Lucas 1,†, Romain Legrand 1,†
, Camille Deroissart 1,2,3, Manon Dominique 1,2,3, Saïda Azhar 1, Marie-Anne Le Solliec 1,2,3, Fatima Léon 3,4, Jean-Claude do Rego 3,4, Pierre Déchelotte 1,2,3,5, Sergueï O. Fetissov 1,3,6,* and 
Grégory Lambert 1,*
Nicolas Lucas 1,†, Romain Legrand 1,†, Camille Deroissart 1,2,3, Manon Dominique 1,2,3, Saïda Azhar 1, Marie-Anne Le Solliec 1,2,3, Fatima Léon 3,4, Jean-Claude do Rego 3,4, Pierre Déchelotte 1,2,3,5, Sergueï O. Fetissov 1,3,6,* and Grégory Lambert 1,*
1
TargEDys SA, University of Rouen Normandy, 22, Boulevard Gambetta, 76183 Rouen, CEDEX 1, France
2
Inserm UMR1073, Nutrition, Gut and Brain Laboratory, 76183 Rouen, France
3
Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen Normandy, 76183 Rouen, France
4
Animal Behavioral Platform, SCAC, University of Rouen Normandy, 76183 Rouen, France
5
Rouen University Hospital, CHU Charles Nicolle, 76183 Rouen, France
6
Inserm UMR1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication, University of Rouen Normandy, 76130 Mont-Saint-Aignan, France
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2020, 8(1), 35; https://doi.org/10.3390/microorganisms8010035
Received: 26 November 2019 / Revised: 13 December 2019 / Accepted: 19 December 2019 / Published: 23 December 2019
(This article belongs to the Special Issue Host-Gut Microbiota Interactions)
Use of new generation probiotics may become an integral part of the prevention and treatment strategies of obesity. The aim of the present study was to test the efficacy of a potential probiotic strain of lactic bacteria Hafnia alvei (H. alvei) HA4597™, in a mouse model of obesity characterized by both hyperphagia and diet-induced adiposity. For this purpose, 10-week-old high-fat-diet (HFD)-fed hyperphagic ob/ob male mice received a daily treatment with 1.4 × 1010 CFU of H. alvei for 38 days. Effects of H. alvei were compared to those of a lipase inhibitor orlistat (80 mg/kg daily) and a vehicle (NaCl 0.9%) in HFD-fed ob/ob mice. A control untreated group of ob/ob mice received the standard diet throughout the experiment. The vehicle-treated HFD group displayed increased food intake, worsening of adiposity, and glycemia. Treatment with H. alvei was accompanied by decreased body weight and fat-mass gain along with reduced food intake to the level of the standard-diet-fed mice. At the end of the experiment, the group treated with H. alvei showed a decrease of glycemia, plasma total cholesterol, and alanine aminotransferase. The orlistat-treated mice showed a lower rate of body weight gain but were hyperphagic and hyperglycemic. These results demonstrate the beneficial anti-obesity and metabolic effects of H. alvei HA4597™ in mice with obesity resulting from hyperphagia and diet-induced adiposity.
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Keywords:
obesity; food intake; microbiota; probiotics; Hafnia alvei; animal models
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MDPI and ACS Style
Lucas, N.; Legrand, R.; Deroissart, C.; Dominique, M.; Azhar, S.; Le Solliec, M.-A.; Léon, F.; do Rego, J.-C.; Déchelotte, P.; Fetissov, S.O.; Lambert, G. Hafnia alvei HA4597 Strain Reduces Food Intake and Body Weight Gain and Improves Body Composition, Glucose, and Lipid Metabolism in a Mouse Model of Hyperphagic Obesity. Microorganisms 2020, 8, 35.
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