Next Article in Journal
Mode of Action of Dietary Dexamethasone May Not Be Dependent Upon Microbial Mechanisms in Broilers
Next Article in Special Issue
Probiotic Bifunctionality of Bacillus subtilis—Rescuing Lactic Acid Bacteria from Desiccation and Antagonizing Pathogenic Staphylococcus aureus
Previous Article in Journal
Characterization of mcr-1-Harboring Plasmids from Pan Drug-Resistant Escherichia coli Strains Isolated from Retail Raw Chicken in South Korea
Previous Article in Special Issue
Antibacterial Activity of Three Extra Virgin Olive Oils of the Campania Region, Southern Italy, Related to Their Polyphenol Content and Composition
Article

Anti-Biofilm Effect of Selected Essential Oils and Main Components on Mono- and Polymicrobic Bacterial Cultures

1
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Közép fasor 52, Hungary
2
Institute of Food Engineering, Faculty of Engineering, University of Szeged, H-6724 Szeged, Mars tér 7, Hungary
3
Department of Pharmacognosy, University of Pécs, H-7624 Pécs, Rókus utca 2, Hungary
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(9), 345; https://doi.org/10.3390/microorganisms7090345
Received: 26 July 2019 / Revised: 23 August 2019 / Accepted: 10 September 2019 / Published: 12 September 2019
(This article belongs to the Special Issue Natural Alternatives against Bacterial Foodborne Pathogens)
Biofilms are surface-associated microbial communities resistant to sanitizers and antimicrobials. Various interactions that can contribute to increased resistance occur between the populations in biofilms. These relationships are the focus of a range of studies dealing with biofilm-associated infections and food spoilage. The present study investigated the effects of cinnamon (Cinnamomum zeylanicum), marjoram (Origanum majorana), and thyme (Thymus vulgaris) essential oils (EOs) and their main components, i.e., trans-cinnamaldehyde, terpinen-4-ol, and thymol, respectively, on single- and dual-species biofilms of Escherichia coli, Listeria monocytogenes, Pseudomonas putida, and Staphylococcus aureus. In dual-species biofilms, L. monocytogenes was paired with each of the other three bacteria. Minimum inhibitory concentration (MIC) values for the individual bacteria ranged between 0.25 and 20 mg/mL, and trans-cinnamaldehyde and cinnamon showed the highest growth inhibitory effect. Single-species biofilms of L. monocytogenes, P. putida, and S. aureus were inhibited by the tested EOs and their components at sub-lethal concentrations. Scanning electron microscopy images showed that the three-dimensional structure of mature biofilms embedded in the exopolysaccharide matrix disappeared or was limited to micro-colonies with a simplified structure. In most dual-species biofilms, to eliminate living cells from the matrix, concentrations exceeding the MIC determined for individual bacteria were required. View Full-Text
Keywords: antibacterial activity; biofilm; polymicrobial biofilm; essential oil; food spoilage antibacterial activity; biofilm; polymicrobial biofilm; essential oil; food spoilage
Show Figures

Figure 1

MDPI and ACS Style

Kerekes, E.B.; Vidács, A.; Takó, M.; Petkovits, T.; Vágvölgyi, C.; Horváth, G.; Balázs, V.L.; Krisch, J. Anti-Biofilm Effect of Selected Essential Oils and Main Components on Mono- and Polymicrobic Bacterial Cultures. Microorganisms 2019, 7, 345. https://doi.org/10.3390/microorganisms7090345

AMA Style

Kerekes EB, Vidács A, Takó M, Petkovits T, Vágvölgyi C, Horváth G, Balázs VL, Krisch J. Anti-Biofilm Effect of Selected Essential Oils and Main Components on Mono- and Polymicrobic Bacterial Cultures. Microorganisms. 2019; 7(9):345. https://doi.org/10.3390/microorganisms7090345

Chicago/Turabian Style

Kerekes, Erika B., Anita Vidács, Miklós Takó, Tamás Petkovits, Csaba Vágvölgyi, Györgyi Horváth, Viktória L. Balázs, and Judit Krisch. 2019. "Anti-Biofilm Effect of Selected Essential Oils and Main Components on Mono- and Polymicrobic Bacterial Cultures" Microorganisms 7, no. 9: 345. https://doi.org/10.3390/microorganisms7090345

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop