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Open AccessArticle

Dual RNA-Seq Analysis of the Pine-Fusarium circinatum Interaction in Resistant (Pinus tecunumanii) and Susceptible (Pinus patula) Hosts

1
Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Centre for Bioinformatics and Computational Biology, University of Pretoria, Private Bag X20, Pretoria 0028, South Africa
2
Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT 06269, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(9), 315; https://doi.org/10.3390/microorganisms7090315
Received: 3 July 2019 / Revised: 15 August 2019 / Accepted: 20 August 2019 / Published: 4 September 2019
(This article belongs to the Special Issue Ecology and Genomics of Forest Fungi and Their Interactions)
Fusarium circinatum poses a serious threat to many pine species in both commercial and natural pine forests. Knowledge regarding the molecular basis of pine-F. circinatum host-pathogen interactions could assist efforts to produce more resistant planting stock. This study aimed to identify molecular responses underlying resistance against F. circinatum. A dual RNA-seq approach was used to investigate host and pathogen expression in F. circinatum challenged Pinus tecunumanii (resistant) and Pinus patula (susceptible), at three- and seven-days post inoculation. RNA-seq reads were mapped to combined host-pathogen references for both pine species to identify differentially expressed genes (DEGs). F. circinatum genes expressed during infection showed decreased ergosterol biosynthesis in P. tecunumanii relative to P. patula. For P. tecunumanii, enriched gene ontologies and DEGs indicated roles for auxin-, ethylene-, jasmonate- and salicylate-mediated phytohormone signalling. Correspondingly, key phytohormone signaling components were down-regulated in P. patula. Key F. circinatum ergosterol biosynthesis genes were expressed at lower levels during infection of the resistant relative to the susceptible host. This study further suggests that coordination of phytohormone signaling is required for F. circinatum resistance in P. tecunumanii, while a comparatively delayed response and impaired phytohormone signaling contributes to susceptibility in P. patula. View Full-Text
Keywords: Dual RNA-seq; ergosterol biosynthesis; Fusarium circinatum; host-pathogen interaction; Pinus patula; Pinus tecunumanii Dual RNA-seq; ergosterol biosynthesis; Fusarium circinatum; host-pathogen interaction; Pinus patula; Pinus tecunumanii
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MDPI and ACS Style

Visser, E.A.; Wegrzyn, J.L.; Steenkamp, E.T.; Myburg, A.A.; Naidoo, S. Dual RNA-Seq Analysis of the Pine-Fusarium circinatum Interaction in Resistant (Pinus tecunumanii) and Susceptible (Pinus patula) Hosts. Microorganisms 2019, 7, 315. https://doi.org/10.3390/microorganisms7090315

AMA Style

Visser EA, Wegrzyn JL, Steenkamp ET, Myburg AA, Naidoo S. Dual RNA-Seq Analysis of the Pine-Fusarium circinatum Interaction in Resistant (Pinus tecunumanii) and Susceptible (Pinus patula) Hosts. Microorganisms. 2019; 7(9):315. https://doi.org/10.3390/microorganisms7090315

Chicago/Turabian Style

Visser, Erik A.; Wegrzyn, Jill L.; Steenkamp, Emma T.; Myburg, Alexander A.; Naidoo, Sanushka. 2019. "Dual RNA-Seq Analysis of the Pine-Fusarium circinatum Interaction in Resistant (Pinus tecunumanii) and Susceptible (Pinus patula) Hosts" Microorganisms 7, no. 9: 315. https://doi.org/10.3390/microorganisms7090315

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