Next Article in Journal
Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains
Next Article in Special Issue
Pathobiology of Aspergillus Fumigatus Endophthalmitis in Immunocompetent and Immunocompromised Mice
Previous Article in Journal
Arbuscular Mycorrhiza Enhances Biomass Production and Salt Tolerance of Sweet Sorghum
Previous Article in Special Issue
Pseudomonas aeruginosa Keratitis: Protease IV and PASP as Corneal Virulence Mediators
Open AccessArticle

A Transcriptional Activator of Ascorbic Acid Transport in Streptococcus pneumoniae Is Required for Optimal Growth in Endophthalmitis in a Strain-Dependent Manner

1
Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, MS 39216, USA
2
Current affiliation: Virginia Tech, Blacksburg, VA 24061, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(9), 290; https://doi.org/10.3390/microorganisms7090290
Received: 29 July 2019 / Revised: 18 August 2019 / Accepted: 21 August 2019 / Published: 24 August 2019
(This article belongs to the Special Issue Insights Into The Molecular Pathogenesis of Ocular Infections)
Streptococcus pneumoniae is among the top causes of bacterial endophthalmitis, an infectious disease of the intraocular fluids. The mechanisms by which S. pneumoniae grows and thrives in the intraocular cavity are not well understood. We used a bacterial genome-wide assessment tool (transposon insertion site sequencing) to determine genes essential for S. pneumoniae growth in vitreous humor. The results indicated that an ascorbic acid (AA) transport system subunit was important for growth. We created an isogenic gene deletion mutant of the AA transcriptional activator, ulaR2, in 2 strains of S. pneumoniae. Growth curve analysis indicated that ulaR2 deletion caused attenuated growth in vitro for both strains. However, in vivo vitreous humor infection in rabbits with either strain determined that ulaR2 was necessary for growth in one strain but not the other. These results demonstrate that ulaR2 may be important for fitness during S. pneumoniae endophthalmitis depending on the background of the strain. View Full-Text
Keywords: ascorbic acid; endophthalmitis; Streptococcus pneumoniae ascorbic acid; endophthalmitis; Streptococcus pneumoniae
Show Figures

Figure 1

MDPI and ACS Style

Benton, A.H.; Jackson, M.D.; Wong, S.M.; Dees, J.L.; Akerley, B.J.; Marquart, M.E. A Transcriptional Activator of Ascorbic Acid Transport in Streptococcus pneumoniae Is Required for Optimal Growth in Endophthalmitis in a Strain-Dependent Manner. Microorganisms 2019, 7, 290.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop