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Open AccessArticle

Disinfectant Susceptibility of Biofilm Formed by Listeria monocytogenes under Selected Environmental Conditions

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Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, Poland
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Department of Food Hygiene and Consumer Health, Wrocław University of Environmental and Life Sciences, 31 C.K. Norwida St., 50-375 Wrocław, Poland
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Department of Theoretical Foundations of Biomedical Sciences and Medical Computer Science, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, Poland
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Faculty of Telecommunication, Information Technology and Electrical Engineering, Institute of Telecommunications and Computer Science, UTP University of Science and Technology, Al. prof. S. Kaliskiego 7, 85-796 Bydgoszcz, Poland
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Author to whom correspondence should be addressed.
Microorganisms 2019, 7(9), 280; https://doi.org/10.3390/microorganisms7090280
Received: 13 July 2019 / Revised: 16 August 2019 / Accepted: 19 August 2019 / Published: 21 August 2019
(This article belongs to the Special Issue Bacterial Biofilms and Its Eradication in Food Industry)
Listeria monocytogenes is a one of the most important food-borne pathogens. Its ability to form biofilm contributes to increased resistance to disinfectants and inefficient disinfection, posing a serious threat for the food industry, and in the end the consumer. The aim of this study was the comparison of the biofilm formation ability of L. monocytogenes strains on stainless steel, under different environmental conditions (temperature, pH, NaCl concentration, nutrients availability), and the assessment of biofilm susceptibility to disinfectants. The bactericidal activity of four disinfectants in two concentrations (100% and 50% of working solution) against biofilm was conducted on four clinical strains, four strains isolated from food and one reference strain ATCC 19111. It was found that biofilm susceptibility to disinfectants was influenced by environmental conditions. Biofilm susceptibility correlated with the decrease of temperature, pH, nutrients availability and salinity of the environment. The least sensitive to disinfectants was biofilm produced at pH = 4 (the bacterial number ranged from 0.25 log CFU × cm−2 to 1.72 log CFU × cm−2) whereas the most sensitive was biofilm produced at pH = 9 (5.16 log CFU × cm−2 to 7.84 log CFU × cm−2). Quatosept was the most effective disinfectant, regardless of the conditions. In conclusion, biofilm susceptibility to disinfectants is strain-dependent and is affected by environmental conditions. View Full-Text
Keywords: L. monocytogenes; biofilm formation; biofilm resistance; disinfectants; stainless steel; environmental conditions L. monocytogenes; biofilm formation; biofilm resistance; disinfectants; stainless steel; environmental conditions
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MDPI and ACS Style

Skowron, K.; Wałecka-Zacharska, E.; Grudlewska, K.; Gajewski, P.; Wiktorczyk, N.; Wietlicka-Piszcz, M.; Dudek, A.; Skowron, K.J.; Gospodarek-Komkowska, E. Disinfectant Susceptibility of Biofilm Formed by Listeria monocytogenes under Selected Environmental Conditions. Microorganisms 2019, 7, 280.

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