Improved Positive Predictive Performance of Listeria Indicator Broth: A Sensitive Environmental Screening Test to Identify Presumptively Positive Swab Samples

Olstein, Alan D.; Feirtag, Joellen M.

doi:10.3390/microorganisms7050151

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Improved Positive Predictive Performance of Listeria Indicator Broth: A Sensitive Environmental Screening Test to Identify Presumptively Positive Swab Samples

Alan D. Olstein

^1,*

and

Joellen M. Feirtag

Paradigm Diagnostics, Inc., 800 Transfer Road Suite 12, St. Paul, MN 55114, USA

Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108, USA

Author to whom correspondence should be addressed.

Microorganisms 2019, 7(5), 151; https://doi.org/10.3390/microorganisms7050151

Received: 18 April 2019 / Revised: 20 May 2019 / Accepted: 25 May 2019 / Published: 27 May 2019

(This article belongs to the Special Issue Foodborne Pathogen Distribution, Ecology, Inactivation and Methods of Differentiation)

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Abstract

PDX-LIB, Listeria Indicator Broth, was developed as a proprietary sensitive screening test to identify presumptively positive environmental swab samples for Listeria sp. The original formulation, while sensitive, initially proved to exhibit acceptable levels of false positive test results. Paradigm Diagnostics has been undertaken to modify the medium formulation to render it more selective while not sacrificing its sensitivity. After identification of a candidate formulation through laboratory studies, a field trial was conducted to validate the test performance parameters, including the true positive frequency and false positive frequency in several different food-processing facilities. Identical swab samples were enriched in both the original medium formulation and the new formulation. Presumptive positive samples were confirmed by plating on selective differential agar and qPCR analysis. The field trial data demonstrate that the new formulation significantly reduces the frequency of false positive samples compared to the original Listeria Indicator Broth formulation, without compromising the sensitivity of the original formulation. The new medium formulation resulted in no false positive samples compared to the 54% increased presumptive positive samples obtained with the original medium formulation. View Full-Text

Keywords: food safety; environmental Listeria; Listeria detection

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MDPI and ACS Style

Olstein, A.D.; Feirtag, J.M. Improved Positive Predictive Performance of Listeria Indicator Broth: A Sensitive Environmental Screening Test to Identify Presumptively Positive Swab Samples. Microorganisms 2019, 7, 151. https://doi.org/10.3390/microorganisms7050151

AMA Style

Olstein AD, Feirtag JM. Improved Positive Predictive Performance of Listeria Indicator Broth: A Sensitive Environmental Screening Test to Identify Presumptively Positive Swab Samples. Microorganisms. 2019; 7(5):151. https://doi.org/10.3390/microorganisms7050151

Chicago/Turabian Style

Olstein, Alan D., and Joellen M. Feirtag. 2019. "Improved Positive Predictive Performance of Listeria Indicator Broth: A Sensitive Environmental Screening Test to Identify Presumptively Positive Swab Samples" Microorganisms 7, no. 5: 151. https://doi.org/10.3390/microorganisms7050151

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Improved Positive Predictive Performance of Listeria Indicator Broth: A Sensitive Environmental Screening Test to Identify Presumptively Positive Swab Samples

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