Epstein-Barr Virus BBRF2 Is Required for Maximum Infectivity

Masud, H. M. Abdullah Al; Yanagi, Yusuke; Watanabe, Takahiro; Sato, Yoshitaka; Kimura, Hiroshi; Murata, Takayuki

doi:10.3390/microorganisms7120705

Open AccessArticle

Epstein-Barr Virus BBRF2 Is Required for Maximum Infectivity

by

H. M. Abdullah Al Masud

^1,2

,

Yusuke Yanagi

¹,

Takahiro Watanabe

¹

,

Yoshitaka Sato

¹

,

Hiroshi Kimura

^1,* and

Takayuki Murata

^1,3,*

¹

Department of Virology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan

²

Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chattogram 4331, Bangladesh

³

Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoakev 470-1192, Japan

^*

Authors to whom correspondence should be addressed.

Microorganisms 2019, 7(12), 705; https://doi.org/10.3390/microorganisms7120705

Submission received: 13 November 2019 / Revised: 11 December 2019 / Accepted: 14 December 2019 / Published: 16 December 2019

(This article belongs to the Special Issue Epstein–Barr Virus Infection and Associated Diseases)

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Abstract

Epstein-Barr virus (EBV) is a member of the gammaherpesvirinae, which causes infectious mononucleosis and several types of cancer. BBRF2 is an uncharacterized gene of EBV and is expressed during the lytic phase. To evaluate its function, BBRF2-knockout EBV was prepared using bacterial artificial chromosome (BAC) technology and the CRISPR/Cas9 system. Although viral gene expression, DNA synthesis, and progeny secretion were not affected, the infectivity of progeny viruses was significantly reduced by the disruption of BBRF2. When expressed alone, BBRF2 protein localized to the nucleus and cytoplasm, while the coexpression of an interacting partner, BSRF1, resulted in its relocalization to the cytoplasm. Interestingly, the coexpression of BBRF2 protected BSRF1 from proteasome/ubiquitin-dependent degradation. Therefore, BBRF2, together with BSRF1, augments viral infectivity.

Keywords: EBV; BAC; CRISPR/Cas9; BBRF2; lytic cycle

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MDPI and ACS Style

Masud, H.M.A.A.; Yanagi, Y.; Watanabe, T.; Sato, Y.; Kimura, H.; Murata, T. Epstein-Barr Virus BBRF2 Is Required for Maximum Infectivity. Microorganisms 2019, 7, 705. https://doi.org/10.3390/microorganisms7120705

AMA Style

Masud HMAA, Yanagi Y, Watanabe T, Sato Y, Kimura H, Murata T. Epstein-Barr Virus BBRF2 Is Required for Maximum Infectivity. Microorganisms. 2019; 7(12):705. https://doi.org/10.3390/microorganisms7120705

Chicago/Turabian Style

Masud, H. M. Abdullah Al, Yusuke Yanagi, Takahiro Watanabe, Yoshitaka Sato, Hiroshi Kimura, and Takayuki Murata. 2019. "Epstein-Barr Virus BBRF2 Is Required for Maximum Infectivity" Microorganisms 7, no. 12: 705. https://doi.org/10.3390/microorganisms7120705

APA Style

Masud, H. M. A. A., Yanagi, Y., Watanabe, T., Sato, Y., Kimura, H., & Murata, T. (2019). Epstein-Barr Virus BBRF2 Is Required for Maximum Infectivity. Microorganisms, 7(12), 705. https://doi.org/10.3390/microorganisms7120705

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Epstein-Barr Virus BBRF2 Is Required for Maximum Infectivity

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