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Review

Malaria in North-East India: Importance and Implications in the Era of Elimination

by
Devojit Kumar Sarma
1,
Pradumnya Kishore Mohapatra
2,
Dibya Ranjan Bhattacharyya
2,
Savitha Chellappan
3,
Balasubramani Karuppusamy
4,
Keshab Barman
5,
Nachimuthu Senthil Kumar
6,
Aditya Prasad Dash
7,
Anil Prakash
1,* and
Praveen Balabaskaran Nina
8,*
1
ICMR-National Institute for Research in Environmental Health, Bhopal, Madhya Pradesh 462030, India
2
ICMR-RMRC, NE Region, Dibrugarh 786001, Assam, India
3
NITM (ICMR), Belagavi 590010, India
4
Department of Geography, Central University of Tamil Nadu, Tiruvarur 610005, India
5
State NVBDCP Unit, Directorate of Health Services, Govt. of Assam, Guwahati 781005, Assam, India
6
Department of Biotechnology, Mizoram University, Mizoram 796004, India
7
Central University of Tamil Nadu, Tiruvarur 610005, India
8
Department of Epidemiology and Public Health, Central University of Tamil Nadu, Tiruvarur 610005, India
*
Authors to whom correspondence should be addressed.
Microorganisms 2019, 7(12), 673; https://doi.org/10.3390/microorganisms7120673
Submission received: 7 October 2019 / Revised: 26 November 2019 / Accepted: 29 November 2019 / Published: 10 December 2019
(This article belongs to the Special Issue Emerging Vector Borne Infections: A Novel Threat for Global Health)
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Abstract

:
Worldwide and in India, malaria elimination efforts are being ramped up to eradicate the disease by 2030. Malaria elimination efforts in North-East (NE) India will have a great bearing on the overall efforts to eradicate malaria in the rest of India. The first cases of chloroquine and sulfadoxine-pyrimethamine resistance were reported in NE India, and the source of these drug resistant parasites are most likely from South East Asia (SEA). NE India is the only land route through which the parasites from SEA can enter the Indian mainland. India’s malaria drug policy had to be constantly updated due to the emergence of drug resistant parasites in NE India. Malaria is highly endemic in many parts of NE India, and Plasmodium falciparum is responsible for the majority of the cases. Highly efficient primary vectors and emerging secondary vectors complicate malaria elimination efforts in NE India. Many of the high transmission zones in NE India are tribal belts, and are difficult to access. The review details the malaria epidemiology in seven NE Indian states from 2008 to 2018. In addition, the origin and evolution of resistance to major anti-malarials are discussed. Furthermore, the bionomics of primary vectors and emergence of secondary malaria vectors, and possible strategies to prevent and control malaria in NE are outlined.

1. Introduction

Despite large-scale global efforts to eradicate malaria in the last few years, it still remains a major public health burden with an estimated 219 million cases and 435,000 deaths worldwide in 2017 [1]. Even though, there were an estimated 3 million fewer cases than in 2016 (216 million), no significant progress was made in reducing the global malaria burden during 2015–2017 [1]. It is estimated that India and 15 sub-Saharan African countries were responsible for 80% of the world’s malaria burden [1]. The contribution of India to the world’s malaria cases and deaths is estimated to be 4% [1]. Compared to 2016, India reported a 22% decrease in cases in 2017 [1], and most came from Odisha [2].
Malaria transmission in India occurs pre-dominantly from either P. falciparum (Pf) or P. vivax (Pv) [3], however, the prevalence of these species varies greatly across different regions of India. There has been a steady increase in Pf cases in the last 4 decades, and as of December 2018, Pf was responsible for 48.1% of malaria cases in India [2]. North-East (NE) Indian states along with Odisha, Chattisgarh and Jharkhand contribute most of the Pf cases in the country [2]. NE India alone contributes to 12% of India’s Pf cases [2].
Assam, Arunachal Pradesh, Meghalaya, Mizoram, Nagaland, Sikkim, Manipur and Tripura make up the eight states of NE India. The state of Sikkim is mostly malaria free, and the few cases reported are thought to be imported cases, and hence will not be discussed. As of 2018, the seven NE states account for 15.2% of the total malaria cases in India [2]. In India, it is estimated that 162.5 million people live in high-transmission areas [4], which includes many parts of the NE. The hilly and forested areas of NE India are mostly inhabited by the tribal population, and they are at the highest risk of malaria [5]. The distribution of Plasmodium species varies among NE states. Pf is the predominant species in Assam, Mizoram, Tripura and Meghalaya, while Pv is dominant in Nagaland, Arunachal Pradesh and Manipur. Apart from Pf and Pv, the other human malaria parasites, P. ovale and P. malariae have also been recorded from Assam and Arunachal Pradesh [6,7]. The hot and humid climate aided by the numerous hill streams and its tributaries in these tribal areas support perennial mosquito breeding. The primary malaria vectors in NE India are An. minimus and An. baimaii. An. minimus is part of the Minimus Complex that also includes An. harrisoni and An. yaeyamaensis. In NE India, only An. minimus has been reported so far [8]. An. baimaii is part of the An. dirus Complex that includes 7 other sibling species. An. baimaii is the most prevalent sibling species found in NE along with a focal presence of another sibling species, An. dirus X [9]. Apart from these 2 primary vectors, other Anophelines such as An. annularis, An. phillipinensis/nivipes and An. culicifacies are considered to be secondary vectors of malaria in the region [10]. Due to widespread resistance against both chloroquine (CQ) and sulfadoxine-pyrimethamine (SP), artemisinin-lumefantrine (AL) was introduced as the first line anti-malarial against Pf in NE Indian states in 2013 [11,12]. The salient features pertaining to malaria in NE India are outlined in Table 1.
Despite being a hot bed for malaria transmission, malaria intervention studies in NE India have been very challenging due to inaccessibility, difficult terrains, forested areas, administrative issues in the inter-state and international border areas, frequent flooding, poor roads and inadequate network connectivity [5]. Geography and demography of the region, predominance of Pf over Pv, widespread and early emergence of drug resistance to almost all anti-malarials, and the presence of highly anthropophillic malaria vectors like An. baimaii and An. minimus throughout the region makes it different from the rest of India in terms of malaria control and elimination. In early 2016, the Indian Government announced the national framework for malaria elimination (2016–2030) [13,14]. This is in line with the larger Global Technical Strategy of the World Health Organization (WHO) [15] and Asia Pacific leaders to eliminate malaria by 2030 [16]. Successful malaria intervention strategies in the NE are critical for India’s target to eliminate malaria by 2030.

2. Geography of North-East (NE) States

The NE is largely a mountainous territory with two-thirds of its area occupied by hilly and mountainous terrain with heights ranging from 50 m in Brahmaputra valley to 7000 m above mean sea level in the Himalayan borderland [17]. The states are between 22°00′ to 29°30′ N and 89°40′ to 97°25′ E, and cover an area of 255,128 Km2. The NE shares about 4600 Km of international borders with China (on North), Myanmar (on East), Bangladesh (on South) and Bhutan (On West) (Figure 1). The general climate of the NE is tropical monsoon humid climate. The average temperature during winter is 16 °C, and summer is 30 °C. There is a significant climatic contrast between the valleys and the mountainous region. The NE region receives heavy to very heavy rainfall during the South-West Monsoon season (June to September), and rains peak in June. The average annual rainfall is 2000 mm with local variations (1500 to 12,000 mm) [18]. Except for Assam, more than half the geographical area of all NE states is forest. The NE is also one of the two biodiversity hotspots in India [19]. The total population of the region is 45,161,611 (2011 census) and the distribution is not homogeneous. The most densely populated parts of NE region are the plains of Brahmaputra, Barak rivers in Assam, the Imphal plain in Manipur and the western part of Tripura [20].
Assam, located in the central part of NE is considered to be the gateway to NE India as it connects the NE to the Indian mainland by a 22 km strip, called the ‘Chicken’s Neck’, near Siliguri district in West Bengal. Mizoram, Nagaland and Arunachal Pradesh share their international borders with Myanmar (Burma). South East Asia (SEA) is considered to be the hotspot for the generation of drug-resistant parasites. Myanmar is the only land route through which the drug-resistant parasites of SEA can enter India. Thus Mizoram, Nagaland and Arunachal Pradesh, the states bordering Myanmar are strategically important as they can serve as a conduit for the drug-resistant parasites from Myanmar to rest of India.
The NE is inhabited by many tribal groups with unique cultural, social and occupational behavior which have a direct role in malaria transmission. Based on topography and socio-demographic conditions, malaria in the NE region can be classified as tribal malaria, forest malaria, border malaria, and organized sector malaria [21].

3. Malaria Epidemiology (2008 to 2018)

The NE is co-endemic for both Pf and Pv with a low-to-moderate transmission intensity resulting in intermediate to stable malaria in the whole region [22]. During the eradication era (late 1950s to early 1960s), the NE along with the other states of India witnessed a steady decline in malaria cases. However, the situation reversed and malaria made a comeback in 1966, and peaked in 1976 with 10.2% slide positivity rate (SPR) (Figure 2) (See Box A1 for definition of epidemiological parameters) [21]. The surge in malaria cases was due to the emergence of chloroquine resistance (CQR) in Pf and its widespread therapeutic failure all over the NE. Since then, both the SPR and slide falciparum rate (SFR) of the NE remained more or less constant until 2008 when CQ was discontinued, and was replaced by artemisinin combination therapy (ACT) as the first line of anti-malarial treatment. Prior to 2008, some major peaks were also observed, and were mostly due to the failure of SP, and emergence of multi-drug resistant parasites in the NE (Figure 2) [11].
Malaria epidemiology data (Table 2) obtained from National Vector Borne Disease Control Program (NVBDCP) website from 2008 to 2018 for 7 NE states are shown spatio-temporally in Figure 3. The State Vector Borne Disease Control Program (SVBDCP) unit will collect information of all malaria cases from block primary health centers (PHCs), sub-centers and private hospitals and will send it to NVBDCP, New Delhi, every month. From 2008 to 2012, Assam reported the maximum number of malaria cases, followed by Meghalaya. The maximum number of cases (91,413, Pf-66,557) in the study period was observed in Assam followed by Meghalaya (76,759) in 2009. In Assam, from 2009 onwards, there has been a steady decline in malaria cases, and in 2018, the number of cases recorded were 3816 (Pf-2859), below Tripura (13,079) and Meghalaya (6394). In Assam, from a high of 25,815 Pv cases in 2008, there has been a continuous decrease, and in 2018, only 957 cases were reported. As of 2018, Pf and Pv contribute 74.9 and 25.1% of the cases in Assam. Malaria cases in proportion to population is given in Table 3. Among the NE states, Assam has the highest population density (397 persons/sq km). However, based on the cumulative number of cases during the study period (2008–2018), the number of cases per year (0.11%) is comparatively low to the total population.
Fascinatingly, in Meghalaya, the cases nearly doubled from 39,616 (Pf-36,301) in 2008 to 76,759 in 2009 (Pf-74,251). From a peak of 76,759 cases in 2009, there was a gradual decrease in cases until 2012 (20,834, Pf-19,805). From 2013 to 2015, there was a gradual increase in cases (24,727, Pf-22,885 to 48,603, Pf-43,828), and this was followed by a steady decline until 2018 (6394 cases, Pf-6065). Interestingly, Pv cases gradually dropped from 2008 (3315) until 2012 (1029), and was followed by an increase in cases from 2013 (1842) until 2015 (4775), and again was followed by a steep decline until 2018 (329). In Meghalaya, as of 2018, Pf and Pv contributed 94.8 and 5.2% of the cases. Of the 7 NE states, Meghalaya reported the highest proportion of malaria cases (1.15%/year) to the total population.
In the state of Tripura, there was a steady decline in malaria cases from 2008 (25894, Pf-23,588) to 2013 (7396, Pf-6998). However, there was a huge malaria epidemic in 2014, and there was a 6.9 fold rise in malaria cases (51240, Pf-49,653). Even though there was a gradual decrease from 2015 (32,525, Pf-30,074) to 2017 (7051, Pf-6571), malaria cases almost doubled in 2018 (13,079, Pf-12,600). Pf is the leading cause of malaria in Tripura, and contributes to over 90% of the cases. In 2018, Pv contributed to just 3.6% of the cases. Tripura’s proportion of malaria cases per year to the total population is 0.55%, and it ranks fourth in malaria endemicity in the 11 year study period.
In Mizoram, the cases increased from 2008 (7361, Pf-6172) to 2010 (15,594, Pf-14,664), followed by a steep decline in 2011 (8861, Pf-8373). From 2012, the cases started increasing again, and a peak of 28,593 cases (Pf-24,602) was recorded in 2015. There is a decline from 2016 (7583, Pf-5907) to 2018 (4296, Pf-3937). In Mizoram, Pf predominates, and was responsible for 91.6% of the cases. In Mizoram, the population density is 52 persons per sq km, but the number of cases reported is proportionately very high. Nearly, 1.1 percent of the total population is affected on an average per year, and is the second most malarious state.
The states of Arunachal, Nagaland and Manipur have reported a steady decline in malaria cases from 2009 until 2018, and in 2018, the number of cases reported were 625 (Pf-154, Pv-471), 113 (Pf-24, Pv-89) and 12 (Pf-3, Pv-9) respectively. Fascinatingly, in Arunachal and Nagaland, Pv predominates, and in 2018, Pv contributed 75.3 and 78.7% of cases respectively. In Arunachal, the average number of cases to population is 0.75%, and is the third highest among all NE states. In Manipur, over the years, both Pf and Pv have contributed equally to malaria infections. Manipur has the lowest incidence of malaria as the average number of cases to population is just 0.01%.
Pv was more in Arunachal Pradesh, Nagaland and Manipur, the states that border Myanmar, while Pf predominated in Meghalaya, Tripura, Mizoram and Assam, the states that bordered Bangladesh. A declining trend of malaria was observed in Assam, Arunachal Pradesh, Nagaland and Manipur, the states with higher proportion of Pv cases. A cyclical trend was observed in Meghalaya, Tripura and Mizoram dominated by Pf.

4. Drug Resistance

Historically, SEA has been a hotspot for the generation of drug-resistant parasites. In the last 50 years, SEA has been a fertile ground for developing resistance against pyrimethamine, CQ, SP, quinine, mefloquine, and most worryingly, artemisinins [23]. The only land route through which the parasites of SEA can enter India is through Myanmar. The 4 NE states of India: Mizoram, Manipur, Arunachal and Nagaland share their international borders with Myanmar. Most likely, the drug-resistant parasites originating in SEA would have entered the 4 NE states through Myanmar, and subsequently should have spread to the Indian mainland. CQR originated in the late 1950s in SEA [24], and was first reported in Myanmar (Burma) in 1969. In India, CQR was first reported in the Karbi Anglong district of Assam in 1973 [25]. Following the initial reports of CQR, 13 regional teams were set up in 1978 to monitor anti-malarial resistance through in vivo-efficacy trials [26]. From 1978 to 2007, 54 CQ efficacy studies were carried out in the NE involving 2541 patients, and the treatment failure was 35% (893) [26]. Across India, CQ treatment failure was observed most in NE, and is the probable locus from which CQR would have spread to eastern, followed by western, parts of India [26]. In 1982, a national drug policy was implemented to improve the management of malaria cases, and established SP as the treatment in CQ-resistant regions [26]. In 2005, for CQ treatment failures, artesunate plus SP replaced SP alone as the second-line of treatment, and in regions with documented drug resistance, artesunate plus SP was recommended as the first-line of treatment [26]. In 2007, for regions with documented CQ resistance and high-risk districts, artesunate plus SP was selected as the first-line anti-malarial [26]. From 2010 onwards, artesunate plus SP has been the recommended first-line anti-malarial across India [27]. In 2009, nation-wide sentinel sites were set up to monitor anti-malarial drug resistance [28].
The first report of SP resistance was again in Karbi Anglong district of Assam in 1979 [29]. Drug-resistance studies carried out in Assam during 2006 and 2007 identified 12.6% treatment failure in SP, accompanied by resistant mutations in dhfr and dhps [11]. Artesunate plus SP efficacy studies carried out in three NE states (Mizoram, Tripura and Arunachal Pradesh) in 2012 revealed treatment failure rates of >10%, necessitating a change in drug policy [12]. From 2013 onwards, artesunate plus SP was replaced by AL as the first line anti-malarial in NE states, while in the rest of the country, artesunate plus SP continues to remain as the first line of treatment [11,12]. CQ and SP drug-resistance studies carried out in NE are given in Table 4.
The first therapeutic efficacy study on ACT was carried out by Indian Council of Medical Research (ICMR) Regional Medical Research Centre, NE Region, Dibrugarh during 2004–2006 in 4 sites along the Indo-Myanmar border. This is long before ACT was introduced as a first line anti-malarial for treating Pf cases in NE. The study recorded 95.5% Adequate Clinical and Parasitological Response (ACPR) of ACT with mean parasite clearance time of 55:17±14:26 h. It is significant that 4.5% of the cases failed ACT (late clinical failure: 2.1%; and late parasitological failure: 2.4%) [11]. Later studies have identified the failure in ACT is due to the resistance to the partner drug, SP [11,12]. The genetic determinant of artemisinin (ART) resistance has been linked to mutations in the propeller domain of the kelch protein (Pfkelch13 gene) [30]. Molecular screening of Pfkelch13 by ICMR National Institute of Malaria Research (NIMR) has identified one F446I (n =85) and one R561H (n =24) validated ART resistance marker mutations in the Changlang district of Arunachal Pradesh that borders Myanmar [31,32]. In addition, few non-/synonymous mutations (K189T, A578S, and G533A) have also been reported in NE states [31,32]. Both the K189T and A578S mutations were observed in Lunglei district, Mizoram [31]. However, all the reported mutations were not associated with any decline in the clinical efficacy of ACT [31,32,33].
In vitro ring stage survival assay (RSA) carried out on 12 NE isolates obtained from Assam, Arunachal Pradesh and Tripura found that 8 out of 12 (66%) had a greater than 1% survival rate [34]. One of the NE isolates from Assam carried an A675V mutation, a candidate/associate marker, but was not associated with the slow clearance phenotype [34]. In addition, 8 of the 12 isolates had an Asn-Asn (NN) insertion between codon 142 and 143 in the Pfkelch13 gene. Worryingly, the first report of partial ART resistance has been in the Purulia, Bankura and Kolkata regions of West Bengal [35], a state that lies in close proximity to Assam. The widespread ART in SEA necessitates the continuous monitoring of the Indo-Myanmar border to aid malaria control and prevention strategies.
Doxycycline has been recommended by NVBDCP as a short-term prophylaxis (less than 6 weeks) for selective groups from non-endemic areas such as military, para-military forces and travelers [36]. However it is rarely used, and no resistance has been observed so far.
The summary of key events related to malaria in the NE is given in Figure 4.
Table
Table 4. Drug-resistance studies in NE India.
Table 4. Drug-resistance studies in NE India.
YearAnti-Malarial DrugAssay MethodState/AreaOutcomeRef No.
1973CQTherapeutic efficacyAssamRI level of resistance in 52.5% and RII level in 22.5% of cases*[25]
CQ (1500 mg)28 days in vivoGarohills, MeghalayaRI-33%, RII-9.5%, RIII-12.5%[37]
1974CQTherapeutic efficacyAssamRI-24%[38]
Quinine + pyrimethamine No resistance observed
1975CQ (1500 mg)28 days in vivoBurnihat, MeghalayaRI-78.3%, RII-4.3%[37]
1976CQ (1500 mg)7 days in vivoTezu, Lohit, APRI-17.9%, RII-17.9%, RIII-49.2%
1977CQ (900 mg) + Pyrimethamine (50 mg)7 days in vivoAssam (different districts)0–33.4% resistance[39]
CQ (600 mg) + Pyrimethamine (50 mg)Assam (different districts)0–90.0% resistance
CQ (600 mg) + Pyrimethamine (45 mg)Karbi Anglong, Assam46% resistance
CQ (600 mg) + Pyrimethamine (50 mg)Nagaland51.2% resistance[40]
CQ (900 mg) + Pyrimethamine (50 mg)22% resistance
CQ (600 mg) + Pyrimethamine (45 mg)47% resistance
CQ (1500 mg)Bama Bazar, Garohills, MeghalayaS/RI-82.2%, RIII-18%[37]
CQ (600 mg) + Pyrimethamine (50 mg)Miao, AP29.9% resistance[41]
CQ (600 mg) + Pyrimethamine (50 mg)North TripuraNo resistance observed
CQ (600 mg) + Pyrimethamine (50 mg)Aizawl, Mizoram29% resistance
CQ (600 mg) + Pyrimethamine (50 mg)Jiribam, ManipurNo resistance observed
1978CQ (1500 mg)7 days in vivoKarbi Anglong, Assam66.6% resistance[39]
1979CQ (1500 mg)28 days in vivoMendipathar, Garohills, MeghalayaRI-50%[37]
1995CQ3 days in vivo testAssamS/RI-85%, RI-7%, RII-3%, RIII-5%[42]
Alpha-Beta Arteether28 days therapeutic efficacyDibrugarh, AssamNo resistance observed[43]
1999CQ28 days in vivoJairampur, AP (Indo-Myanmar Border)ETF-60.4%, LTF-22.6%[44]
SPdodoETF-32.6%, LTF-11.6%
quinine7 days in vivodo15.8% treatment failure observed
2001CQ42 days prospective randomized non-blinded trialBokajan, Karbi Anglong, AssamRI-53%, RII-2.2%, RIII-8.9%[45]
SPSonitpur, AssamRI-29%, RII-3.8%, RIII-5.8%
MefloquineRI-4.4%
Mefloquine+artesunateRI-8.7%, RIII-2%
CQRI-37.5%, RII-29%, RIII-29%
SPRI-36.7%, RII-14.3%, RIII-6.1%
MefloquineRI-5.9%, RII-2%
Mefloquine+artesunateRI-1.9%
2002–2003CQ28 days in vivoAPETF-23.8%, LCF-14.3%, LPF-10.7%[46]
SPAPETF-14.1%, LCF-12.6%, LPF-8.1%
2005CQ35 days in vivoAssamRI-13%, RII-4%, RIII-Nil[47]
SP No resistance
2007–2010CQMolecular (Nested PCR)Sonitpur, Assampfcrt K76T-72.13%, pfmdr1 N86Y-41.79%, K76T + N86Y-32.7%[48]
2010CQIn vivo therapeutic efficacyTripura (Indo-Bangladesh Border)~30% ETF, 5% LTF[49]
2011CQ28 days in vivoTripuraETF-32.5%, LCF-35%,[50]
(CQ: Chloroquine, SP: Sulfadoxine/Pyrimethamine, *Classification of anti-malarial treatment types can be found in Box A2).

5. Vectors of NE India

The tropical climate provides a fertile breeding ground for varied mosquito fauna [51]. In India, 61 species of anophelines have been recorded within the subgenera Anopheles and Celia, and there is a possibility of this number increasing with identification of new species under several groups or complexes [52]. Among the 61 Anopheles spp., 9 have been incriminated as malaria vectors in India [53,54]. NE India is part of the “Indo-Burma hot-spot” [55], and is home to diverse fauna and flora. In the early 1990s, Malhotra and Mahanta reported 37 anophelines from NE India [56]. A later survey in early 2000s documented 45 anophelines from this region [P. Dutta, Regional Medical Research Centre, Dibrugarh personal communication]. Anopheles dirus and An. minimus complex mosquitoes are regarded as the main malaria vectors in NE India [8], and these mosquitoes inhabit distinct ecological niches.
The Minimus complex includes three formally named species: An. minimus (earlier species A), An. harrisoni (earlier species C) and An. yaeyamaensis (earlier species E). Present day taxonomy of the An. minimus complex has been resolved by painstaking work of several groups [57,58,59,60]. An. minimus was considered to be the most important malaria vector in the entire sub-Himalayan belt. Due to the large-scale application of dichlorodiphenyltrichloroethane (DDT) under the National Malaria Eradication Program (NMEP), this species was thought to have disappeared from this region. However, it re-emerged in the 1980s and was incriminated as a malaria vector in Assam [61,62], Arunachal Pradesh [63], Nagaland [64] and Mizoram [65]. In NE India, only An. minimus s.s. (species A) has been reported [8]. An. minimus is found throughout the year with higher densities between March and August, and is endophagic, endophillic and highly anthropophillic. It bites humans throughout the night, and the peak biting period is between 01:00 to 04:00AM. It breeds in slow-flowing streams with grassy banks [66], and is susceptible to DDT [67]. An. minimus is a highly efficient malaria vector and is responsible for focal disease outbreaks characterized by high Pf infections and death in forest fringe and foot hills areas of NE. Even though it is susceptible to DDT, behavioural resistance and avoidance of both DDT and long-lasting insecticide nets (LLIN) have been observed [68].
The Minimus and Fluviatilis complexes are phylogenetically [69] and morphologically closely related and are often misidentified [70]. Originally identified as An. fluviatilis in NE India, it is now recognized as a hyper-melanic form of An. minimus (species A) based on molecular data [70]. Both An. minimus and its hyper-melanic form were found in sympatry during winter season, and were also incriminated in malaria transmission [68].
An. baimaii is the widely prevalent species of An. dirus complex mosquitoes in the entire NE, along with a focal presence of An. dirus X (another unnamed species in the complex) in North Cachar [9]. An. baimaii is a forest-dwelling species found in deep forested areas and in forest borders. High anthropophilicity along with its capacity to bite equally both indoor and outdoor, and resting behavior (outside houses) makes it one of the most efficient primary malaria vectors in SEA including NE [71]. Even though the peak biting time of An. baimaii has been estimated to be from 22:00 to 02:00 h, in the NE it has been observed that human feeding starts from 18:00 h, and an estimated 13% of biting occurs in the 1st quarter of the night alone [71,72]. An. baimaii inhabits most of the hilly and forested areas of NE bordering Myanmar and Bangladesh, and most of the drug resistant cases have been recorded from these areas. Literature search shows that CQ resistant Pf has been commonly associated with An. dirus s.l. [65,73,74,75]. In NE, An. baimaii is susceptible to many insecticides. Due to its exophillic nature, conventional use of indoor residual insecticides is ineffective, which is also the major reason for sustained transmission of malaria in An. baimaii-dominated areas. Lack of an effective control strategy against An. baimaii is the major reason for its large population size (Ne), high genetic diversity and lack of population bottleneck in its evolutionary history in the NE [76]. The distribution of primary malaria vectors (An. baimaii and An. minimus) of NE is given in Figure 5. Bionomics of primary malaria vectors are given in Table 5.

6. Role of Secondary Vectors

Apart from these 2 primary vectors, An. phillipinensis, An. nivipes, An. annularis, An. culicifacies and An. vagus have been found to play an important and interesting role in malaria transmission, especially in the winter seasons [77]. Sporadic reports of parasite positivity in these species have been reported in the NE ever since 1941, when Anderson and Viswanathan [78] first incriminated An. culicifacies mosquitoes as a malaria vector in Assam. Similarly, An. phillipinensis has been incriminated as a malaria vector from Burnihut area of Meghalaya during 1968–1969 [79] Since then, many other anophelines have been incriminated as malaria vectors in this region (Table 6), however, their actual role in transmission of malaria is still not clear. The distribution of secondary malaria vectors are shown in Figure 5. Another important aspect of these secondary vectors is that most of them display a wide spectrum of metabolic, physiological and behavioral resistance against many insecticides [80,81,82]. Due to deforestation, shift in agricultural activities, urbanization and climate change, many natural habitats of established malaria vectors are shrinking, and their place has gradually been taken over by other invading species such as An. culicifacies in NE. Therefore, it is important to monitor the vectorial competency of these secondary vectors under changing climate to inform and aid the malaria control program.
Table
Table 5. Bionomics of major malaria vectors in NE India.
Table 5. Bionomics of major malaria vectors in NE India.
SpeciesSibling Species PresentRangeBionomical CharacteristicsDisease Transmission Relationships
Larval HabitatResting HabitatFeeding HabitPeak Biting Activity (hours)Susceptibility Status to InsecticidesDensityInfectivity
An. dirus complexAn. baimaii (Dirus D) [8,9]All NE Indian states except for Sikkim [9]Water pits, elephant footprints, rock bed ravine, mud pools etc.OutdoorHighly anthropophillic, both indoor and outdoor biter [72]21:00–03:00Susceptible [83]5.78# (1986–1988, Arunachal Pradesh) [84]; 0.39* (1989–1990, Assam) [66]; 5.03* (2000–2001, Assam) [85]; 1.54# (2007–2008, Tripura) [49]; 4.0–6.0* (2012, Tripura) [83]; 4.0* (2014, Tripura) [86]0.97% (1986–1988, Arunachal Pradesh) [84]; 1.6% (Aug1995-July1996, Assam) [87]; 3.2% (June 1999 to May 2000, Assam) [88]; 0.7% sporozoite rate (2000, Assam) [85]; 1.9% (1995–2000, Assam) [71]; 11% (2014, Tripura) [86]
An. minimus complexAn. minimus (Sibling Sp. A) [8]All NE Indian states except for SikkimPerennial foothill seepage water streamsHuman-dwellings indoorsHighly anthropophillic01:00-04:00Susceptible [83]1.86# (1986–1988, Arunachal Pradesh) [84]; 0.4* (2014, Tripura) [86]0.40% (1986–1988, Arunachal Pradesh) [84] 0.05% (2012, Tripura) [83].
* Per person per night, #—Trap density per night.
Table
Table 6. Secondary malaria vectors in NE India.
Table 6. Secondary malaria vectors in NE India.
Sl. No.Year of IncriminationMonth/SeasonPlaceSpeciesTotal PositivityMethod of IncriminationReference
11941Not KnownAssamAn. culicifacies0.56%Dissection[78]
21968AugustBurnihat, MeghalayaAn. phillipinensis1 noDissection[79]
31969JulyBurnihat, Meghalaya1 noDissection
42001–2002Throughout the yearJorhat, AssamAn. aconitus3.95%CSP ELISA[77]
An. annularis5.80%
An. hyrcanus gp0.48%
An. jeyporiensis6.25%
An. kochi1.28%
An. phillipinensis/nivipes0.94%
An. tesellatus6.67%
An. varuna3.23%
An. vagus3.87%
52000–2002Throughout the yearJairampur, APAn. Phillipinensis/nivipes1.20%CSP ELISA[89]
Soraipung, DBR, Assam2.60%
Titabor, Assam1.70%
62008–2009April-OctoberJorhat, AssamAn. nivipes0.70%PCR[10]
Dimapur, Nagaland0.95%
72012Pre & Post monsoonAssamAn. culicifacies2.50%CSP ELISA[90]
Meghalaya3.50%
Manipur1.20%
2013Assam10.90%
Meghalaya6.40%
Manipur1.80%
2014Assam6.60%
Meghalaya3.50%
Manipur9.50%
82013March-AugustOrang, AssamAn. vagus0.56*CSP-ICT[91]
Orang, AssamAn. annularis0.22*
Balipara, AssamAn. vagus0.13*
(* Minimum infection rate, CSP: Circumsporozoite protein, ELISA: Enzyme-linked immunosorbant assay, PCR: Polymerase chain reaction, ICT: Immunochromatographic test).

7. Conclusions and Future Directions

Malaria in the NE is highly complex and multi-faceted due to its unique climatic and ecological conditions and, topographic and socio-demographic heterogeneity. In the past, NE has witnessed decline and surge in malaria cases. The significant decline in malaria cases is usually associated with the introduction of new drug or intervention strategies. Also, the emergence of drug resistance, especially to Pf, has led to a resurgence of malaria in this region. First resistance to CQ and SP in India have been reported from NE, and the origin of these resistant parasites can be traced to SEA. With the threat of ART resistance looming in India, it is critical to step up active surveillance in NE states, especially those that border Myanmar. Strict adherence and compliance to the existing drug policy is critical in preventing and controlling drug resistance in the region.
Epidemiological trend indicates that there has been a gradual decrease in cases over the past 11 years in NE. This decline in malaria cases can be attributed to the combined effect of newer and effective drugs (Artemisinin), effective diagnostic system (use of bivalent RDTs) and implementation of effective mosquito control strategies such as LLINs. Even though the cases have decreased, Assam, Arunachal Pradesh, Tripura and Mizoram continue to be malaria-endemic states. As part of the control strategy, high transmission zones or malaria hot-spots in these regions need to be mapped, and have to be continuously monitored. Malaria hotspots from 2013 to 2016 based on annual parasite incidence (API) at district and PHC level in Assam mapped by Geographic Information system (GIS) are shown in Figure 6. Malaria intervention strategies have to be focused on these mapped high-transmission settings. Similar hot spot mapping have to be carried out in other NE states for effective malaria control and elimination in this region.
The true burden of asymptomatic malaria carriers have to be carefully ascertained in these malaria endemic states. Asymptomatic carriers are individuals who carry the malaria parasites without manifesting any clinical symptoms. However, they remain infectious to mosquitoes and continue to spread the disease [92], and they are potential threat to malaria elimination efforts [93]. It is estimated that 20%–70% of the infections cannot be detected by conventional microscopy and RDTs [94]. Active surveillance using molecular tools and treatment of asymptomatic carriers should be given high priority as part of malaria elimination efforts in this region.
Even though Pf is the major cause of malaria in the malaria-endemic states of Tripura, Meghalaya, Assam and Mizoram, a significant number of cases of Pv are reported every year. Furthermore, in the states of Arunachal and Nagaland, Pv is the leading cause of malaria. In these states, Pv cases were recorded throughout the year with a peak during the rainy season and children less than 5 years old were more affected [22]. It is important to understand the relapse pattern and transmission dynamics of Pv in different eco-epidemiological settings for effective control and elimination of Pv in the NE. Also, the status of CQR in Pv has to be continuously monitored in all NE states.
Most of the vector biology studies in the NE have focused around An. baimaii and An. minimus, the major malaria vectors. Climate change, deforestation, urbanization and migration have had a great impact on the current habitat of these established malaria vectors. The natural habitat of the primary vectors are shrinking, and in parallel, this has created habitats for new and emerging malaria vectors. Several studies have reported the emergence of secondary malaria vectors in the NE. The vectorial capacity of other Anophelines in different ecological and epidemiological settings of the NE have to be studied to ascertain their role in malaria epidemiology in this region. An. subpictus incriminated as a major malaria vector in Western India [95] has been identified in mosquito epidemiology studies in Mizoram [96]. Substantial focus has to be given to understanding the emergence of new vectors and vector parasite relationships for formulating effective vector-control strategies in this region.
Overall, malaria control and elimination in the NE will be an important step in India’s efforts to eradicate malaria in the next decade.

Author Contributions

P.B.N., D.K.S., A.P.D. and A.P. designed the review sections and wrote the manuscript. B.K. drew the maps and S.C. collected the data from past studies. P.K.M., D.R.B., K.B., and N.S.K. contributed significantly in the individual sections of the review.

Funding

This research received no external funding

Acknowledgments

The authors acknowledge the contribution of all public health workers and researchers involved in malaria research in the NE. We are also grateful to the work done by NVBDCP, without which this review is not possible.

Conflicts of Interest

There is no conflict of interest among the authors.

Appendix A

Box A1. Definition of epidemiological parameters used in malaria.
Annual parasitic incidence (API): Number of new infections per year per 1000 population (Total no. of positive slides for parasite in a year × 1000/Total population. API - (ABER*SPR)/10
Incidence: Number of newly diagnosed malaria cases during a defined period in a specified population
Malaria mortality rate: Number of deaths from malaria per unit of population during a defined period
Prevalence: Proportion of a specified population with malaria infection at one time
Slide positivity rate (SPR): Proportion of blood smears found to be positive for Plasmodium among all blood smears examined (Total positive × 100/Total slides examined)
Slide falciparum rate (SFR): Proportion of blood smears found to be positive for Pf among all blood smears examined (Total positive Pf 100/Slides examined)
Box A2. Classification of responses to anti-malarial treatment [97].
RI: Recrudescence of the infection between 7 and 28 days after completion of treatment following initial resolution of symptoms and parasite clearance.
RII: Reduction of parasitaemia by >75% at 48 h but failure to clear parasites within 7 days.
RIII: Parasitaemia does not fall by >75% within 48 h
Early treatment failure (ETF):
    Danger signs or severe malaria on day 1, 2 or 3, in the presence of parasitaemia;
    Parasitaemia on day 2 higher than on day 0, irrespective of axillary temperature;
    Parasitaemia on day 3 with axillary temperature ≥37.5 °C; and
    Parasitaemia on day 3 ≥25% of count on day 0.
Late clinical failure (LCF):
    Danger signs or severe malaria in the presence of parasitaemia on any day between day 4 and day 28 (day 42) in patients who did not previously meet any of the criteria of early treatment failure; and
    Presence of parasitaemia on any day between day 4 and day 28 (day 42) with axillary temperature ≥37.5 °C in patients who did not previously meet any of the criteria of early treatment failure.
Late parasitological failure (LPF):
    Presence of parasitaemia on any day between day 7 and day 28 (day 42) with axillary temperature <37.5 °C in patients who did not previously meet any of the criteria of early treatment failure or late clinical failure.
Adequate clinical and parasitological response (ACPR):
    Absence of parasitaemia on day 28 (day 42), irrespective of axillary temperature, in patients who did not previously meet any of the criteria of early treatment failure, late clinical failure or late parasitological failure.

References

  1. WHO. World malaria report; World Health Organization: Geneva, Switzerland, 2018. [Google Scholar]
  2. Available online: https://www.nvbdcp.gov.in/WriteReadData/l892s/95838975781568980798.pdf (accessed on 24 September 2019).
  3. Wangdi, K.; Gatton, M.L.; Kelly, G.C.; Banwell, C.; Dev, V.; Clements, A.C.A. Malaria elimination in India and regional implications. Lancet Infect. Dis. 2016, 16, e214–e224. [Google Scholar] [CrossRef]
  4. Available online: https://www.who.int/malaria/publications/country-profiles/profile_ind_en.pdf?ua=1 (accessed on 24 September 2019).
  5. Dev, V.; Bhattacharyya, P.C.; Talukdar, R. Transmission of malaria and its control in the northeastern region of India. J. Assoc. Physicians India 2003, 51, 1073–1076. [Google Scholar] [PubMed]
  6. Mohapatra, P.K.; Prakash, A.; Bhattacharyya, D.R.; Goswami, B.K.; Ahmed, A.; Sarmah, B.; Mahanta, J. Detection & molecular confirmation of a focus of Plasmodium malariae in Arunachal Pradesh, India. Indian J. Med. Res. 2008, 128, 52–56. [Google Scholar] [PubMed]
  7. Prakash, A.; Mohapatra, P.; Bhattacharyya, D.; Goswami, B.K.; Mahanta, J. Plasmodium ovale: First case report from Assam, India. Curr. Sci. 2003, 84, 1187–1188. [Google Scholar]
  8. Prakash, A.; Walton, C.; Bhattacharyya, D.R.; Loughlin, S.O.; Mohapatra, P.K.; Mahanta, J. Molecular characterization and species identification of the Anopheles dirus and An. minimus complexes in north-east India using r-DNA ITS-2. Acta Trop. 2006, 100, 156–161. [Google Scholar] [CrossRef]
  9. Prakash, A.; Sarma, D.K.; Bhattacharyya, D.R.; Mohapatra, P.K.; Bhattacharjee, K.; Das, K.; Mahanta, J. Spatial distribution and r-DNA second internal transcribed spacer characterization of Anopheles dirus (Diptera: Culicidae) complex species in north-east India. Acta Trop. 2010, 114, 49–54. [Google Scholar] [CrossRef]
  10. Bhattacharyya, D.R.; Prakash, A.; Sarma, N.P.; Mohapatra, P.K.; Singh, S.; Sarma, D.K.; Kalita, M.C.; Mahanta, J. Molecular evidence for the involvement of Anopheles nivipes (Diptera: Culicidae) in the transmission of Plasmodium falciparum in north-eastern India. Ann. Trop. Med. Parasitol. 2010, 104, 331–336. [Google Scholar] [CrossRef]
  11. Mohapatra, P.K.; Sarma, D.K.; Prakash, A.; Bora, K.; Ahmed, M.A.; Sarma, B.; Goswami, B.K.; Bhattacharyya, D.R.; Mahanta, J. Molecular evidence of increased resistance to anti-folate drugs in Plasmodium falciparum in North-East India: A signal for potential failure of artemisinin plus sulphadoxine-pyrimethamine combination therapy. PLoS ONE 2014, 9, e105562. [Google Scholar] [CrossRef] [Green Version]
  12. Mishra, N.; Kaitholia, K.; Srivastava, B.; Shah, N.K.; Narayan, J.P.; Dev, V.; Phookan, S.; Anvikar, A.R.; Rana, R.; Bharti, R.S.; et al. Declining efficacy of artesunate plus sulphadoxine-pyrimethamine in northeastern India. Malar. J. 2014, 13, 284. [Google Scholar] [CrossRef] [Green Version]
  13. WHO-SEARO. India Launches the National Framework to Eliminate Malaria; World Health Organization: Geneva, Switzerland, 2016. [Google Scholar]
  14. Ghosh, S.K.; Rahi, M. Malaria elimination in India-The way forward. J. Vector Borne Dis. 2019, 56, 32–40. [Google Scholar] [CrossRef]
  15. WHO. World Malaria Report; World Health Organization: Geneva, Switzerland, 2015. [Google Scholar]
  16. APMEN. Elimination 2030. Asia Pacific malaria elimination network. 2015. Available online: https://www.apmen.org/web/1/elimination/ (accessed on 24 September 2019).
  17. Dikshit, K.R.; Dikshit, J.K. Relief Features of North-East India. In North-East India: Land, People and Economy; Dikshit, K.R., Dikshit, J.K., Eds.; Springer Netherlands: Dordrecht, The Netherlands, 2014; pp. 91–125. [Google Scholar]
  18. Dikshit, K.R.; Dikshit, J.K. Weather and Climate of North-East India. In North-East India: Land, People and Economy; Springer: Dordrecht, The Netherlands, 2014; pp. 149–173. [Google Scholar]
  19. Dikshit, K.R.; Dikshit, J.K. Natural Vegetation: Forests and Grasslands of North-East India. In North-East India: Land, People and Economy; Springer: Dordrecht, The Netherlands, 2014; pp. 213–255. [Google Scholar]
  20. Dikshit, K.R.; Dikshit, J.K. Population of the North-Eastern States of India. In North-East India: Land, People and Economy; Springer: Dordrecht, The Netherlands, 2014; pp. 421–456. [Google Scholar]
  21. Mohapatra, P.K.; Prakash, A.; Bhattacharyya, D.R.; Mahanta, J. Malaria situation in north-eastern region of India. Icmr Bull. 1998, 28, 21–30. [Google Scholar]
  22. Sharma, V.P.; Dev, V.; Phookan, S. Neglected Plasmodium vivax malaria in northeastern States of India. Indian J. Med. Res. 2015, 141, 546–555. [Google Scholar] [PubMed]
  23. Fairhurst, R.M.; Dondorp, A.M. Artemisinin-Resistant Plasmodium falciparum Malaria. Microbiol. Spectr. 2016, 4, 3. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Payne, D. Spread of chloroquine resistance in Plasmodium falciparum. Parasitol. Today 1987, 3, 241–246. [Google Scholar] [CrossRef]
  25. Sehgal, P.N.; Sharma, M.I.D.; Sharma, S.L.; Gogai, S. Resistance to chloroquine in falciparum malaria in Assam State, India. J. Commun. Dis. 1973, 5, 175–180. [Google Scholar]
  26. Shah, N.K.; Dhillon, G.P.; Dash, A.P.; Arora, U.; Meshnick, S.R.; Valecha, N. Antimalarial drug resistance of Plasmodium falciparum in India: Changes over time and space. Lancet Infect. Dis. 2011, 11, 57–64. [Google Scholar] [CrossRef] [Green Version]
  27. Available online: https://nvbdcp.gov.in/Doc/drug-policy-2010.pdf (accessed on 24 September 2019).
  28. Mishra, N.; Singh, J.P.; Srivastava, B.; Arora, U.; Shah, N.K.; Ghosh, S.K.; Bhatt, R.M.; Sharma, S.K.; Das, M.K.; Kumar, A.; et al. Monitoring antimalarial drug resistance in India via sentinel sites: Outcomes and risk factors for treatment failure, 2009–2010. Bull. World Health Organ. 2012, 90, 895–904. [Google Scholar] [CrossRef]
  29. Das, S.B.B.; Roy, J.R.; Guha, A.K.; Rastogi, A.C. Pyrimethamine in combination with sulfadoxine or sulfalene in P. falciparum infected cases in India. Indian J. Malariol. 1981, 18, 109–116. [Google Scholar]
  30. Ariey, F.; Witkowski, B.; Amaratunga, C.; Beghain, J.; Langlois, A.C.; Khim, N.; Kim, S.; Duru, V.; Bouchier, C.; Ma, L.; et al. A molecular marker of artemisinin-resistant Plasmodium falciparum malaria. Nature 2014, 505, 50–55. [Google Scholar] [CrossRef]
  31. Mishra, N.; Bharti, R.S.; Mallick, P.; Singh, O.P.; Srivastava, B.; Rana, R.; Phookan, S.; Gupta, H.P.; Ringwald, P.; Valecha, N. Emerging polymorphisms in falciparum Kelch 13 gene in Northeastern region of India. Malar. J. 2016, 15, 583. [Google Scholar] [CrossRef] [Green Version]
  32. Mishra, N.; Prajapati, S.K.; Kaitholia, K.; Bharti, R.S.; Srivastava, B.; Phookan, S.; Anvikar, A.R.; Dev, V.; Sonal, G.S.; Dhariwal, A.C.; et al. Surveillance of artemisinin resistance in Plasmodium falciparum in India using the kelch13 molecular marker. Antimicrob. Agents Chemother. 2015, 59, 2548–2553. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Mishra, S.; Bharti, P.K.; Shukla, M.M.; Ali, N.A.; Kashyotia, S.S.; Kumar, A.; Dhariwal, A.C.; Singh, N. Clinical and molecular monitoring of Plasmodium falciparum resistance to antimalarial drug (artesunate+sulphadoxine-pyrimethamine) in two highly malarious district of Madhya Pradesh, Central India from 2012–2014. Pathog. Glob. Health 2017, 111, 186–194. [Google Scholar] [CrossRef] [PubMed]
  34. Chakrabarti, R.; White, J.; Babar, P.H.; Kumar, S.; Mudeppa, D.G.; Mascarenhas, A.; Pereira, L.; Dash, R.; Maki, J.N.; Sharma, A.; et al. Decreased in vitro artemisinin sensitivity of Plasmodium falciparum across India. Antimicrob. Agents Chemother. 2019. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Das, S.; Manna, S.; Saha, B.; Hati, A.K.; Roy, S. Novel pfkelch13 Gene Polymorphism Associates With Artemisinin Resistance in Eastern India. Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am. 2019, 69, 1144–1152. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  36. Anvikar, A. Guidelines for Diagnosis and Treatment of Malaria in India, 2nd ed.; National Institute of Malaria Research: New Delhi, India, 2011. [Google Scholar]
  37. Chakravarty, S.C.; Dwivedi, S.R.; Das, S.; Pukan, D.; Roy, R.G.; Pattanayak, S. Response of Plasmodium falciparum to chloroquine in the Meghalaya state. Indian J. Med. Res. 1979, 70, 34–39. [Google Scholar] [PubMed]
  38. Sehgal, P.N.; Sharma, S.S. Efficacy of quinine with pyrimethamine against chloroquine resistant P. falciparum malaria in Assam State India. J. Commun. Dis. 1974, 6, 175–180. [Google Scholar]
  39. Pattanayak, S.; Roy, R.G.; Phukan, D.; Barkakuty, B.N. Chloroquine resistance in P. falciparum in Assam State. Indian J. Med. Res. 1979, 70, 14–19. [Google Scholar]
  40. Das, S.; Roy, R.G.; Pattanayak, S. A note on chloroquine resistance tests on P. falciparum in Nagaland. Indian J. Med. Res. 1979, 70, 30–33. [Google Scholar]
  41. Pattanayak, S.; Roy, R.G.; Sen, N. Response of Chloroquine with or without pyrimethamine in Plasmodium falciparum in West Bengal, Tripura, Mizoram, Manipur and Arunachal Pradesh. Indian J. Med. Res. 1979, 70, 48–51. [Google Scholar]
  42. Gogoi, S.C.; Dev, V.; Choudhury, B.; Phookan, S. Susceptibility of Plasmodium falciparum to chloroquine in tea garden tribes of Assam, India. Southeast Asian J. Trop. Med. Public Health 1995, 26, 228–230. [Google Scholar]
  43. Mohapatra, P.K.; Khan, A.M.; Prakash, A.; Mahanta, J.; Srivastava, V.K. Effect of arteether alpha/beta on uncomplicated falciparum malaria cases in Upper Assam. Indian J. Med. Res. 1996, 104, 284–287. [Google Scholar] [PubMed]
  44. Mohapatra, P.K.; Namchoom, N.S.; Prakash, A.; Bhattacharya, D.R.; Goswami, B.K.; Mahanta, J. Therapeutic efficacy of anti-malarials in Plasmodium falciparum malaria in an Indo-Myanmar border area of Arunachal Pradesh. Indian J. Med. Res. 2003, 118, 71–76. [Google Scholar] [PubMed]
  45. Campbell, P.; Baruah, S.; Narain, K.; Rogers, C.C. A randomized trial comparing the efficacy of four treatment regimens for uncomplicated falciparum malaria in Assam state, India. Trans. R. Soc. Trop. Med. Hyg. 2006, 100, 108–118. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  46. Mohapatra, P.K.; Prakash, A.; Taison, K.; Negmu, K.; Gohain, A.C.; Namchoom, N.S.; Wange, D.; Bhattacharyya, D.R.; Goswami, B.K.; Borgohain, B.K.; et al. Evaluation of chloroquine (CQ) and sulphadoxine/pyrimethamine (SP) therapy in uncomplicated falciparum malaria in Indo-Myanmar border areas. Trop. Med. Int. Health 2005, 10, 478–483. [Google Scholar] [CrossRef] [PubMed]
  47. Baruah, I.; Talukdar, P.K.; Das, S.C. The drug sensitivities of Plasmodium falciparum in the Sonitpur district, Assam, India. Southeast Asian J. Trop. Med. Public Health 2005, 36, 587–590. [Google Scholar]
  48. Goswami, D.; Dhiman, S.; Rabha, B.; Kumar, D.; Baruah, I.; Veer, V.; Bhola, R.; Sharma, D. High prevalence of pfcrt K76T and mdr1 N86Y mutations in Sonitpur district of Assam, India. J. Parasit. Dis. Off. Organ Indian Soc. Parasitol. 2014, 38, 250–254. [Google Scholar] [CrossRef] [Green Version]
  49. Dhiman, S.; Goswami, D.; Rabha, B.; Gopalakrishnan, R.; Baruah, I.; Singh, L. Malaria epidemiology along Indo-Bangladesh border in Tripura State, India. Southeast Asian J. Trop. Med. Public Health 2010, 41, 1279–1289. [Google Scholar]
  50. Majumdar, T.D.N.; Roy, D.B. Assessment of therapeutic efficacy of chloroquine in uncomplicated Plasmodium falciparum malaria in a rural area of Tripura. J. Pharm. Biomed. Sci. 2011, 10, 1–7. [Google Scholar]
  51. Nagpal, B.N.; Sharma, V.P. Indian anophelines; Science Publishers: Lebanon, NH, USA, 1995. [Google Scholar]
  52. Bhattacharyya, D.; Rajavel, A.; Natarajan, R.; Mohapatra, P.; Jambulingam, P.; Mahanta, J.; Prakash, A. Faunal richness and the checklist of Indian mosquitoes (Diptera: Culicidae). Check List 2014, 10, 1342–1358. [Google Scholar] [CrossRef] [Green Version]
  53. Lal, S.; Sonal, G.S.; Phukar, P.K. Status of malaria in India. J. Indian Acad. Clin. Med. 2000, 5, 19–23. [Google Scholar]
  54. Sharma, V.P. Current scenario of malaria in India. Parassitologia 1999, 41, 349–353. [Google Scholar] [PubMed]
  55. Myers, N.; Mittermeier, R.A.; Mittermeier, C.G.; da Fonseca, G.A.; Kent, J. Biodiversity hotspots for conservation priorities. Nature 2000, 403, 853–858. [Google Scholar] [CrossRef] [PubMed]
  56. Malhotra, P.R.; Mahanta, H.C. Check-List of mosquitoes of Northeast India (Diptera: Culicidae). Orient. Insects 1994, 28, 125–149. [Google Scholar] [CrossRef]
  57. Harbach, R.E.; Parkin, E.; Chen, B.; Butlin, R.K. Anopheles (Cellia) minimus Theobald (Diptera: Culicidae): Neotype designation, characterization, and systematics. Proc. Entomol. Soc. Wash. 2006, 108, 198–209. [Google Scholar]
  58. Harbach, R.E.; Garros, C.; Manh, N.D.; Manguin, S. Formal taxonomy of species C of the Anopheles minimus sibling species complex (Diptera: Culicidae). Zootaxa 2007, 1654, 41–54. [Google Scholar] [CrossRef]
  59. Chen, B.; Harbach, R.E.; Butlin, R.K. Molecular and morphological studies on the Anopheles minimus group of mosquitoes in southern China: Taxonomic review, distribution and malaria vector status. Med. Vet. Entomol. 2002, 16, 253–265. [Google Scholar] [CrossRef]
  60. Somboon, P.; Rory, A.; Tsuda, Y.; Takagi, M.; Harbach, R. Systematics of Anopheles (Cellia) yaeyamaensis sp. n. alias species E of the An. minimus complex in southeastern Asia (Diptera: Culicidae). Zootaxa 2010, 2651, 43–51. [Google Scholar] [CrossRef]
  61. Kareem, M.A.; Singh, Y.K.; Bhatnagar, V.N.; Krishnamurthy, B.S.; Das, M.; Sharma, G.K. A preliminary report on some entomological observations in malaria endemic areas of Kamrup district, Assam. J. Commun. Dis. 1985, 17, 29–35. [Google Scholar]
  62. Prakash, A.; Mohapatra, P.K.; Srivastava, V.K. Vector incrimination in Tamulpur primary health centre, district Nalbari, lower Assam during malaria outbreak 1995. Indian J. Med. Res. 1996, 103, 146–149. [Google Scholar]
  63. Dutta, P.; Baruah, B.D. Incrimination of Anopheles minimus Theobald as a vector of malaria in Arunachal Pradesh. Indian J. Malariol. 1987, 24, 159–162. [Google Scholar]
  64. Bhatnagar, V.N.; Dwivedi, S.R.; Mishra, B.G.; Das, M. Detection and incrimination of Anopheles minimus Theobald 1901 as malaria vector in the foothill areas of Nagaland, India. Indian J. Malariol. 1982, 19, 129–133. [Google Scholar]
  65. Das, S.C.; Baruah, I. Incrimination of Anopheles minimus Theobald and Anopheles balabacensis balabacensis Baisas (A. dirus) as malaria vectors in Mizoram. Indian J. Malariol. 1985, 22, 53–55. [Google Scholar] [PubMed]
  66. Dev, V. Anopheles minimus: Its bionomics and role in the transmission of malaria in Assam, India. Bull. World Health Organ. 1996, 74, 61–66. [Google Scholar] [PubMed]
  67. Prakash, A.; Bhattacharya, D.R.; Mohapatra, P.K.; Mahanta, J. Insecticide susceptibility of Anopheles dirus in Assam. J. Commun. Dis. 1998, 30, 291–292. [Google Scholar]
  68. Dev, V.; Manguin, S. Biology, distribution and control of Anopheles (Cellia) minimus in the context of malaria transmission in northeastern India. Parasites Vectors 2016, 9, 585. [Google Scholar] [CrossRef] [Green Version]
  69. Garros, C.; Harbach, R.E.; Manguin, S. Systematics and biogeographical implications of the phylogenetic relationships between members of the funestus and minimus groups of Anopheles (Diptera: Culicidae). J. Med. Entomol. 2005, 42, 7–18. [Google Scholar] [CrossRef]
  70. Singh, O.P.; Nanda, N.; Dev, V.; Bali, P.; Sohail, M.; Mehrunnisa, A.; Adak, T.; Dash, A.P. Molecular evidence of misidentification of Anopheles minimus as Anopheles fluviatilis in Assam (India). Acta Trop. 2010, 113, 241–244. [Google Scholar] [CrossRef]
  71. Prakash, A.; Bhattacharyya, D.R.; Mohapatra, P.K.; Mahanta, J. Malaria transmission risk by the mosquito Anopheles baimaii (formerly known as An. dirus species D) at different hours of the night in North-east India. Med. Vet. Entomol. 2005, 19, 423–427. [Google Scholar] [CrossRef]
  72. Dutta, P.; Bhattacharyya, D.R.; Khan, S.A.; Sharma, C.K.; Mahanta, J. Feeding patterns of Anopheles dirus, the major vector of forest malaria in north east India. Southeast Asian J. Trop. Med. Public Health 1996, 27, 378–381. [Google Scholar]
  73. Dutta, P.; Bhattacharyya, D.R.; Sharma, C.K.; Dutta, L.P. The importance of Anopheles dirus (A. balabacensis) as a vector of malaria in northeast India. Indian J. Malariol. 1989, 26, 95–101. [Google Scholar]
  74. Gould, D.J.; Cadigan, F.C.; Ward, R.A. Falciparum malaria: Transmission to the gibbon by Anopheles balabacensis. Science 1966, 153, 1384. [Google Scholar] [CrossRef] [PubMed]
  75. Yang, T.H. A review of literature on Anopheles balabacensis balabacensis; WHO/MAL/83.999, WHO/MAL/83.878.30; World Health Organization: Geneva, Switzerland, 1983. [Google Scholar]
  76. Sarma, D.K.; Prakash, A.; O’Loughlin, S.M.; Bhattacharyya, D.R.; Mohapatra, P.K.; Bhattacharjee, K.; Das, K.; Singh, S.; Sarma, N.P.; Ahmed, G.U.; et al. Genetic population structure of the malaria vector Anopheles baimaii in north-east India using mitochondrial DNA. Malar. J. 2012, 11, 76. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  77. Prakash, A.; Bhattacharyya, D.R.; Mohapatra, P.K.; Mahanta, J. Role of the prevalent Anopheles species in the transmission of Plasmodium falciparum and P. vivax in Assam state, north-eastern India. Ann. Trop. Med. Parasitol. 2004, 98, 559–568. [Google Scholar] [CrossRef] [PubMed]
  78. Anderson, L.A.P.; Viswanathan, D.K. The Assam Medical Research Society, Shillong: A resume on its activities during 1931–1941. Calcatta:ThackerSpink Co. 1943. Available online: https://www.cabdirect.org/cabdirect/abstract/19431000207 (accessed on 24 September 2019).
  79. Rajagopal, R. Studies on persistent transmission of malaria in Burnihat, Meghalaya. J. Commun. Dis. 1976, 8, 235–245. [Google Scholar]
  80. Dev, V.; Sharma, V. The Dominant Mosquito Vectors of Human Malaria in India, Anopheles mosquitoes-New insights into malaria vectors, Sylvie Manguin. In Anopheles Mosquitoes, New Insights Into Malaria Vectors; Manguin, S., Ed.; IntechOpen: Rijeka, Croatia, 2013; Available online: https://www.intechopen.com/books/anopheles-mosquitoes-new-insights-into-malaria-vectors/the-dominant-mosquito-vectors-of-human-malaria-in-india (accessed on 24 September 2019).
  81. Dhiman, S.; Rabha, B.; Goswami, D.; Das, N.G.; Baruah, I.; Bhola, R.K.; Veer, V. Insecticide resistance and human blood meal preference of Anopheles annularis in Asom-Meghalaya border area, northeast India. J. Vector Borne Dis. 2014, 51, 133–136. [Google Scholar] [PubMed]
  82. Raghavendra, K.; Velamuri, P.S.; Verma, V.; Elamathi, N.; Barik, T.K.; Bhatt, R.M.; Dash, A.P. Temporo-spatial distribution of insecticide-resistance in Indian malaria vectors in the last quarter-century: Need for regular resistance monitoring and management. J. Vector Borne Dis. 2017, 54, 111–130. [Google Scholar] [PubMed]
  83. Dev, V.; Adak, T.; Singh, O.P.; Nanda, N.; Baidya, B.K. Malaria transmission in Tripura: Disease distribution & determinants. Indian J. Med. Res. 2015, 142, S12–S22. [Google Scholar]
  84. Dutta, P.; Bhattacharyya, D.R.; Sharma, C.K.; Dutta, L.P. Anopheline fauna of parts of Tirap district, Arunachal Pradesh with reference to malaria transmission. Indian J. Med. Res. 1992, 95, 245–249. [Google Scholar]
  85. Prakash, A.; Bhattacharyya, D.R.; Mohapatra, P.K.; Barua, U.; Phukan, A.; Mahanta, J. Malaria control in a forest camp in an oil exploration area of Upper Assam. Natl. Med. J. India 2003, 16, 135–138. [Google Scholar]
  86. Sarmah, N.; Bhowmik, I.; Sarma, D.; Sharma, C.; Medhi, G.; Mohapatra, P.; Mahanta, J.; Bhattacharyya, D. Role of Anopheles baimaii: Potential vector of epidemic outbreak in Tripura, North-east India. J. Glob. Health Rep. 2019, 3. [Google Scholar] [CrossRef]
  87. Prakash, A.; Bhattacharyya, D.R.; Mohapatra, P.K.; Mahanta, J. Seasonal prevalence of Anopheles dirus and malaria transmission in a forest fringed village of Assam, India. Indian J. Malariol. 1997, 34, 117–125. [Google Scholar] [PubMed]
  88. Prakash, A.; Bhattacharyya, D.R.; Mohapatra, P.K.; Mahanta, J. Estimation of vectorial capacity of Anopheles dirus (Diptera: Culicidae) in a forest-fringed village of Assam (India). Vector Borne Zoonotic Dis. 2001, 1, 231–237. [Google Scholar] [CrossRef] [PubMed]
  89. Prakash, A.; Bhattacharyya, D.R.; Mohapatra, P.K.; Mahanta, J. Potential of Anopheles philippinensis-nivipes complex mosquitoes as malaria vector in north-east India. J. Environ. Biol. 2005, 26, 719–723. [Google Scholar] [PubMed]
  90. Akhtar, N.; Nagpal, B.N.; Kapoor, N.; Srivastava, A.; Valecha, N. Role of An. culicifacies as a vector of malaria in changing ecological scenario of Northeastern states of India. J. Vector Borne Dis. 2016, 53, 264–271. [Google Scholar] [PubMed]
  91. Dhiman, S.; Yadav, K.; Rabha, B.; Goswami, D.; Hazarika, S.; Tyagi, V. Evaluation of Insecticides Susceptibility and Malaria Vector Potential of Anopheles annularis s.l. and Anopheles vagus in Assam, India. PLoS ONE 2016, 11, e0151786. [Google Scholar] [CrossRef] [PubMed]
  92. Lindblade, K.A.; Steinhardt, L.; Samuels, A.; Kachur, S.P.; Slutsker, L. The silent threat: Asymptomatic parasitemia and malaria transmission. Expert Rev. Anti-Infect. Ther. 2013, 11, 623–639. [Google Scholar] [CrossRef] [Green Version]
  93. Sattabongkot, J.; Suansomjit, C.; Nguitragool, W.; Sirichaisinthop, J.; Warit, S.; Tiensuwan, M.; Buates, S. Prevalence of asymptomatic Plasmodium infections with sub-microscopic parasite densities in the northwestern border of Thailand: A potential threat to malaria elimination. Malar. J. 2018, 17, 329. [Google Scholar] [CrossRef] [Green Version]
  94. Poirot, E.; Skarbinski, J.; Sinclair, D.; Kachur, S.P.; Slutsker, L.; Hwang, J. Mass drug administration for malaria. Cochrane Database Syst. Rev. 2013, 12, CD008846. Available online: https://pubmed.ncbi.nlm.nih.gov/24318836-mass-drug-administration-for-malaria (accessed on 24 September 2019). [CrossRef]
  95. Kumar, A.; Hosmani, R.; Jadhav, S.; de Sousa, T.; Mohanty, A.; Naik, M.; Shettigar, A.; Kale, S.; Valecha, N.; Chery, L.; et al. Anopheles subpictus carry human malaria parasites in an urban area of Western India and may facilitate perennial malaria transmission. Malar. J. 2016, 15, 124. [Google Scholar] [CrossRef] [Green Version]
  96. Zomuanpuii, R.; Ringngheti, L.; Brindha, S.; Gurusubramanian, G.; Senthil Kumar, N. ITS2 characterization and Anopheles species identification of the subgenus Cellia. Acta Trop. 2013, 125, 309–319. [Google Scholar] [CrossRef]
  97. Rieckmann, K.H. Monitoring the response of malaria infections to treatment. Bull. World Health Organ. 1990, 68, 759–760. [Google Scholar] [PubMed]
Microorganisms 07 00673 g001 550
Figure 1. Location map of North-East (NE) India. Red line shows the boundaries of the seven states of NE India.
Figure 1. Location map of North-East (NE) India. Red line shows the boundaries of the seven states of NE India.
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Figure 2. Slide positivity rate (SPR), slide falciparum rate (SFR) and emergence of drug resistance in NE states during 1961–2018. Adapted from Mohapatra et al., 2014 [11].
Figure 2. Slide positivity rate (SPR), slide falciparum rate (SFR) and emergence of drug resistance in NE states during 1961–2018. Adapted from Mohapatra et al., 2014 [11].
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Figure 3. Spatio-temporal distribution of Pf and Pv cases in NE India during 2008–2018. The background color shows the population density of the states (as per 2011 census). The proportioned pie-chart represents the total number of Pf and Pv cases reported in the states from 2008 to 2018. (NVBDCP data contains only total malaria cases and Pf cases. Pv data was obtained by subtracting Pf cases from total malaria cases).
Figure 3. Spatio-temporal distribution of Pf and Pv cases in NE India during 2008–2018. The background color shows the population density of the states (as per 2011 census). The proportioned pie-chart represents the total number of Pf and Pv cases reported in the states from 2008 to 2018. (NVBDCP data contains only total malaria cases and Pf cases. Pv data was obtained by subtracting Pf cases from total malaria cases).
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Figure 4. Summary of key events related to Malaria prevention and control in the NE (DDT: Dichlorodiphenyltrichloroethane, NMEP: National Malaria Eradication Program, GFATM: Global Fund to Fight AIDS, Tuberculosis and Malaria, LLIN: Long-lasting insecticide nets, ACT: Artemisinin-based combination therapy, RDT: Rapid diagnostic kit, ACT-AL: ACT Artemether-lumefantrine, NFME: National Framework for Malaria Elimination).
Figure 4. Summary of key events related to Malaria prevention and control in the NE (DDT: Dichlorodiphenyltrichloroethane, NMEP: National Malaria Eradication Program, GFATM: Global Fund to Fight AIDS, Tuberculosis and Malaria, LLIN: Long-lasting insecticide nets, ACT: Artemisinin-based combination therapy, RDT: Rapid diagnostic kit, ACT-AL: ACT Artemether-lumefantrine, NFME: National Framework for Malaria Elimination).
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Figure 5. Distribution of primary and secondary malaria vectors in NE India.
Figure 5. Distribution of primary and secondary malaria vectors in NE India.
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Figure 6. District and primary health center (PHC)-level malaria hotspot in Assam from 2013 to 2016. Malaria hotspots were calculated based on the annual parasite incidence (API) of the respective districts and PHCs.
Figure 6. District and primary health center (PHC)-level malaria hotspot in Assam from 2013 to 2016. Malaria hotspots were calculated based on the annual parasite incidence (API) of the respective districts and PHCs.
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Table
Table 1. Salient features of malaria in North-East (NE) India.
Table 1. Salient features of malaria in North-East (NE) India.
NE StatesAssam, Arunachal Pradesh, Mizoram, Tripura, Meghalaya, Nagaland and Manipur
Geographical Area255,128 Km2 (consists of hills, valleys and plains)
National proportion to land cover8%
Total Population 45161611 (2011 census) with an average density of 159 persons/km2
ClimateTropical monsoon humid climate
Contribution to nation’s malaria cases15.2% (in 2018)
Major Plasmodium spp.P. falciparum and P. vivax
Primary malaria vectorsAn. baimaii and An. minimus
Secondary malaria vectorsAn. annularis, An. phillipinensis/nivipes, An. culicifacies etc.
Treatment for malariaP. falciparum—Age-specific artemisinin combination therapy (Artemether-lumefantrine) with primaquine @ 0.75 mg/kg body weight on day 2
P. vivax—chloroquine (25 mg/kg body weight divided over three days) and primaquine (0.25 mg/kg body weight daily for 14 days.)
For mixed infections—Age-specific artemisinin combination therapy (Artemether-lumefantrine) for 3 days + primaquine 0.25 mg per kg body weight daily for 14 days.
Table
Table 2. Distribution of malaria cases in 7 NE states from 2008 to 2018 (Source: National Vector Borne Disease Control Program (NVBDCP), India).
Table 2. Distribution of malaria cases in 7 NE states from 2008 to 2018 (Source: National Vector Borne Disease Control Program (NVBDCP), India).
StatesAssamArunachal PradeshMeghalayaMizoramNagalandManipurTripura
YearNumber of Malaria CasesPlasmodium falciparumPlasmodium vivaxNumber of Malaria CasesPlasmodium falciparumPlasmodium vivaxNumber of Malaria CasesPlasmodium falciparumPlasmodium vivaxNumber of Malaria CasesPlasmodium falciparumPlasmodium vivaxNumber of Malaria CasesPlasmodium falciparumPlasmodium vivaxNumber of Malaria CasesPlasmodium falciparumPlasmodium vivaxNumber of Malaria CasesPlasmodium falciparumPlasmodium vivax
200883,93958,12425,81529,146821920,92739,61636,30133157361617211895078835424370835635225,89423,5882306
200991,41366,55724,85622,066660215,46476,75974,2512508939973872012848928935596106962044924,43022,9521478
201068,35348,33020,02317,944541212,53241,64239,374226815,59414,66493049591877308294748746023,93921,2542685
201147,39734,70712,69013,9504856909425,14324,0181125886183734883363950241371431440014,41713,812605
201229,99920,579942083682789557920,83419,805102998839437446289182120702558317211,56510,915650
201319,54214,969457363982181421724,72722,885184211,74710,340140722855191766120427873966998398
201414,54011,210333060822338374439,16837,149201923,14521,083206219366471289145727351,24049,6531587
201515,55711,675388250881714337448,60343,828477528,59324,602399115275329952161199732,52530,0742451
20167826568621403128895223335,14731,8673280758359071676828316512122586410,54695451001
20175281349417871546488105816,45414,41820365715497474139418820680225870516571480
20183816285995762515447163946065329429639373591132489123913,07912,600479
Table
Table 3. Malaria cases in proportion to population.
Table 3. Malaria cases in proportion to population.
StatePf CasesPv CasesTotal Cases (2008–2018)Density (2011)Population (2011)Average Cases/Year (2008–2018)Average Cases to PopulationTotal Cases to Population
Arunachal Pradesh35,64878,693114,341171,382,61110,394.630.75%8.3%
Assam278,190109,473387,66339731,169,27235,242.090.11%1.2%
Manipur2176221243881222,721,756398.900.01%0.2%
Meghalaya349,96124,526374,4871322,964,00734,044.271.15%12.6%
Mizoram116,87615,301132,177521,091,01412,016.091.10%12.1%
Nagaland960222,26131,8631191,980,6022896.630.15%1.6%
Tripura207,96214,120222,0823503,671,03220,189.270.55%6.0%

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Sarma, D.K.; Mohapatra, P.K.; Bhattacharyya, D.R.; Chellappan, S.; Karuppusamy, B.; Barman, K.; Senthil Kumar, N.; Dash, A.P.; Prakash, A.; Balabaskaran Nina, P. Malaria in North-East India: Importance and Implications in the Era of Elimination. Microorganisms 2019, 7, 673. https://doi.org/10.3390/microorganisms7120673

AMA Style

Sarma DK, Mohapatra PK, Bhattacharyya DR, Chellappan S, Karuppusamy B, Barman K, Senthil Kumar N, Dash AP, Prakash A, Balabaskaran Nina P. Malaria in North-East India: Importance and Implications in the Era of Elimination. Microorganisms. 2019; 7(12):673. https://doi.org/10.3390/microorganisms7120673

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Sarma, Devojit Kumar, Pradumnya Kishore Mohapatra, Dibya Ranjan Bhattacharyya, Savitha Chellappan, Balasubramani Karuppusamy, Keshab Barman, Nachimuthu Senthil Kumar, Aditya Prasad Dash, Anil Prakash, and Praveen Balabaskaran Nina. 2019. "Malaria in North-East India: Importance and Implications in the Era of Elimination" Microorganisms 7, no. 12: 673. https://doi.org/10.3390/microorganisms7120673

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