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Article

Multiple Lineages of Usutu Virus (Flaviviridae, Flavivirus) in Blackbirds (Turdus merula) and Mosquitoes (Culex pipiens, Cx. modestus) in the Czech Republic (2016–2019)

1
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic
2
Department of Virology, Veterinary Research Institute, 62100 Brno, Czech Republic
3
Faculty of Science, University of South Bohemia, 37005 Ceske Budejovice, Czech Republic
4
Department of Parasitology, Faculty of Science, Charles University, 12800 Prague, Czech Republic
5
Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, 61242 Brno, Czech Republic
6
Institute of Vertebrate Biology, Czech Academy of Sciences, 60365 Brno, Czech Republic
7
Centre for Epidemiology and Microbiology, National Institute of Public Health, 10000 Prague, Czech Republic
8
CEITEC, University of Veterinary and Pharmaceutical Sciences, 61242 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(11), 568; https://doi.org/10.3390/microorganisms7110568
Received: 7 October 2019 / Revised: 29 October 2019 / Accepted: 15 November 2019 / Published: 16 November 2019
(This article belongs to the Special Issue Emerging Vector Borne Infections: A Novel Threat for Global Health)
Usutu virus (USUV) is a flavivirus (Flaviviridae: Flavivirus) of an African origin transmitted among its natural hosts (diverse species of birds) by mosquitoes. The virus was introduced multiple times to Europe where it caused mortality of blackbirds (Turdus merula) and certain other susceptible species of birds. In this study, we report detection of USUV RNA in blackbirds, Culex pipiens and Cx. modestus mosquitoes in the Czech Republic, and isolation of 10 new Czech USUV strains from carcasses of blackbirds in cell culture. Multiple lineages (Europe 1, 2 and Africa 3) of USUV were found in blackbirds and mosquitoes in the southeastern part of the country. A single USUV lineage (Europe 3) was found in Prague and was likely associated with increased mortalities in the local blackbird population seen in this area in 2018. USUV genomic RNA (lineage Europe 2) was detected in a pool of Cx. pipiens mosquitoes from South Bohemia (southern part of the country), where no major mortality of birds has been reported so far, and no flavivirus RNA has been found in randomly sampled cadavers of blackbirds. The obtained data contributes to our knowledge about USUV genetic variability, distribution and spread in Central Europe. View Full-Text
Keywords: Usutu virus; blackbird; mosquito; Culex spp. Usutu virus; blackbird; mosquito; Culex spp.
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MDPI and ACS Style

Hönig, V.; Palus, M.; Kaspar, T.; Zemanova, M.; Majerova, K.; Hofmannova, L.; Papezik, P.; Sikutova, S.; Rettich, F.; Hubalek, Z.; Rudolf, I.; Votypka, J.; Modry, D.; Ruzek, D. Multiple Lineages of Usutu Virus (Flaviviridae, Flavivirus) in Blackbirds (Turdus merula) and Mosquitoes (Culex pipiens, Cx. modestus) in the Czech Republic (2016–2019). Microorganisms 2019, 7, 568. https://doi.org/10.3390/microorganisms7110568

AMA Style

Hönig V, Palus M, Kaspar T, Zemanova M, Majerova K, Hofmannova L, Papezik P, Sikutova S, Rettich F, Hubalek Z, Rudolf I, Votypka J, Modry D, Ruzek D. Multiple Lineages of Usutu Virus (Flaviviridae, Flavivirus) in Blackbirds (Turdus merula) and Mosquitoes (Culex pipiens, Cx. modestus) in the Czech Republic (2016–2019). Microorganisms. 2019; 7(11):568. https://doi.org/10.3390/microorganisms7110568

Chicago/Turabian Style

Hönig, Vaclav, Martin Palus, Tomas Kaspar, Marta Zemanova, Karolina Majerova, Lada Hofmannova, Petr Papezik, Silvie Sikutova, Frantisek Rettich, Zdenek Hubalek, Ivo Rudolf, Jan Votypka, David Modry, and Daniel Ruzek. 2019. "Multiple Lineages of Usutu Virus (Flaviviridae, Flavivirus) in Blackbirds (Turdus merula) and Mosquitoes (Culex pipiens, Cx. modestus) in the Czech Republic (2016–2019)" Microorganisms 7, no. 11: 568. https://doi.org/10.3390/microorganisms7110568

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