Asymmetrical Flow Field-Flow Fractionation on Virus and Virus-Like Particle Applications
Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, Viikinkaari 9, University of Helsinki, FI-00014 Helsinki, Finland
*
Authors to whom correspondence should be addressed.
Microorganisms 2019, 7(11), 555; https://doi.org/10.3390/microorganisms7110555
Received: 11 October 2019
/
Revised: 28 October 2019
/
Accepted: 8 November 2019
/
Published: 12 November 2019
(This article belongs to the Section Microbial Biotechnology)
Asymmetrical flow field-flow fractionation (AF4) separates sample components based on their sizes in the absence of a stationary phase. It is well suited for high molecular weight samples such as virus-sized particles. The AF4 experiment can potentially separate molecules within a broad size range (~103−109 Da; particle diameter from 2 nm to 0.5−1 μm). When coupled to light scattering detectors, it enables rapid assays on the size, size distribution, degradation, and aggregation of the studied particle populations. Thus, it can be used to study the quality of purified viruses and virus-like particles. In addition to being an advanced analytical characterization technique, AF4 can be used in a semi-preparative mode. Here, we summarize and provide examples on the steps that need optimization for obtaining good separation with the focus on virus-sized particles.
View Full-Text
Keywords:
virus; virus-like particle; field-flow fractionation; size-based separation; macromolecular complex; virus purification; light scattering
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Eskelin, K.; Poranen, M.M.; Oksanen, H.M. Asymmetrical Flow Field-Flow Fractionation on Virus and Virus-Like Particle Applications. Microorganisms 2019, 7, 555. https://doi.org/10.3390/microorganisms7110555
AMA Style
Eskelin K, Poranen MM, Oksanen HM. Asymmetrical Flow Field-Flow Fractionation on Virus and Virus-Like Particle Applications. Microorganisms. 2019; 7(11):555. https://doi.org/10.3390/microorganisms7110555
Chicago/Turabian StyleEskelin, Katri, Minna M. Poranen, and Hanna M. Oksanen. 2019. "Asymmetrical Flow Field-Flow Fractionation on Virus and Virus-Like Particle Applications" Microorganisms 7, no. 11: 555. https://doi.org/10.3390/microorganisms7110555
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
