Next Article in Journal
Conferring the Metabolic Self-Sufficiency of the CAM Plasmid of Pseudomonas putida ATCC 17453: The Key Role of Putidaredoxin Reductase
Next Article in Special Issue
Taxonomic Characterization, and Secondary Metabolite Analysis of Streptomyces triticiradicis sp. nov.: A Novel Actinomycete with Antifungal Activity
Previous Article in Journal
The Impact of Bioinformatics Pipelines on Microbiota Studies: Does the Analytical “Microscope” Affect the Biological Interpretation?
Previous Article in Special Issue
Characterization of Streptomyces sporangiiformans sp. nov., a Novel Soil Actinomycete with Antibacterial Activity against Ralstonia solanacearum
Open AccessArticle

Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate Streptomyces sp. SM17 When Compared to Its Terrestrial Relative S. albidoflavus J1074

1
School of Microbiology, University College Cork, T12 YN60 Cork, Ireland
2
Marine Biodiscovery, School of Chemistry and Ryan Institute, National University of Ireland Galway (NUI Galway), University Road, H91 TK33 Galway, Ireland
3
Environmental Research Institute, University College Cork, T23 XE10 Cork, Ireland
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(10), 394; https://doi.org/10.3390/microorganisms7100394
Received: 12 August 2019 / Revised: 21 August 2019 / Accepted: 24 September 2019 / Published: 26 September 2019
Much recent interest has arisen in investigating Streptomyces isolates derived from the marine environment in the search for new bioactive compounds, particularly those found in association with marine invertebrates, such as sponges. Among these new compounds recently identified from marine Streptomyces isolates are the octapeptidic surugamides, which have been shown to possess anticancer and antifungal activities. By employing genome mining followed by an one strain many compounds (OSMAC)-based approach, we have identified the previously unreported capability of a marine sponge-derived isolate, namely Streptomyces sp. SM17, to produce surugamide A. Phylogenomics analyses provided novel insights on the distribution and conservation of the surugamides biosynthetic gene cluster (sur BGC) and suggested a closer relatedness between marine-derived sur BGCs than their terrestrially derived counterparts. Subsequent analysis showed differential production of surugamide A when comparing the closely related marine and terrestrial isolates, namely Streptomyces sp. SM17 and Streptomyces albidoflavus J1074. SM17 produced higher levels of surugamide A than S. albidoflavus J1074 under all conditions tested, and in particular producing >13-fold higher levels when grown in YD and 3-fold higher levels in SYP-NaCl medium. In addition, surugamide A production was repressed in TSB and YD medium, suggesting that carbon catabolite repression (CCR) may influence the production of surugamides in these strains. View Full-Text
Keywords: genome mining; OSMAC; phylogenomics; secondary metabolites; surugamides; surugamide A; marine sponge-associated bacteria; Streptomyces; albidoflavus phylogroup genome mining; OSMAC; phylogenomics; secondary metabolites; surugamides; surugamide A; marine sponge-associated bacteria; Streptomyces; albidoflavus phylogroup
Show Figures

Figure 1

MDPI and ACS Style

Almeida, E.L.; Kaur, N.; Jennings, L.K.; Carrillo Rincón, A.F.; Jackson, S.A.; Thomas, O.P.; Dobson, A.D. Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate Streptomyces sp. SM17 When Compared to Its Terrestrial Relative S. albidoflavus J1074. Microorganisms 2019, 7, 394.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop