Next Article in Journal
Modulation of Enterohaemorrhagic Escherichia coli Survival and Virulence in the Human Gastrointestinal Tract
Next Article in Special Issue
Role of Oral Microbiota in Cancer Development
Previous Article in Journal
Enzymes Catalyzing the TCA- and Urea Cycle Influence the Matrix Composition of Biofilms Formed by Methicillin-Resistant Staphylococcus aureus USA300
Article Menu
Issue 4 (December) cover image

Export Article

Open AccessArticle
Microorganisms 2018, 6(4), 114; https://doi.org/10.3390/microorganisms6040114

Role of SCFAs for Fimbrillin-Dependent Biofilm Formation of Actinomyces oris

1
Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan
2
Department of Pediatric Dentistry, Nihon University Graduate School of Dentistry at Matsudo, Chiba 271-8587, Japan
*
Author to whom correspondence should be addressed.
Received: 12 October 2018 / Revised: 8 November 2018 / Accepted: 10 November 2018 / Published: 13 November 2018
(This article belongs to the Special Issue Oral Microbiota in Health and Disease)
Full-Text   |   PDF [3890 KB, uploaded 13 November 2018]   |  

Abstract

Actinomyces oris expresses type 1 and 2 fimbriae on the cell surface. Type 2 fimbriae mediate co-aggregation and biofilm formation and are composed of the shaft fimbrillin FimA and the tip fimbrillin FimB. Short-chain fatty acids (SCFAs) are metabolic products of oral bacteria, but the effects of exogenous SCFAs on FimA-dependent biofilm formation are poorly understood. We performed two types of biofilm formation assays using A. oris MG1 or MG1.ΔfimA to observe the effects of SCFAs on FimA-dependent biofilm formation in 96-well and six-well microtiter plates and a flow cell system. SCFAs did not induce six- and 16-hour biofilm formation of A. oris MG1 and MG1.ΔfimA in saliva-coated 96-well and six-well microtiter plates in which metabolites produced during growth were not excluded. However, 6.25 mM butyric acid and 3.125 mM propionic acid induced FimA-dependent biofilm formation and cell death in a flow cell system in which metabolites produced during growth were excluded. Metabolites produced during growth may lead to disturbing effects of SCFAs on the biofilm formation. The pure effects of SCFAs on biofilm formation were induction of FimA-dependent biofilm formation, but the stress responses from dead cells may regulate its effects. Therefore, SCFA may play a key role in A. oris biofilm formation. View Full-Text
Keywords: biofilm; initial colonization; Actinomyces oris; fimbrillin; SCFAs biofilm; initial colonization; Actinomyces oris; fimbrillin; SCFAs
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Suzuki, I.; Shimizu, T.; Senpuku, H. Role of SCFAs for Fimbrillin-Dependent Biofilm Formation of Actinomyces oris. Microorganisms 2018, 6, 114.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Microorganisms EISSN 2076-2607 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top