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Microorganisms 2018, 6(4), 112; https://doi.org/10.3390/microorganisms6040112

Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestals

Instituto de Biotecnología CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Los Reseros y Nicolás Repetto, Hurlingham Buenos Aires 1686, Argentina
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Received: 18 September 2018 / Revised: 20 October 2018 / Accepted: 24 October 2018 / Published: 27 October 2018
(This article belongs to the Special Issue Pathogenesis of Enterohaemorrhagic Escherichia coli)
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Abstract

Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic Escherichia coli (EPEC) are attaching and effacing (A/E) pathogens, which translocate effector proteins to intestinal enterocytes through a type III secretion system (T3SS). T3SS and most of its effector proteins are encoded in a pathogenicity island called LEE. Recently, new effectors have been located outside the LEE. This study aimed to characterize EspY3, a novel non-LEE encoded T3SS effector of EHEC. EspY3 shares homology with SopD and PipB2 effector proteins of Salmonella’s T3SS-1 and T3SS-2, respectively. The presence of recombinant EspY3 in the supernatant samples demonstrated that EspY3 was secreted by the T3SS of EHEC and EPEC. Through infection assays, we demonstrated the translocation of EspY3 into Caco-2 cells by T3SS of EPEC. The subcellular localization of EspY3 was determined in the pedestal region, where its presence generates a significant increase in the size of the pedestals area. The EspY3 effector induced the elongation of polymerized actin pedestals in infected Caco-2 by EPEC. This study confirmed that EspY3 is part of the repertoire of T3SS effectors of EHEC O157:H7, and that it participates in modeling cellular actin during the infection. View Full-Text
Keywords: pathogenic Escherichia coli; type III secretion system; effectors; actin polymerization; pedestals pathogenic Escherichia coli; type III secretion system; effectors; actin polymerization; pedestals
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Larzábal, M.; Marques Da Silva, W.; Riviere, N.A.; Cataldi, Á.A. Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestals. Microorganisms 2018, 6, 112.

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