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Microorganisms
Volume 14
Issue 9
10.3390/microorganisms14092096
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Article

Whole-Genome Analysis, Biosynthetic Gene Cluster Mining of Amycolatopsis sp. nov. TRM77291 and Antibacterial Activity of Siderochelin A

by
Yi-Huang Chen
,
Lu-Yao Wang
,
Tao Luo
,
Mei-Ting Wang
,
Yu-Xiang Yao
,
Jian-Ming Wang
,
Zhu-Feng Zhang
* and
Xiao-Xia Luo
*
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science, Tarim University, Alar 843300, China
*
Authors to whom correspondence should be addressed.
Microorganisms 2026, 14(9), 2096; https://doi.org/10.3390/microorganisms14092096 (registering DOI)
Submission received: 20 August 2026 / Revised: 8 September 2026 / Accepted: 15 September 2026 / Published: 19 September 2026
(This article belongs to the Special Issue Advances in Antimicrobial Compounds)
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Abstract

The genus Amycolatopsis, belonging to the phylum Actinobacteria, is known for producing diverse bioactive secondary metabolites and plays a significant role in microbial drug discovery. This study centers on the strain TRM77291, sourced from a unique habitat, and is postulated to represent a potential new species. This hypothesis is based on 16S rRNA gene phylogenetic analysis combined with physiological, biochemical, and chemotaxonomic characterization. The strain’s genome, consisting of 8.76 Mb with a G + C content of 68.89% and encoding 8572 genes, was analyzed through whole-genome sequencing. AntiSMASH analysis identified 38 biosynthetic gene clusters related to PKS, NRPS, and RiPPs, while BIG-SCAPE analysis indicated the potential for unique compound synthesis, including Cluster 7 and Cluster 35. Screening ten fermentation media determined millet medium as the most optimal choice. Siderochelin A, a monomeric compound isolated from a fermentation product, demonstrated antibacterial efficacy against various pathogenic bacteria. This study revealed a notable activity of Siderochelin A against Escherichia coli, providing a theoretical basis for discovering novel species, exploring secondary metabolites, and developing innovative antibiotics.
Keywords: actinobacteria; taxonomic identification; secondary metabolites actinobacteria; taxonomic identification; secondary metabolites

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MDPI and ACS Style

Chen, Y.-H.; Wang, L.-Y.; Luo, T.; Wang, M.-T.; Yao, Y.-X.; Wang, J.-M.; Zhang, Z.-F.; Luo, X.-X. Whole-Genome Analysis, Biosynthetic Gene Cluster Mining of Amycolatopsis sp. nov. TRM77291 and Antibacterial Activity of Siderochelin A. Microorganisms 2026, 14, 2096. https://doi.org/10.3390/microorganisms14092096

AMA Style

Chen Y-H, Wang L-Y, Luo T, Wang M-T, Yao Y-X, Wang J-M, Zhang Z-F, Luo X-X. Whole-Genome Analysis, Biosynthetic Gene Cluster Mining of Amycolatopsis sp. nov. TRM77291 and Antibacterial Activity of Siderochelin A. Microorganisms. 2026; 14(9):2096. https://doi.org/10.3390/microorganisms14092096

Chicago/Turabian Style

Chen, Yi-Huang, Lu-Yao Wang, Tao Luo, Mei-Ting Wang, Yu-Xiang Yao, Jian-Ming Wang, Zhu-Feng Zhang, and Xiao-Xia Luo. 2026. "Whole-Genome Analysis, Biosynthetic Gene Cluster Mining of Amycolatopsis sp. nov. TRM77291 and Antibacterial Activity of Siderochelin A" Microorganisms 14, no. 9: 2096. https://doi.org/10.3390/microorganisms14092096

APA Style

Chen, Y.-H., Wang, L.-Y., Luo, T., Wang, M.-T., Yao, Y.-X., Wang, J.-M., Zhang, Z.-F., & Luo, X.-X. (2026). Whole-Genome Analysis, Biosynthetic Gene Cluster Mining of Amycolatopsis sp. nov. TRM77291 and Antibacterial Activity of Siderochelin A. Microorganisms, 14(9), 2096. https://doi.org/10.3390/microorganisms14092096

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