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Volume 14
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10.3390/microorganisms14102276
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Genomic Analysis of an IMP-1-Producing Atlantibacter hermannii Bloodstream Isolate Carrying a Transferable blaIMP-1-Harbouring IncHI2/pST1 Plasmid Related to Historical Enterobacterales Plasmids in Japan

by
Takashi Okanda
1,
Natsuki Yumura
1,
Daishu Fukushima
1,
Niinyo Nakajima
1,
Tetsuo Yamaguchi
1,*,
Yosuke Tanaka
2,
Kenta Katsumata
3,
Kotaro Aoki
4,
Takuro Koshikawa
1,
Tadatomo Oyanagi
5,
Tomonori Takano
6,
Hiroyuki Kunishima
6,
Mitsuo Kaku
6 and
Hiromu Takemura
1
1
Department of Microbiology, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan
2
Department of Clinical Laboratory, St. Marianna University Yokohama Seibu Hospital, 1197-1 Yasashi-cho, Asahi-ku, Yokohama, Kanagawa 241-0811, Japan
3
Department of Gastroenterological and General Surgery, St. Marianna University Yokohama Seibu Hospital, 1197-1 Yasashi-cho, Asahi-ku, Yokohama, Kanagawa 241-0811, Japan
4
Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo 143-8540, Japan
5
Department of Clinical Laboratory Technology, St. Marianna University Hospital, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan
6
Department of Infectious Diseases, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan
*
Author to whom correspondence should be addressed.
Microorganisms 2026, 14(10), 2276; https://doi.org/10.3390/microorganisms14102276
Submission received: 31 July 2026 / Revised: 3 October 2026 / Accepted: 4 October 2026 / Published: 6 October 2026
(This article belongs to the Special Issue Advances in Clinical Infections and Antimicrobial Resistance)
Versions Notes

Abstract

Atlantibacter hermannii is an uncommon member of the Enterobacterales rarely associated with human infection, and genomic data on antimicrobial resistance in this species remain limited. Carbapenemase-producing A. hermannii isolates have rarely been described. We characterized an IMP-1-producing A. hermannii bloodstream isolate recovered in Japan and assessed the structure and transferability of its blaIMP-1-harbouring IncHI2 plasmid. Minimum inhibitory concentrations were determined by broth microdilution. Complete genome sequencing was performed using Oxford Nanopore technology, followed by comparative plasmid analysis and conjugation experiments. The isolate showed reduced susceptibility to carbapenems and resistance to extended-spectrum cephalosporins, whereas aztreonam, aztreonam–avibactam, and cefiderocol retained in vitro activity. Genome sequencing revealed a circular chromosome and two plasmids, including a 289-kb IncHI2 plasmid belonging to plasmid sequence type 1 (pST1) and harbouring blaIMP-1 within a class 1 integron. Comparative analysis showed structural conservation with IncHI2/pST1 plasmids identified in Japanese Enterobacterales isolates in 2007 and 2018. Conjugation experiments confirmed transfer to Escherichia coli, with markedly higher transfer frequencies at 30–35 °C than at 37 °C. These findings provide genomic and functional evidence for the continued detection of closely related blaIMP-1-harbouring IncHI2/pST1 plasmids among different Enterobacterales species in Japan over an extended period.
Keywords: Atlantibacter hermannii; blaIMP-1; IncHI2 plasmid; carbapenemase; integron; conjugation Atlantibacter hermannii; blaIMP-1; IncHI2 plasmid; carbapenemase; integron; conjugation

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MDPI and ACS Style

Okanda, T.; Yumura, N.; Fukushima, D.; Nakajima, N.; Yamaguchi, T.; Tanaka, Y.; Katsumata, K.; Aoki, K.; Koshikawa, T.; Oyanagi, T.; et al. Genomic Analysis of an IMP-1-Producing Atlantibacter hermannii Bloodstream Isolate Carrying a Transferable blaIMP-1-Harbouring IncHI2/pST1 Plasmid Related to Historical Enterobacterales Plasmids in Japan. Microorganisms 2026, 14, 2276. https://doi.org/10.3390/microorganisms14102276

AMA Style

Okanda T, Yumura N, Fukushima D, Nakajima N, Yamaguchi T, Tanaka Y, Katsumata K, Aoki K, Koshikawa T, Oyanagi T, et al. Genomic Analysis of an IMP-1-Producing Atlantibacter hermannii Bloodstream Isolate Carrying a Transferable blaIMP-1-Harbouring IncHI2/pST1 Plasmid Related to Historical Enterobacterales Plasmids in Japan. Microorganisms. 2026; 14(10):2276. https://doi.org/10.3390/microorganisms14102276

Chicago/Turabian Style

Okanda, Takashi, Natsuki Yumura, Daishu Fukushima, Niinyo Nakajima, Tetsuo Yamaguchi, Yosuke Tanaka, Kenta Katsumata, Kotaro Aoki, Takuro Koshikawa, Tadatomo Oyanagi, and et al. 2026. "Genomic Analysis of an IMP-1-Producing Atlantibacter hermannii Bloodstream Isolate Carrying a Transferable blaIMP-1-Harbouring IncHI2/pST1 Plasmid Related to Historical Enterobacterales Plasmids in Japan" Microorganisms 14, no. 10: 2276. https://doi.org/10.3390/microorganisms14102276

APA Style

Okanda, T., Yumura, N., Fukushima, D., Nakajima, N., Yamaguchi, T., Tanaka, Y., Katsumata, K., Aoki, K., Koshikawa, T., Oyanagi, T., Takano, T., Kunishima, H., Kaku, M., & Takemura, H. (2026). Genomic Analysis of an IMP-1-Producing Atlantibacter hermannii Bloodstream Isolate Carrying a Transferable blaIMP-1-Harbouring IncHI2/pST1 Plasmid Related to Historical Enterobacterales Plasmids in Japan. Microorganisms, 14(10), 2276. https://doi.org/10.3390/microorganisms14102276

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