Essential Oils as an Antifungal Alternative for the Control of Various Species of Fungi Isolated from Musa paradisiaca: Part I
Abstract
1. Introduction
2. Materials and Methods
2.1. Isolation and Purification of Microorganisms
2.2. Morphological Identification
2.3. Molecular Identification Through DNA Sequencing
2.4. Ex Vivo Fungal Activity
2.5. In Vitro Antifungal Activity with Essential Oils
3. Results
3.1. Morphological Identification
3.2. Molecular Identification Through DNA Sequencing
3.3. Fungal Activity Ex Vivo
3.4. In Vitro Antifungal Activity with Essential Oils
4. Discussion
4.1. Morphological Identification
4.2. Molecular Identification Through DNA Sequencing
4.3. Ex Vivo Fungal Activity
4.4. In Vitro Antifungal Activity with Essential Oils
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Organism | Fragment | NCBI | % Identity |
---|---|---|---|
Cladosporium cladosporoides | ITS | MW255614.1 | 99.62% |
Lasiodiplodia theobromae | ITS | OR077890.1 | 100.00% |
Colletotrichum musae | ITS | HQ596292.1 | 99.56% |
Fusarium ophioides | ITS | MH412939.1 | 99.78% |
Aspergillus niger | ITS | NR_111348.1 | 99.83% |
MW255614.1 | H1 | OR077890.1 | H2 | MH412939.1 | H3 | HQ596292.1 | H4 | NR_111348.1 | H5 | |
---|---|---|---|---|---|---|---|---|---|---|
MW255614.1 | ID | 0.003 | 6.248 | 6.248 | 6.248 | 6.248 | 6.248 | 6.248 | 7.225 | 7.225 |
H1 | 0.005 | ID | 6.256 | 6.256 | 6.256 | 6.255 | 6.256 | 6.256 | 7.234 | 7.234 |
OR077890.1 | 0.378 | 0.380 | ID | 0.000 | 0.000 | 0.002 | 0.000 | 0.000 | 6.398 | 6.398 |
H2 | 0.378 | 0.380 | 0.000 | ID | 0.000 | 0.002 | 0.000 | 0.000 | 6.398 | 6.398 |
MH412939.1 | 0.378 | 0.380 | 0.000 | 0.000 | ID | 0.002 | 0.000 | 0.000 | 6.398 | 6.398 |
H3 | 0.380 | 0.382 | 0.002 | 0.002 | 0.002 | ID | 0.002 | 0.002 | 6.397 | 6.397 |
HQ596292.1 | 0.378 | 0.380 | 0.000 | 0.000 | 0.000 | 0.002 | ID | 0.000 | 6.398 | 6.398 |
H4 | 0.378 | 0.380 | 0.000 | 0.000 | 0.000 | 0.002 | 0.000 | ID | 6.398 | 6.398 |
NR_111348.1 | 0.460 | 0.462 | 0.417 | 0.417 | 0.417 | 0.421 | 0.417 | 0.417 | ID | 0.000 |
H5 | 0.460 | 0.462 | 0.417 | 0.417 | 0.417 | 0.421 | 0.417 | 0.417 | 0.000 | ID |
Essential Oil | Fungus | Concentration [ppm] | ||||
---|---|---|---|---|---|---|
200 | 400 | 600 | 800 | 1000 | ||
Cinnamon | Cladosporium spp. | + | − | − | − | − |
Lasiodiplodia spp. | − | − | − | − | − | |
Colletotrichum spp. | − | − | − | − | − | |
Fusarium spp. | − | − | − | − | − | |
Aspergillus spp. | − | − | − | − | − | |
Clove | Cladosporium spp. | + | − | − | − | − |
Lasiodiplodia spp. | + | + | + | + | − | |
Colletotrichum spp. | + | − | − | − | − | |
Fusarium spp. | + | + | + | − | − | |
Aspergillus spp. | + | + | − | − | − | |
Basil | Cladosporium spp. | + | + | − | − | − |
Lasiodiplodia spp. | + | + | + | + | + | |
Colletotrichum spp. | + | + | + | + | + | |
Fusarium spp. | + | + | + | + | + | |
Aspergillus spp. | + | + | − | − | − | |
Oregano | Cladosporium spp. | − | − | − | − | − |
Lasiodiplodia spp. | − | − | − | − | − | |
Colletotrichum spp. | − | − | − | − | − | |
Fusarium spp. | + | + | + | − | − | |
Aspergillus spp. | − | − | − | − | − | |
Rosemary | Cladosporium spp. | + | + | + | − | − |
Lasiodiplodia spp. | + | + | + | + | + | |
Colletotrichum spp. | + | + | + | + | + | |
Fusarium spp. | + | + | + | + | + | |
Aspergillus spp. | + | + | + | + | + | |
Thyme | Cladosporium spp. | − | − | − | − | − |
Lasiodiplodia spp. | + | + | − | − | − | |
Colletotrichum spp. | − | − | − | − | − | |
Fusarium spp. | − | − | − | − | − | |
Aspergillus spp. | + | + | − | − | − |
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Ruiz Medina, M.D.; Ruales, J. Essential Oils as an Antifungal Alternative for the Control of Various Species of Fungi Isolated from Musa paradisiaca: Part I. Microorganisms 2025, 13, 1827. https://doi.org/10.3390/microorganisms13081827
Ruiz Medina MD, Ruales J. Essential Oils as an Antifungal Alternative for the Control of Various Species of Fungi Isolated from Musa paradisiaca: Part I. Microorganisms. 2025; 13(8):1827. https://doi.org/10.3390/microorganisms13081827
Chicago/Turabian StyleRuiz Medina, Maritza D., and Jenny Ruales. 2025. "Essential Oils as an Antifungal Alternative for the Control of Various Species of Fungi Isolated from Musa paradisiaca: Part I" Microorganisms 13, no. 8: 1827. https://doi.org/10.3390/microorganisms13081827
APA StyleRuiz Medina, M. D., & Ruales, J. (2025). Essential Oils as an Antifungal Alternative for the Control of Various Species of Fungi Isolated from Musa paradisiaca: Part I. Microorganisms, 13(8), 1827. https://doi.org/10.3390/microorganisms13081827