Next Article in Journal
Enhancing Wastewater Treatment with Aerobic Granular Sludge: Impacts of Tetracycline Pressure on Microbial Dynamics and Structural Stability
Next Article in Special Issue
Characterization of Broad Spectrum Bacteriophage vB ESM-pEJ01 and Its Antimicrobial Efficacy Against Shiga Toxin-Producing Escherichia coli in Green Juice
Previous Article in Journal
L-Rhamnose Globally Changes the Transcriptome of Planktonic and Biofilm Escherichia coli Cells and Modulates Biofilm Growth
Previous Article in Special Issue
Acid Adaptation Enhances Tolerance of Escherichia coli O157:H7 to High Voltage Atmospheric Cold Plasma in Raw Pineapple Juice
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microorganisms
Volume 12
Issue 9
10.3390/microorganisms12091912
microorganisms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Sequence Types and Antimicrobial Resistance Profiles of Salmonella Typhimurium in the Food Chain in Singapore

by
Yen Ching Lim
1,
Kar Hui Ong
1,
Wei Ching Khor
1,
Favian Yue Xuan Chua
1,
Jia Qi Lim
1,
Li Kiang Tan
1,
Swaine L. Chen
2,3,
Wai Kwan Wong
4,
Matthias Maiwald
5,6,7,
Timothy Barkham
8,
Tse Hsien Koh
7,9,
Joanna Khoo
1,
Joanne Sheot Harn Chan
1,10 and
Kyaw Thu Aung
1,10,11,*
1
National Centre for Food Science, Singapore Food Agency, 7 International Business Park, Singapore 609919, Singapore
2
Infectious Diseases Translational Research Programme, Department of Medicine, Division of Infectious Diseases, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Road, NUHS Tower Block, Singapore 119228, Singapore
3
Laboratory of Bacterial Genomics, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore
4
Centre for Animal & Veterinary Service, National Parks Board, Singapore 718827, Singapore
5
Department of Pathology and Laboratory Medicine, KK Women’s and Children’s Hospital, Singapore 229899, Singapore
6
Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545, Singapore
7
Duke-NUS Medical School, National University of Singapore, Singapore 169857, Singapore
8
Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore 308433, Singapore
9
Department of Microbiology, Singapore General Hospital, Singapore 169856, Singapore
10
Department of Food Science & Technology, National University of Singapore, Science Drive 2, Singapore 117542, Singapore
11
School of Biological Sciences, Nanyang Technological University, 60 Nanyang Dr, Singapore 637551, Singapore
 
add Show full affiliation list
 
remove Hide full affiliation list
*
Author to whom correspondence should be addressed.
Microorganisms 2024, 12(9), 1912; https://doi.org/10.3390/microorganisms12091912
Submission received: 2 September 2024 / Revised: 9 September 2024 / Accepted: 14 September 2024 / Published: 19 September 2024
(This article belongs to the Special Issue Persistence and Antimicrobial Resistance of Bacterial Pathogens in the Food Processing Environment)
Browse Figures
Versions Notes

Abstract

Salmonella remains a significant foodborne pathogen globally with S. Typhimurium presenting as a frequently occurring serovar. This study aimed to characterize 67 S. Typhimurium isolates from humans, food, farms, and slaughterhouses collected in Singapore from 2016 to 2017. Using whole-genome sequencing analysis, the isolates were found to belong to either ST19 (n = 33) or ST36 (n = 34). ST36 predominated in human intestinal and chicken isolates, while human extra-intestinal and non-chicken food isolates belonged to ST19. Plasmids were predicted in 88.1% (n = 59) of the isolates with the most common incompatibility group profiles being IncFIB(S), IncFII(S) and IncQ1. IncFIB(S) (adjusted p-value < 0.05) and IncFII(S) (adjusted p-value < 0.05) were significantly more prevalent in ST19 isolates, while Col156 (adjusted p-value < 0.05) was more significantly found in ST36 isolates. ST36 isolates exhibited higher resistance to multiple antibiotic classes such as penicillins, phenicols, folate pathway inhibitors, aminoglycosides, β-lactam/β-lactamase inhibitor combinations, tetracyclines, and fluoroquinolones. Phylogenetics analysis suggested potential shared routes of transmission among human, chicken, farm and slaughterhouse environments. Taken together, this study offers a cross-sectional epidemiological insight into the genomic epidemiology and antimicrobial landscape of S. Typhimurium isolates in Singapore, informing strategies for future public health and food safety surveillance.
Keywords: Salmonella Typhimurium; antimicrobial resistance; salmonellosis; surveillance; food; human; One Health Salmonella Typhimurium; antimicrobial resistance; salmonellosis; surveillance; food; human; One Health

Share and Cite

MDPI and ACS Style

Lim, Y.C.; Ong, K.H.; Khor, W.C.; Chua, F.Y.X.; Lim, J.Q.; Tan, L.K.; Chen, S.L.; Wong, W.K.; Maiwald, M.; Barkham, T.; et al. Sequence Types and Antimicrobial Resistance Profiles of Salmonella Typhimurium in the Food Chain in Singapore. Microorganisms 2024, 12, 1912. https://doi.org/10.3390/microorganisms12091912

AMA Style

Lim YC, Ong KH, Khor WC, Chua FYX, Lim JQ, Tan LK, Chen SL, Wong WK, Maiwald M, Barkham T, et al. Sequence Types and Antimicrobial Resistance Profiles of Salmonella Typhimurium in the Food Chain in Singapore. Microorganisms. 2024; 12(9):1912. https://doi.org/10.3390/microorganisms12091912

Chicago/Turabian Style

Lim, Yen Ching, Kar Hui Ong, Wei Ching Khor, Favian Yue Xuan Chua, Jia Qi Lim, Li Kiang Tan, Swaine L. Chen, Wai Kwan Wong, Matthias Maiwald, Timothy Barkham, and et al. 2024. "Sequence Types and Antimicrobial Resistance Profiles of Salmonella Typhimurium in the Food Chain in Singapore" Microorganisms 12, no. 9: 1912. https://doi.org/10.3390/microorganisms12091912

APA Style

Lim, Y. C., Ong, K. H., Khor, W. C., Chua, F. Y. X., Lim, J. Q., Tan, L. K., Chen, S. L., Wong, W. K., Maiwald, M., Barkham, T., Koh, T. H., Khoo, J., Chan, J. S. H., & Aung, K. T. (2024). Sequence Types and Antimicrobial Resistance Profiles of Salmonella Typhimurium in the Food Chain in Singapore. Microorganisms, 12(9), 1912. https://doi.org/10.3390/microorganisms12091912

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop