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Volume 11
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10.3390/microorganisms11092361
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Article

Transcriptome and Metabolome Analyses of Thitarodes xiaojinensis in Response to Ophiocordyceps sinensis Infection

by
Miaomiao Li
1,2,
Jihong Zhang
2,
Qilian Qin
2,
Huan Zhang
2,
Xuan Li
2,
Hongtuo Wang
2 and
Qian Meng
2,*
1
College of Basic Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China
2
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
*
Author to whom correspondence should be addressed.
Microorganisms 2023, 11(9), 2361; https://doi.org/10.3390/microorganisms11092361
Submission received: 25 July 2023 / Revised: 18 September 2023 / Accepted: 19 September 2023 / Published: 21 September 2023
(This article belongs to the Special Issue Fungal Biology and Interactions)
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Abstract

Ophiocordyceps sinensis exhibits more than 5 months of vegetative growth in Thitarodes xiaojinensis hemocoel. The peculiar development process of O. sinensis has been elucidated through morphological observation and omics technology; however, little information has been reported regarding the changes that occur in the host T. xiaojinensis. The RNA sequencing data showed that when O. sinensis blastospores were in the proliferative stage, the greatest change in the infected larval fat body was the selectively upregulated immune recognition and antimicrobial peptide genes. When O. sinensis blastospores were in the stationary stage, the immune pathways of T. xiaojinensis reverted to normal levels, which coincides with the successful settlement of O. sinensis. Pathway enrichment analysis showed a higher expression of genes involved in energy metabolism pathway in this stage. Metabolomic analyses revealed a reduction of amino acids and lipids in hemolymph, but an upregulation of lipids in the fat body of the host larvae after O. sinensis infection. We present the first transcriptome integrated with the metabolome study of T. xiaojinensis infected by O. sinensis. It will improve our understanding of the interaction mechanisms between the host and entomopathogenic fungi, and facilitate future functional studies of genes and pathways involved in these interactions.
Keywords: integrated omics analysis; fungus–host interaction; immune defense; lipid metabolism; amino acid integrated omics analysis; fungus–host interaction; immune defense; lipid metabolism; amino acid

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MDPI and ACS Style

Li, M.; Zhang, J.; Qin, Q.; Zhang, H.; Li, X.; Wang, H.; Meng, Q. Transcriptome and Metabolome Analyses of Thitarodes xiaojinensis in Response to Ophiocordyceps sinensis Infection. Microorganisms 2023, 11, 2361. https://doi.org/10.3390/microorganisms11092361

AMA Style

Li M, Zhang J, Qin Q, Zhang H, Li X, Wang H, Meng Q. Transcriptome and Metabolome Analyses of Thitarodes xiaojinensis in Response to Ophiocordyceps sinensis Infection. Microorganisms. 2023; 11(9):2361. https://doi.org/10.3390/microorganisms11092361

Chicago/Turabian Style

Li, Miaomiao, Jihong Zhang, Qilian Qin, Huan Zhang, Xuan Li, Hongtuo Wang, and Qian Meng. 2023. "Transcriptome and Metabolome Analyses of Thitarodes xiaojinensis in Response to Ophiocordyceps sinensis Infection" Microorganisms 11, no. 9: 2361. https://doi.org/10.3390/microorganisms11092361

APA Style

Li, M., Zhang, J., Qin, Q., Zhang, H., Li, X., Wang, H., & Meng, Q. (2023). Transcriptome and Metabolome Analyses of Thitarodes xiaojinensis in Response to Ophiocordyceps sinensis Infection. Microorganisms, 11(9), 2361. https://doi.org/10.3390/microorganisms11092361

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