Impacts of Temperature and Water Activity Interactions on Growth, Aflatoxin B1 Production and Expression of Major Biosynthetic Genes of AFB1 in Aspergillus flavus Isolates
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Isolates
2.2. Fungal Isolates and Growth Conditions
2.3. Growth Assessment
2.4. Quantification of AFB1 by HPLC
2.5. Extraction of RNA, cDNA Synthesis and the RT-PCR Reaction
2.6. Statistical Analysis
3. Results
3.1. Effect of aw × Temperature Interactions on Three Isolates of A. flavus Grown on YES Agar Media
3.2. Effect of aw × Temperature Interactions on AB1 Production by Three A. flavus Isolates on YES Agar Media
3.3. Effect of aw × Temperature Interactions on the Expression of 15 Genes Involved in the Biosynthesis of AFB1 Production
4. Discussion
5. Conclusions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genes | Length (bp) | Primer Sequence |
---|---|---|
aflR | 88 | F: CCTTTCTCACTACTCGGGTTT R: GCAGGTAATCAATAATGTCGG |
aflS | 109 | F: CTCGATGCGGCAGTGTATCT R: ACACCTCCACATGAGCCTTG |
aflA | 111 | F: CATGCTGTTAACCCCCGACT R: AATTGGGCTAGGAAACCGGG |
aflC | 61 | F: TGCATGGCGATGTGGTAGTT R: GTAAGGCCGCGGAAGAAAG |
aflD | 106 | F: ATGCTCCCGTCCTACTGTTT R: ATGTTGGTGATGGTGCTGAT |
aflE | 134 | F: GTGTGGAGGAAGTGATGCGA R: CGGGGTAAGTCCGTTAGCTC |
aflJ | 146 | F: GCGTGATCAGTCGTCAATGC R: CAGGATGAGCGGTTGGTTCT |
aflK | 114 | F: GCTGGGCATTCCAGTACGAT R: CCCATCAACTGACTGTGGCT |
aflM | 100 | F: GAGCCAAAGTCGTGGTGAAC R: GCCTGGATTGCGATAGCGTC |
aflP | 109 | F: CACGCTTTCAGAGCAGGTAA R: TTCGGTGGAGGAGGGAGTT |
aflO | 115 | F: GTCCCGTTTCCTGGGTTGAT R: GCTTTCGATTGCTGCCCAAA |
aflQ | 134 | F: GCACCAACAATTCGGCTCTG R: TGTGGAAGGGTGGAAGATGC |
aflX | 112 | F: AGTCCTCAACATAGCCGCTG R: TAGTCCCCCAGGTTTGACGA |
abaA | 120 | F: ACTGGCAAAAGGAGGTCGAG R: ATTCGAACGGTCTGCTGGTT |
brlA | 131 | F: TCTAGCGGGGATGACCTCAA R: CCGAAGGAAGCCAAAAGTGC |
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Al-Zaban, M.I. Impacts of Temperature and Water Activity Interactions on Growth, Aflatoxin B1 Production and Expression of Major Biosynthetic Genes of AFB1 in Aspergillus flavus Isolates. Microorganisms 2023, 11, 1199. https://doi.org/10.3390/microorganisms11051199
Al-Zaban MI. Impacts of Temperature and Water Activity Interactions on Growth, Aflatoxin B1 Production and Expression of Major Biosynthetic Genes of AFB1 in Aspergillus flavus Isolates. Microorganisms. 2023; 11(5):1199. https://doi.org/10.3390/microorganisms11051199
Chicago/Turabian StyleAl-Zaban, Mayasar I. 2023. "Impacts of Temperature and Water Activity Interactions on Growth, Aflatoxin B1 Production and Expression of Major Biosynthetic Genes of AFB1 in Aspergillus flavus Isolates" Microorganisms 11, no. 5: 1199. https://doi.org/10.3390/microorganisms11051199