Association Studies on Gut and Lung Microbiomes in Patients with Lung Adenocarcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Cohort and Sample Collection
2.2. 16S rRNA Gene Amplicon Sequencing
2.3. Shotgun Metagenome Sequencing
2.4. Phylogenetic Analysis between Gut and Lung Microbiomes
2.5. Statistical Analysis
3. Results
3.1. Characteristics of Study Participants
3.2. The Gut Microbiota of Patients with LADC Exhibited Reduced α-Diversity and Altered Bacterial Composition
3.3. Bacterial Species Found in Both Gut and Lung Microbiota of Patients with LADC
3.4. Comparative Analysis of Metabolic Pathways of Gut Microbiota in Patients with LADC
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Characteristic | Total | LADC | Healthy Controls | p Value * |
---|---|---|---|---|
(n = 107) | (n = 43) | (n = 64) | ||
Sample type | ||||
Feces | 107 | 43 | 64 | |
BALF | 42 | 42 | NA | |
Sex | 0.5538 | |||
Female | 50 (46.7) | 22 (51.2) | 28 (43.8) | |
Male | 57 (53.3) | 21 (48.8) | 36 (56.2) | |
Age (years) | <0.001 | |||
Mean (±SD) | 50 (±13) | 57 (±11) | 46 (±12) | |
Smoking status | 0.8345 | |||
Current or recent smoker | 34 (31.8) | 13 (30.2) | 21 (32.8) | |
Never smoked | 73 (68.2) | 30 (69.8) | 43 (67.2) | |
Disease stage | ||||
I | 20 (46.5) | 20 (46.5) | NA | |
II | 3 (7.0) | 3 (7.0) | NA | |
III | 10 (23.3) | 10 (23.3) | NA | |
IV | 10 (23.3) | 10 (23.3) | NA | |
Distant metastasis | ||||
Absence | 33 (76.7) | 33 (76.7) | NA | |
Presence | 10 (23.3) | 10 (23.3) | NA |
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Guo, Y.; Yuan, W.; Lyu, N.; Pan, Y.; Cao, X.; Wang, Y.; Han, Y.; Zhu, B. Association Studies on Gut and Lung Microbiomes in Patients with Lung Adenocarcinoma. Microorganisms 2023, 11, 546. https://doi.org/10.3390/microorganisms11030546
Guo Y, Yuan W, Lyu N, Pan Y, Cao X, Wang Y, Han Y, Zhu B. Association Studies on Gut and Lung Microbiomes in Patients with Lung Adenocarcinoma. Microorganisms. 2023; 11(3):546. https://doi.org/10.3390/microorganisms11030546
Chicago/Turabian StyleGuo, Yaqiong, Wenjie Yuan, Na Lyu, Yuanlong Pan, Xiaoqing Cao, Yuxuan Wang, Yi Han, and Baoli Zhu. 2023. "Association Studies on Gut and Lung Microbiomes in Patients with Lung Adenocarcinoma" Microorganisms 11, no. 3: 546. https://doi.org/10.3390/microorganisms11030546
APA StyleGuo, Y., Yuan, W., Lyu, N., Pan, Y., Cao, X., Wang, Y., Han, Y., & Zhu, B. (2023). Association Studies on Gut and Lung Microbiomes in Patients with Lung Adenocarcinoma. Microorganisms, 11(3), 546. https://doi.org/10.3390/microorganisms11030546