The Archaeome’s Role in Colorectal Cancer: Unveiling the DPANN Group and Investigating Archaeal Functional Signatures
Abstract
:1. Introduction
2. Materials and Methods
2.1. Published Fecal Metagenomic Data
2.2. Bioinformatic Processing and Biostatistical Analysis
2.2.1. Taxonomic Analysis
Archaea Identification
Data Filtering and Archaeal Species Patterns in Colorectal Cancer
Correlation Analysis of DPANN Archaea and Other Archaeal Species
Multivariate Analysis
2.2.2. Data Assembly
2.2.3. Functional Analysis
Genome Annotation
Association of Archaeal Genes with Colorectal Cancer
3. Results
3.1. Study Characterization and Data Processing
3.2. Archaeal Communities Associated with CRC Microbiomes
3.3. DPANN Archaeon Association with the Different Archaeal Species Detected in Healthy and CRC Subjects
3.4. Archaeal Proteins Identified in the Gut Microbiomes of Healthy Individuals and CRC Patients
3.5. Association of Proteins Exclusively Detected in Archaea with Colorectal Cancer
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Project n° | CRC | Healthy | Country | Reference |
---|---|---|---|---|
PRJEB7774 | 20 | 24 | Austria | [29] |
PRJEB27928 | 42 | 39 | Germany | [30] |
PRJNA447983 | 22 | 22 | Italy | [31] |
PRJDB4176 | 33 | 35 | Japan | [32] |
PRJEB10878 | 46 | 48 | China | [33] |
PRJNA397112 | 29 | 30 | India | [34] |
Phylum | Spearman’s Correlation Coefficient (r) | p-Value |
---|---|---|
Candidatus Micrarchaeota | 0.199 | 0.000 |
Candidatus Geothermarchaeota | −0.147 | 0.004 |
Euryarchaeota | 0.118 | 0.020 |
Thermoproteota | −0.046 | 0.369 |
Candidatus Lokiarchaeota | 0.005 | 0.916 |
Nitrososphaerota | −0.004 | 0.933 |
Class | Spearman’s Correlation Coefficient (r) | p-Value |
---|---|---|
Archaeoglobi | 0.1199 | 0.017844 |
Thermoplasmata | 0.088356 | 0.081387 |
Methanococci | −0.08369 | 0.09889 |
Halobacteria | −0.05394 | 0.28799 |
Methanobacteria | 0.042504 | 0.40256 |
Thermoprotei | −0.03954 | 0.43616 |
Nitrososphaeria | 0.03813 | 0.45273 |
Methanomicrobia | −0.02483 | 0.62497 |
Thermococci | −0.02424 | 0.63325 |
Coefficients a | ||||||
---|---|---|---|---|---|---|
Model | Unstandardized Coefficients | Standardized Coefficients | Sig. | 95.0% Confidence Interval for B | ||
B | Std. Error | Beta | Lower Bound | Upper Bound | ||
Thermococcus chitonophagus | 0.186 | 0.405 | 0.032 | 0.647 | −0.610 | 0.981 |
Ferroglobus placidus | 1.868 | 0.288 | 0.278 | 0.000 | 1.301 | 2.435 |
Natrinema sp. J7-2 | 0.872 | 0.081 | 0.461 | 0.000 | 0.713 | 1.031 |
Methanocella conradii | 0.076 | 0.109 | 0.030 | 0.487 | −0.138 | 0.289 |
Sulfolobus sp. E5-1-F | −0.199 | 0.298 | −0.355 | 0.505 | −0.784 | 0.387 |
Pyrolobus fumarii | 0.269 | 0.522 | 0.273 | 0.607 | −0.758 | 1.296 |
Methanobrevibacter olleyae | 0.073 | 0.095 | 0.081 | 0.444 | −0.114 | 0.259 |
Proteins | Spearman’s Correlation Coefficient (r) | p-Value | % of UniprotKB Search Results with Taxonomy: Archaea and Query: Protein | GO Annotation |
---|---|---|---|---|
Beta-galactosidase BgaH | −0.155543828 | 0.002 | 100% | Galactose Metabolic Process |
Methanogen homoaconitase large subunit | −0.11878052 | 0.019 | 100% | Leucine biosynthetic process |
Polyferredoxin protein MvhB | 0.101673216 | 0.045 | 96% | Energy Metabolism |
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Mathlouthi, N.E.H.; Belguith, I.; Yengui, M.; Oumarou Hama, H.; Lagier, J.-C.; Ammar Keskes, L.; Grine, G.; Gdoura, R. The Archaeome’s Role in Colorectal Cancer: Unveiling the DPANN Group and Investigating Archaeal Functional Signatures. Microorganisms 2023, 11, 2742. https://doi.org/10.3390/microorganisms11112742
Mathlouthi NEH, Belguith I, Yengui M, Oumarou Hama H, Lagier J-C, Ammar Keskes L, Grine G, Gdoura R. The Archaeome’s Role in Colorectal Cancer: Unveiling the DPANN Group and Investigating Archaeal Functional Signatures. Microorganisms. 2023; 11(11):2742. https://doi.org/10.3390/microorganisms11112742
Chicago/Turabian StyleMathlouthi, Nour El Houda, Imen Belguith, Mariem Yengui, Hamadou Oumarou Hama, Jean-Christophe Lagier, Leila Ammar Keskes, Ghiles Grine, and Radhouane Gdoura. 2023. "The Archaeome’s Role in Colorectal Cancer: Unveiling the DPANN Group and Investigating Archaeal Functional Signatures" Microorganisms 11, no. 11: 2742. https://doi.org/10.3390/microorganisms11112742