Proteotyping of Campylobacter jejuni by MALDI-TOF MS and Strain Solution Version 2 Software

Ojima-Kato, Teruyo; Nagai, Satomi; Fujita, Akane; Sakata, Junko; Tamura, Hiroto

doi:10.3390/microorganisms11010202

Open AccessArticle

Proteotyping of Campylobacter jejuni by MALDI-TOF MS and Strain Solution Version 2 Software

by

Teruyo Ojima-Kato

^1,2,*

,

Satomi Nagai

¹,

Akane Fujita

¹,

Junko Sakata

³ and

Hiroto Tamura

^1,*

¹

School of Agriculture, Meijo University, Shiogamaguchi, Tenpaku-ku, Nagoya 468-0073, Japan

²

Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan

³

Osaka Institute of Public Health, Nakamichi, Higashinari-ku, Osaka 537-0025, Japan

^*

Authors to whom correspondence should be addressed.

Microorganisms 2023, 11(1), 202; https://doi.org/10.3390/microorganisms11010202

Submission received: 14 December 2022 / Revised: 31 December 2022 / Accepted: 5 January 2023 / Published: 12 January 2023

(This article belongs to the Special Issue New Insights into Epidemiology, Detection and Characterization of Bacterial Pathogens)

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Abstract

Identification of microorganisms by MALDI-TOF MS has become a popular method in the past 20 years. Strain Solution ver. 2 software appended with MALDI-TOF MS enables accurate discrimination of serotypes and strains beyond the genus and species level by creating a theoretical mass-based database. In this study, we constructed a theoretical mass database with the validated biomarkers to proteotype Campylobacter jejuni. Using 10 strains belonging to Campylobacter spp. available from culture collections and 41 Campylobacter jejuni strains isolated from humans and foods, the ribosomal protein subunits L36, L32, S14, L24, L23, L7/L12, and S11 could be selected as the effective biomarkers for the proteotyping of C. jejuni at MALDI-TOF MS. An accurate database of their theoretical mass-based values was constructed by matching these gene DNA sequences and the observed mass peaks. We attempted to automatically classify 41 strains isolated from nature using this database and Strain Solution ver. 2 software, and 38 strains (93%) were correctly classified into the intended group based on the theoretical mass-based values. Thus, the seven biomarkers found in this study and Strain Solution ver. 2 are promising for the proteotyping of C. jejuni by MALDI-TOF MS.

Keywords: Campylobacter jejuni; MALDI-TOF MS; proteotyping; strain solution; theoretical mass database

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MDPI and ACS Style

Ojima-Kato, T.; Nagai, S.; Fujita, A.; Sakata, J.; Tamura, H. Proteotyping of Campylobacter jejuni by MALDI-TOF MS and Strain Solution Version 2 Software. Microorganisms 2023, 11, 202. https://doi.org/10.3390/microorganisms11010202

AMA Style

Ojima-Kato T, Nagai S, Fujita A, Sakata J, Tamura H. Proteotyping of Campylobacter jejuni by MALDI-TOF MS and Strain Solution Version 2 Software. Microorganisms. 2023; 11(1):202. https://doi.org/10.3390/microorganisms11010202

Chicago/Turabian Style

Ojima-Kato, Teruyo, Satomi Nagai, Akane Fujita, Junko Sakata, and Hiroto Tamura. 2023. "Proteotyping of Campylobacter jejuni by MALDI-TOF MS and Strain Solution Version 2 Software" Microorganisms 11, no. 1: 202. https://doi.org/10.3390/microorganisms11010202

APA Style

Ojima-Kato, T., Nagai, S., Fujita, A., Sakata, J., & Tamura, H. (2023). Proteotyping of Campylobacter jejuni by MALDI-TOF MS and Strain Solution Version 2 Software. Microorganisms, 11(1), 202. https://doi.org/10.3390/microorganisms11010202

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Proteotyping of Campylobacter jejuni by MALDI-TOF MS and Strain Solution Version 2 Software

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