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Article

Identification and Molecular Characterization of the Operon Required for L-Asparagine Utilization in Corynebacterium glutamicum

1
Research Institute of Innovative Technology for the Earth (RITE), 9-2 Kizugawadai, Kizugawa 619-0292, Japan
2
Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Hisashi Kawasaki and Yoshihiro Usuda
Microorganisms 2022, 10(5), 1002; https://doi.org/10.3390/microorganisms10051002
Received: 6 April 2022 / Revised: 30 April 2022 / Accepted: 7 May 2022 / Published: 10 May 2022
Understanding the metabolic pathways of amino acids and their regulation is important for the rational metabolic engineering of amino acid production. The catabolic pathways of L-asparagine and L-aspartate are composed of transporters for amino acid uptake and asparaginase and aspartase, which are involved in the sequential deamination to fumarate. However, knowledge of the catabolic genes for asparagine in bacteria of the Actinobacteria class has been limited. In this study, we identified and characterized the ans operon required for L-Asn catabolism in Corynebacterium glutamicum R. The operon consisted of genes encoding a transcriptional regulator (AnsR), asparaginase (AnsA2), aspartase (AspA2), and permease (AnsP). The enzymes and permease encoded in the operon were shown to be essential for L-Asn utilization, but another asparaginase, AnsA1, and aspartase, AspA1, were not essential. Expression analysis revealed that the operon was induced in response to extracellular L-Asn and was transcribed as a leaderless mRNA. The DNA-binding assay demonstrated that AnsR acted as a transcriptional repressor of the operon by binding to the inverted repeat at its 5′-end region. The AnsR binding was inhibited by L-Asn. This study provides insights into the functions and regulatory mechanisms of similar operon-like clusters in related bacteria. View Full-Text
Keywords: asparaginase; aspartase; Corynebacterium glutamicum; L-asparagine; L-aspartate; transcriptional regulation asparaginase; aspartase; Corynebacterium glutamicum; L-asparagine; L-aspartate; transcriptional regulation
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MDPI and ACS Style

Toyoda, K.; Sugaya, R.; Domon, A.; Suda, M.; Hiraga, K.; Inui, M. Identification and Molecular Characterization of the Operon Required for L-Asparagine Utilization in Corynebacterium glutamicum. Microorganisms 2022, 10, 1002. https://doi.org/10.3390/microorganisms10051002

AMA Style

Toyoda K, Sugaya R, Domon A, Suda M, Hiraga K, Inui M. Identification and Molecular Characterization of the Operon Required for L-Asparagine Utilization in Corynebacterium glutamicum. Microorganisms. 2022; 10(5):1002. https://doi.org/10.3390/microorganisms10051002

Chicago/Turabian Style

Toyoda, Koichi, Riki Sugaya, Akihiro Domon, Masako Suda, Kazumi Hiraga, and Masayuki Inui. 2022. "Identification and Molecular Characterization of the Operon Required for L-Asparagine Utilization in Corynebacterium glutamicum" Microorganisms 10, no. 5: 1002. https://doi.org/10.3390/microorganisms10051002

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