Next Article in Journal
Cutaneous Complications of mRNA and AZD1222 COVID-19 Vaccines: A Worldwide Review
Previous Article in Journal
Wintertime Simulations Induce Changes in the Structure, Diversity and Function of Antarctic Sea Ice-Associated Microbial Communities
Article

Cellular Responses Required for Oxidative Stress Tolerance of the Necrotrophic Fungus Alternaria alternata, Causal Agent of Pear Black Spot

1
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China
2
Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Rishon LeZion 50250, Israel
*
Author to whom correspondence should be addressed.
Academic Editor: Hermann J. Heipieper
Microorganisms 2022, 10(3), 621; https://doi.org/10.3390/microorganisms10030621
Received: 11 February 2022 / Revised: 11 March 2022 / Accepted: 12 March 2022 / Published: 15 March 2022
(This article belongs to the Section Plant Microbe Interactions)
To establish successful infections in host plants, pathogenic fungi must sense and respond to an array of stresses, such as oxidative stress. In this study, we systematically analyzed the effects of 30 mM H2O2 treatment on reactive oxygen species (ROS) metabolism in Alternaria alternata. Results showed that 30 mM H2O2 treatment lead to increased O2− generation rate and H2O2 content, and simultaneously, increased the activities and transcript levels of NADPH oxidase (NOX). The activities and gene expression levels of enzymes related with ascorbic acid-glutathione cycle (AsA-GSH cycle) and thioredoxin systems, including superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), ascorbate peroxidase (AXP) and thioredoxin (TrxR), were remarkably enhanced by 30 mM H2O2 stress treatment. Additionally, 30 mM H2O2 treatment decreased the glutathione (GSH) content, whereas it increased the amount of oxidized glutathione (GSSG), dehydroascorbate (DHA) and ascorbic acid (AsA). These results revealed that cellular responses are required for oxidative stress tolerance of the necrotrophic fungus A. alternata. View Full-Text
Keywords: Alternaria alternata; oxidative stress; AsA-GSH cycle; thioredoxin system; redox balance Alternaria alternata; oxidative stress; AsA-GSH cycle; thioredoxin system; redox balance
Show Figures

Figure 1

MDPI and ACS Style

Zhang, M.; Zhang, Y.; Li, Y.; Bi, Y.; Mao, R.; Yang, Y.; Jiang, Q.; Prusky, D. Cellular Responses Required for Oxidative Stress Tolerance of the Necrotrophic Fungus Alternaria alternata, Causal Agent of Pear Black Spot. Microorganisms 2022, 10, 621. https://doi.org/10.3390/microorganisms10030621

AMA Style

Zhang M, Zhang Y, Li Y, Bi Y, Mao R, Yang Y, Jiang Q, Prusky D. Cellular Responses Required for Oxidative Stress Tolerance of the Necrotrophic Fungus Alternaria alternata, Causal Agent of Pear Black Spot. Microorganisms. 2022; 10(3):621. https://doi.org/10.3390/microorganisms10030621

Chicago/Turabian Style

Zhang, Miao, Yandong Zhang, Yongcai Li, Yang Bi, Renyan Mao, Yangyang Yang, Qianqian Jiang, and Dov Prusky. 2022. "Cellular Responses Required for Oxidative Stress Tolerance of the Necrotrophic Fungus Alternaria alternata, Causal Agent of Pear Black Spot" Microorganisms 10, no. 3: 621. https://doi.org/10.3390/microorganisms10030621

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop