Cellular Responses Required for Oxidative Stress Tolerance of the Necrotrophic Fungus Alternaria alternata, Causal Agent of Pear Black Spot

Zhang, Miao; Zhang, Yandong; Li, Yongcai; Bi, Yang; Mao, Renyan; Yang, Yangyang; Jiang, Qianqian; Prusky, Dov

doi:10.3390/microorganisms10030621

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Cellular Responses Required for Oxidative Stress Tolerance of the Necrotrophic Fungus Alternaria alternata, Causal Agent of Pear Black Spot

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College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China

Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Rishon LeZion 50250, Israel

Author to whom correspondence should be addressed.

Academic Editor: Hermann J. Heipieper

Microorganisms 2022, 10(3), 621; https://doi.org/10.3390/microorganisms10030621

Received: 11 February 2022 / Revised: 11 March 2022 / Accepted: 12 March 2022 / Published: 15 March 2022

(This article belongs to the Section Plant Microbe Interactions)

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Abstract

To establish successful infections in host plants, pathogenic fungi must sense and respond to an array of stresses, such as oxidative stress. In this study, we systematically analyzed the effects of 30 mM H₂O₂ treatment on reactive oxygen species (ROS) metabolism in Alternaria alternata. Results showed that 30 mM H₂O₂ treatment lead to increased O²⁻ generation rate and H₂O₂ content, and simultaneously, increased the activities and transcript levels of NADPH oxidase (NOX). The activities and gene expression levels of enzymes related with ascorbic acid-glutathione cycle (AsA-GSH cycle) and thioredoxin systems, including superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), ascorbate peroxidase (AXP) and thioredoxin (TrxR), were remarkably enhanced by 30 mM H₂O₂ stress treatment. Additionally, 30 mM H₂O₂ treatment decreased the glutathione (GSH) content, whereas it increased the amount of oxidized glutathione (GSSG), dehydroascorbate (DHA) and ascorbic acid (AsA). These results revealed that cellular responses are required for oxidative stress tolerance of the necrotrophic fungus A. alternata. View Full-Text

Keywords: Alternaria alternata; oxidative stress; AsA-GSH cycle; thioredoxin system; redox balance

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MDPI and ACS Style

Zhang, M.; Zhang, Y.; Li, Y.; Bi, Y.; Mao, R.; Yang, Y.; Jiang, Q.; Prusky, D. Cellular Responses Required for Oxidative Stress Tolerance of the Necrotrophic Fungus Alternaria alternata, Causal Agent of Pear Black Spot. Microorganisms 2022, 10, 621. https://doi.org/10.3390/microorganisms10030621

AMA Style

Zhang M, Zhang Y, Li Y, Bi Y, Mao R, Yang Y, Jiang Q, Prusky D. Cellular Responses Required for Oxidative Stress Tolerance of the Necrotrophic Fungus Alternaria alternata, Causal Agent of Pear Black Spot. Microorganisms. 2022; 10(3):621. https://doi.org/10.3390/microorganisms10030621

Chicago/Turabian Style

Zhang, Miao, Yandong Zhang, Yongcai Li, Yang Bi, Renyan Mao, Yangyang Yang, Qianqian Jiang, and Dov Prusky. 2022. "Cellular Responses Required for Oxidative Stress Tolerance of the Necrotrophic Fungus Alternaria alternata, Causal Agent of Pear Black Spot" Microorganisms 10, no. 3: 621. https://doi.org/10.3390/microorganisms10030621

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Cellular Responses Required for Oxidative Stress Tolerance of the Necrotrophic Fungus Alternaria alternata, Causal Agent of Pear Black Spot

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