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Article

Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses

1
Grupo Virología Humana, Instituto de Biología Molecular y Celular de Rosario (CONICET), Suipacha 590, Rosario 2000, Argentina
2
Área Virología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario 2000, Argentina
3
DETx MOL S.A., Centro Científico Tecnológico CONICET Rosario, Ocampo y Esmeralda, Rosario 2000, Argentina
4
Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, SI-1000 Ljubljana, Slovenia
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Museo Provincial de Ciencias Naturales “Dr. Ángel Gallardo”, San Lorenzo 1949, Rosario 2000, Argentina
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Programa de Conservación de los Murciélagos de Argentina, Miguel Lillo 251, San Miguel de Tucumán 4000, Argentina
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Instituto PIDBA (Programa de Investigaciones de Biodiversidad Argentina), Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán, Miguel Lillo 205, San Miguel de Tucumán 4000, Argentina
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Área Estadística y Procesamiento de Datos, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario 2000, Argentina
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Alex D. Greenwood
Microorganisms 2022, 10(2), 266; https://doi.org/10.3390/microorganisms10020266
Received: 29 December 2021 / Revised: 17 January 2022 / Accepted: 18 January 2022 / Published: 24 January 2022
(This article belongs to the Special Issue Viruses of Wild Mammals)
Bats are natural reservoirs of a variety of zoonotic viruses, many of which cause severe human diseases. Characterizing viruses of bats inhabiting different geographical regions is important for understanding their viral diversity and for detecting viral spillovers between animal species. Herein, the diversity of DNA viruses of five arthropodophagous bat species from Argentina was investigated using metagenomics. Fecal samples of 29 individuals from five species (Tadarida brasiliensis, Molossus molossus, Eumops bonariensis, Eumops patagonicus, and Eptesicus diminutus) living at two different geographical locations, were investigated. Enriched viral DNA was sequenced using Illumina MiSeq, and the reads were trimmed and filtered using several bioinformatic approaches. The resulting nucleotide sequences were subjected to viral taxonomic classification. In total, 4,520,370 read pairs were sequestered by sequencing, and 21.1% of them mapped to viral taxa. Circoviridae and Genomoviridae were the most prevalent among vertebrate viral families in all bat species included in this study. Samples from the T. brasiliensis colony exhibited lower viral diversity than samples from other species of New World bats. We characterized 35 complete genome sequences of novel viruses. These findings provide new insights into the global diversity of bat viruses in poorly studied species, contributing to prevention of emerging zoonotic diseases and to conservation policies for endangered species. View Full-Text
Keywords: Chiroptera; metagenomics; virome; virus identification; Cressdnaviricota; Cossaviricota; Anelloviridae Chiroptera; metagenomics; virome; virus identification; Cressdnaviricota; Cossaviricota; Anelloviridae
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MDPI and ACS Style

Bolatti, E.M.; Viarengo, G.; Zorec, T.M.; Cerri, A.; Montani, M.E.; Hosnjak, L.; Casal, P.E.; Bortolotto, E.; Di Domenica, V.; Chouhy, D.; Allasia, M.B.; Barquez, R.M.; Poljak, M.; Giri, A.A. Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses. Microorganisms 2022, 10, 266. https://doi.org/10.3390/microorganisms10020266

AMA Style

Bolatti EM, Viarengo G, Zorec TM, Cerri A, Montani ME, Hosnjak L, Casal PE, Bortolotto E, Di Domenica V, Chouhy D, Allasia MB, Barquez RM, Poljak M, Giri AA. Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses. Microorganisms. 2022; 10(2):266. https://doi.org/10.3390/microorganisms10020266

Chicago/Turabian Style

Bolatti, Elisa M., Gastón Viarengo, Tomaz M. Zorec, Agustina Cerri, María E. Montani, Lea Hosnjak, Pablo E. Casal, Eugenia Bortolotto, Violeta Di Domenica, Diego Chouhy, María Belén Allasia, Rubén M. Barquez, Mario Poljak, and Adriana A. Giri. 2022. "Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses" Microorganisms 10, no. 2: 266. https://doi.org/10.3390/microorganisms10020266

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