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Volume 10
Issue 11
10.3390/microorganisms10112263
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Article

Roseburia intestinalis Modulates PYY Expression in a New a Multicellular Model including Enteroendocrine Cells

by
Thomas Gautier
1,
Nelly Fahet
1,
Zohreh Tamanai-Shacoori
1,
Nolwenn Oliviero
1,
Marielle Blot
2,
Aurélie Sauvager
2,
Agnes Burel
3,
Sandrine David-Le Gall
1,
Sophie Tomasi
2,
Sophie Blat
1 and
Latifa Bousarghin
1,*
1
Institut NUMECAN, INSERM, Univ Rennes, INRAE, F-35000 Rennes, France
2
ISCR (Institut des Sciences Chimiques de Rennes)-UMR CNRS 6226, Univ Rennes, CNRS, F-35000 Rennes, France
3
Plateforme Microscopie Electronique MRic/ISFR Biosit/Campus Santé, Univ Rennes, F-35000 Rennes, France
*
Author to whom correspondence should be addressed.
Microorganisms 2022, 10(11), 2263; https://doi.org/10.3390/microorganisms10112263
Submission received: 14 October 2022 / Revised: 8 November 2022 / Accepted: 10 November 2022 / Published: 15 November 2022
(This article belongs to the Special Issue Effects of Probiotics on Health)
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Abstract

The gut microbiota contributes to human health and disease; however, the mechanisms by which commensal bacteria interact with the host are still unclear. To date, a number of in vitro systems have been designed to investigate the host–microbe interactions. In most of the intestinal models, the enteroendocrine cells, considered as a potential link between gut bacteria and several human diseases, were missing. In the present study, we have generated a new model by adding enteroendocrine cells (ECC) of L-type (NCI-H716) to the one that we have previously described including enterocytes, mucus, and M cells. After 21 days of culture with the other cells, enteroendocrine-differentiated NCI-H716 cells showed neuropods at their basolateral side and expressed their specific genes encoding proglucagon (GCG) and chromogranin A (CHGA). We showed that this model could be stimulated by commensal bacteria playing a key role in health, Roseburia intestinalis and Bacteroides fragilis, but also by a pathogenic strain such as Salmonella Heidelberg. Moreover, using cell-free supernatants of B. fragilis and R. intestinalis, we have shown that R. intestinalis supernatant induced a significant increase in IL-8 and PYY but not in GCG gene expression, while B. fragilis had no impact. Our data indicated that R. intestinalis produced short chain fatty acids (SCFAs) such as butyrate whereas B. fragilis produced more propionate. However, these SCFAs were probably not the only metabolites implicated in PYY expression since butyrate alone had no effect. In conclusion, our new quadricellular model of gut epithelium could be an effective tool to highlight potential beneficial effects of bacteria or their metabolites, in order to develop new classes of probiotics.
Keywords: hormone-producing cells; quadricellular model; gut commensal bacteria; butyrate; proglucagon; peptide tyrosine tyrosine hormone-producing cells; quadricellular model; gut commensal bacteria; butyrate; proglucagon; peptide tyrosine tyrosine

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MDPI and ACS Style

Gautier, T.; Fahet, N.; Tamanai-Shacoori, Z.; Oliviero, N.; Blot, M.; Sauvager, A.; Burel, A.; Gall, S.D.-L.; Tomasi, S.; Blat, S.; et al. Roseburia intestinalis Modulates PYY Expression in a New a Multicellular Model including Enteroendocrine Cells. Microorganisms 2022, 10, 2263. https://doi.org/10.3390/microorganisms10112263

AMA Style

Gautier T, Fahet N, Tamanai-Shacoori Z, Oliviero N, Blot M, Sauvager A, Burel A, Gall SD-L, Tomasi S, Blat S, et al. Roseburia intestinalis Modulates PYY Expression in a New a Multicellular Model including Enteroendocrine Cells. Microorganisms. 2022; 10(11):2263. https://doi.org/10.3390/microorganisms10112263

Chicago/Turabian Style

Gautier, Thomas, Nelly Fahet, Zohreh Tamanai-Shacoori, Nolwenn Oliviero, Marielle Blot, Aurélie Sauvager, Agnes Burel, Sandrine David-Le Gall, Sophie Tomasi, Sophie Blat, and et al. 2022. "Roseburia intestinalis Modulates PYY Expression in a New a Multicellular Model including Enteroendocrine Cells" Microorganisms 10, no. 11: 2263. https://doi.org/10.3390/microorganisms10112263

APA Style

Gautier, T., Fahet, N., Tamanai-Shacoori, Z., Oliviero, N., Blot, M., Sauvager, A., Burel, A., Gall, S. D.-L., Tomasi, S., Blat, S., & Bousarghin, L. (2022). Roseburia intestinalis Modulates PYY Expression in a New a Multicellular Model including Enteroendocrine Cells. Microorganisms, 10(11), 2263. https://doi.org/10.3390/microorganisms10112263

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