Accelerated Bioconversion of Chemically Solubilized Lignite Solution to Methane by Methanogenic Consortium: Experimental Results and Their Application to the Subsurface Cultivation and Gasification Method
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Preparation of Chemically Solubilized Lignite Solution
2.2. Construction of Methanogenic Enrichment Cultures
2.3. Microcosm Experiments
2.4. Analytical Procedures
2.5. DNA Extraction and Next-Generation Sequencing
2.6. Real-Time Quantitative PCR
2.7. Statistical Analysis
2.8. Sequence Accession Numbers
3. Results
3.1. Methane Production in Microcosms Supplemented with Chemically Solubilized Lignite Solution Using the Microbial Consortium, mMEC
3.1.1. Characteristics of the MECs
3.1.2. Methane Production Using the mMEC
3.1.3. Microbial Composition in Microcosms
3.2. Methane Production in Microcosms Supplemented with Chemically Solubilized Lignite Solution Using the Microbial Consortium, SAL25-2
3.2.1. Methane Production Using the SAL25-2
3.2.2. Microbial Composition in Microcosms
3.3. Real-Time Quantitative PCR Analysis of Microcosms
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | Original Solution | Concentrations in Microcosms | ||
---|---|---|---|---|
pH | 2.4 | |||
Dissolved total organic carbon [mg/L TOC] | 2800 | 1000 | 100 | 10 |
Dissolved nitrogen [mg/L] | 145 | 52.0 | 5.2 | 0.5 |
Acetate [mg/L] | 937 | 335 | 33.5 | 3.3 |
Formate [mg/L] | 656 | 235 | 23.5 | 2.3 |
Cl− [mg/L] | 5.40 | 1.9 | 0.2 | 0.02 |
NO3− [mg/L] | 7.50 | 2.7 | 0.3 | 0.03 |
SO42− [mg/L] | 102 | 36.4 | 3.6 | 0.4 |
Malonate [mg/L] | 1060 | 378 | 37.8 | 3.8 |
Succinate [mg/L] | 241 | 86.0 | 8.6 | 0.9 |
Oxalate [mg/L] | 1010 | |||
H2O2 [%] | <10−4 |
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Ueno, A.; Tamazawa, S.; Tamamura, S.; Murakami, T.; Kiyama, T.; Inomata, H.; Aramaki, N.; Yoshida, K.; Yamaguchi, S.; Aoyama, H.; et al. Accelerated Bioconversion of Chemically Solubilized Lignite Solution to Methane by Methanogenic Consortium: Experimental Results and Their Application to the Subsurface Cultivation and Gasification Method. Microorganisms 2022, 10, 1984. https://doi.org/10.3390/microorganisms10101984
Ueno A, Tamazawa S, Tamamura S, Murakami T, Kiyama T, Inomata H, Aramaki N, Yoshida K, Yamaguchi S, Aoyama H, et al. Accelerated Bioconversion of Chemically Solubilized Lignite Solution to Methane by Methanogenic Consortium: Experimental Results and Their Application to the Subsurface Cultivation and Gasification Method. Microorganisms. 2022; 10(10):1984. https://doi.org/10.3390/microorganisms10101984
Chicago/Turabian StyleUeno, Akio, Satoshi Tamazawa, Shuji Tamamura, Takuma Murakami, Tamotsu Kiyama, Hidenori Inomata, Noritaka Aramaki, Kunihiko Yoshida, Shinji Yamaguchi, Hideo Aoyama, and et al. 2022. "Accelerated Bioconversion of Chemically Solubilized Lignite Solution to Methane by Methanogenic Consortium: Experimental Results and Their Application to the Subsurface Cultivation and Gasification Method" Microorganisms 10, no. 10: 1984. https://doi.org/10.3390/microorganisms10101984