MCMV Dissemination from Latently-Infected Allografts Following Transplantation into Pre-Tolerized Recipients
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. CMV Infection and Establishment of Latency
2.3. Islet Transplantation
2.4. Donor Splenocyte Transfusion
2.5. Kidney Transplantation
2.6. Cell Isolation and Flow Analyses
2.7. Histology
2.8. Viral DNA Analyses
2.9. Statistical Analysis
3. Results
3.1. Experimental Approach and Induction of Operational Tolerance
3.2. Are ECDIsp-Treated Islet Recipients Tolerant to Secondary Solid Organ Alloantigen Challenge with or without Latently Infected CMV?
3.3. Ecdisp-Treated Recipients Transplanted with Latently-Infected Allografts Exhibit Increased Trends of Innate Inflammation and Markers Of Macrophage (Mɸ) Activation
3.4. Ecdisp-Treated Recipients Transplanted with Latently-Infected Allografts Exhibit Reduced PD1+ CD8 T Effector Cells
3.5. Pre-Tolerized Recipients Were Permissive to Viral Dissemination
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Shah, S.; DeBerge, M.; Iovane, A.; Yan, S.; Qiu, L.; Wang, J.-J.; Kanwar, Y.S.; Hummel, M.; Zhang, Z.J.; Abecassis, M.M.; et al. MCMV Dissemination from Latently-Infected Allografts Following Transplantation into Pre-Tolerized Recipients. Pathogens 2020, 9, 607. https://doi.org/10.3390/pathogens9080607
Shah S, DeBerge M, Iovane A, Yan S, Qiu L, Wang J-J, Kanwar YS, Hummel M, Zhang ZJ, Abecassis MM, et al. MCMV Dissemination from Latently-Infected Allografts Following Transplantation into Pre-Tolerized Recipients. Pathogens. 2020; 9(8):607. https://doi.org/10.3390/pathogens9080607
Chicago/Turabian StyleShah, Sahil, Matthew DeBerge, Andre Iovane, Shixian Yan, Longhui Qiu, Jiao-Jing Wang, Yashpal S. Kanwar, Mary Hummel, Zheng J. Zhang, Michael M. Abecassis, and et al. 2020. "MCMV Dissemination from Latently-Infected Allografts Following Transplantation into Pre-Tolerized Recipients" Pathogens 9, no. 8: 607. https://doi.org/10.3390/pathogens9080607