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Open AccessArticle

Evaluation of Interferon-Gamma Polymorphisms as a Risk Factor in Feline Infectious Peritonitis Development in Non-Pedigree Cats—A Large Cohort Study

1
Langford Vets, University of Bristol, Langford BS40 5DU, UK
2
Bristol Veterinary School, University of Bristol, Langford BS40 5DU, UK
3
Department of Veterinary Pathology and Public Health, Institute of Veterinary Science, University of Liverpool, Neston CH64 7TE, UK
4
The Linnaeus Group, Shirley, Solihull B90 1BN, UK
5
Circa Healthcare, 116 Dundas Street, Edinburgh EH3 5DQ, UK
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Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland
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Institute of Global Health, University of Liverpool, Liverpool L1 8JX, UK
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(7), 535; https://doi.org/10.3390/pathogens9070535
Received: 27 May 2020 / Revised: 18 June 2020 / Accepted: 29 June 2020 / Published: 3 July 2020
(This article belongs to the Special Issue Feline Infectious Peritonitis)
Feline infectious peritonitis (FIP) is a common infectious cause of death in cats, with heritable host factors associated with altered risk of disease. To assess the role of feline interferon-gamma gene (fIFNG) variants in this risk, the allele frequencies of two single nucleotide polymorphisms (SNPs) (g.401 and g.408) were determined for non-pedigree cats either with confirmed FIP (n = 59) or from the general population (cats enrolled in a large lifetime longitudinal study; n = 264). DNA was extracted from buccal swabs or tissue samples. A pyrosequencing assay to characterize the fIFNG SNPs was designed, optimized and subsequently performed on all samples. Genotype and allele frequency were calculated for each population. Characterization of the target SNPs was possible for 56 of the cats with FIP and 263 of the cats from the general population. The SNPs were in complete linkage disequilibrium with each other. There was an association between FIP status and genotype (χ2; p = 0.028), with a reduced risk of developing FIP (χ2; p = 0.0077) associated with the genotype TT at both positions. These results indicate that, although fIFNG variants may be associated with altered risk of disease, the prevalence of individual variants within both populations limits application of their characterization to breeding purposes. View Full-Text
Keywords: cohort study; feline coronavirus; gamma interferon; genetic risk factor; pyrosequencing cohort study; feline coronavirus; gamma interferon; genetic risk factor; pyrosequencing
MDPI and ACS Style

Barker, E.N.; Lait, P.; Ressel, L.; Blackwell, E.-J.; Tasker, S.; Kedward-Dixon, H.; Kipar, A.; Helps, C.R. Evaluation of Interferon-Gamma Polymorphisms as a Risk Factor in Feline Infectious Peritonitis Development in Non-Pedigree Cats—A Large Cohort Study. Pathogens 2020, 9, 535. https://doi.org/10.3390/pathogens9070535

AMA Style

Barker EN, Lait P, Ressel L, Blackwell E-J, Tasker S, Kedward-Dixon H, Kipar A, Helps CR. Evaluation of Interferon-Gamma Polymorphisms as a Risk Factor in Feline Infectious Peritonitis Development in Non-Pedigree Cats—A Large Cohort Study. Pathogens. 2020; 9(7):535. https://doi.org/10.3390/pathogens9070535

Chicago/Turabian Style

Barker, Emi N.; Lait, Philippa; Ressel, Lorenzo; Blackwell, Emily-Jayne; Tasker, Séverine; Kedward-Dixon, Helen; Kipar, Anja; Helps, Christopher R. 2020. "Evaluation of Interferon-Gamma Polymorphisms as a Risk Factor in Feline Infectious Peritonitis Development in Non-Pedigree Cats—A Large Cohort Study" Pathogens 9, no. 7: 535. https://doi.org/10.3390/pathogens9070535

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