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Open AccessArticle

ZnO Nanoflower-Based NanoPCR as an Efficient Diagnostic Tool for Quick Diagnosis of Canine Vector-Borne Pathogens

1
Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan 570228, China
2
State Key Laboratory of Marine Resource Utilization in South China Sea, College of Material Science and Engineering, Haikou, Hainan 570228, China
3
State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Provincial Key Laboratory of Research on Utilization of Si-Zr-Ti Resources, College of Material Science and Engineering, Hainan University, Haikou, Hainan 570228, China
4
State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, Hainan 570228, China
*
Authors to whom correspondence should be addressed.
Pathogens 2020, 9(2), 122; https://doi.org/10.3390/pathogens9020122
Received: 1 January 2020 / Revised: 27 January 2020 / Accepted: 12 February 2020 / Published: 14 February 2020
(This article belongs to the Special Issue Canine and Feline Infectious Diseases)
: Polymerase chain reaction (PCR) is a unique technique in molecular biology and biotechnology for amplifying target DNA strands, and is also considered as a gold standard for the diagnosis of many canine diseases as well as many other infectious diseases. However, PCR still faces many challenges and issues related to its sensitivity, specificity, efficiency, and turnaround time. To address these issues, we described the use of unique ZnO nanoflowers in PCR reaction and an efficient ZnO nanoflower-based PCR (nanoPCR) for the molecular diagnosis of canine vector-borne diseases (CVBDs). A total of 1 mM of an aqueous solution of ZnO nanoflowers incorporated in PCR showed a significant enhancement of the PCR assay with respect to its sensitivity and specificity for the diagnosis of two important CVBDs, Babesia canis vogeli and Hepatozoon canis. Interestingly, it drastically reduced the turnaround time of the PCR assay without compromising the yield of the amplified DNA, which can be of benefit for veterinary practitioners for the improved management of diseases. This can be attributed to the favorable adsorption of ZnO nanoflowers to the DNA and thermal conductivity of ZnO nanoflowers. The unique ZnO nanoflower-assisted nanoPCR greatly improved the yield, purity, and quality of the amplified products, but the mechanism behind these properties and the effects and changes due to the different concentrations of ZnO nanoflowers in the PCR system needs to be further studied.
Keywords: ZnO nanoflowers; PCR; nanomaterial-assisted polymerase chain reaction; nanoPCR; canine vector-borne diseases; Babesia canis vogeli; Hepatozoon canis ZnO nanoflowers; PCR; nanomaterial-assisted polymerase chain reaction; nanoPCR; canine vector-borne diseases; Babesia canis vogeli; Hepatozoon canis
MDPI and ACS Style

Upadhyay, A.; Yang, H.; Zaman, B.; Zhang, L.; Wu, Y.; Wang, J.; Zhao, J.; Liao, C.; Han, Q. ZnO Nanoflower-Based NanoPCR as an Efficient Diagnostic Tool for Quick Diagnosis of Canine Vector-Borne Pathogens. Pathogens 2020, 9, 122.

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