Next Article in Journal
Effect of Litter Treatment on Campylobacter jejuni in Broilers and on Cecal Microbiota
Next Article in Special Issue
Identification of Serogroups Australis and Icterohaemorrhagiae in Two Dogs with a Severe Form of Acute Leptospirosis in Italy
Previous Article in Journal
The Human Coronavirus Disease COVID-19: Its Origin, Characteristics, and Insights into Potential Drugs and Its Mechanisms
Open AccessArticle

Serological Survey and Molecular Typing Reveal New Leptospira Serogroup Pomona Strains among Pigs of Northern Italy

1
National Reference Centre for Animal Leptospirosis (NRCL), Istituto Zooprofilattico Sperimentale della Lombardia e dell’ Emilia Romagna “Bruno Ubertini”, via Bianchi 7/9, 25121 Brescia, Italy
2
Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell’ Emilia Romagna “Bruno Ubertini”, Strada dei Mercati 13/a, 43126 Parma, Italy
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(5), 332; https://doi.org/10.3390/pathogens9050332
Received: 2 April 2020 / Revised: 25 April 2020 / Accepted: 27 April 2020 / Published: 29 April 2020
(This article belongs to the Special Issue Leptospira infections in Domestic and Wild Animal)
Swine act as both maintenance and incidental hosts of pathogenic Leptospira spp. Here, a serological test was performed on 131,660 pig sera collected between 2002 and 2017 from 4715 farms in Northern Italy. A positivity rate of 13.05% was determined. Australis was the most frequently identified serogroup (77.29%), followed by Pomona (18.47%), Tarassovi (1.51%) and Icterohaemorrhagie (1.40%). Culture isolation and real-time Polymerase chain reaction (PCR) were carried out on 347 kidneys and 470 clinical samples, respectively. Overall, 133 strains were cultured successfully and 43 randomly chosen isolates were identified as serogroup Pomona. Multi-locus sequence typing (MLST) revealed that 41 isolates and 8 DNA extracted from biological samples belonged to sequence type 140. Using a multiple-locus, variable-number tandem repeat analysis, 43 samples produced identical profiles but, after 2014, three new Leptospira interrogans serogroup Pomona genotypes were observed. Interestingly, two isolates showed new MLST profiles and an unclassified identification by monoclonal antibodies. The 16S rRNA gene sequencing clustered them into L. kirschneri species and a core genome MLST analysis revealed an allelic identity of 96% compared with Mozdok strains. Genotyping allowed us to discriminate leptospires and to identify new emerging strains. The accurate identification of infective strains is required for formulating preventive methods and intervention strategies. View Full-Text
Keywords: Leptospirosis; pig; MAT; real-time PCR; genotyping; epidemiology Leptospirosis; pig; MAT; real-time PCR; genotyping; epidemiology
Show Figures

Figure 1

MDPI and ACS Style

Bertasio, C.; Papetti, A.; Scaltriti, E.; Tagliabue, S.; D’Incau, M.; Boniotti, M.B. Serological Survey and Molecular Typing Reveal New Leptospira Serogroup Pomona Strains among Pigs of Northern Italy. Pathogens 2020, 9, 332.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop