Next Article in Journal
Comparative Characterization of G Protein α Subunits in Aspergillus fumigatus
Next Article in Special Issue
Transmission of Antibiotic Resistant Bacteria and Genes: Unveiling the Jigsaw Pieces of a One Health Problem
Previous Article in Journal
Analysis of the Role of TpUB05 Antigen from Theileria parva in Immune Responses to Malaria in Humans Compared to Its Homologue in Plasmodium falciparum the UB05 Antigen
Previous Article in Special Issue
Detection of β-Lactamase-Producing Enterococcus faecalis and Vancomycin-Resistant Enterococcus faecium Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
Letter

Acquired AmpC β-Lactamases among Enterobacteriaceae from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal

1
UCIBIO-REQUIMTE, Laboratory of Microbiology, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal
2
FP-ENAS/CEBIMED, Faculty of Health Sciences, University Fernando Pessoa, 4200-250 Porto, Portugal
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(4), 273; https://doi.org/10.3390/pathogens9040273
Received: 10 March 2020 / Revised: 7 April 2020 / Accepted: 7 April 2020 / Published: 9 April 2020
We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing Enterobacteriaceae from different non-clinical environments in Portugal. We analysed 880 Enterobacteriaceae resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-CeuI-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among Enterobacteriaceae from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing Escherichia coli from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013–2014 versus 0% in 2001–2004], which is in accordance with the trend observed in other European countries. CMY-2-producing E. coli belonged to B22-ST4953 (n = 2, HH), A0-ST665 (n = 1, HH) or A1-ST48 (n = 1, UCC) clones. blaCMY-2 was identified in non-typeable and IncA/C2 plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing Enterobacteriaceae occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of blaqAmpC in non-clinical settings. View Full-Text
Keywords: CMY-2; Escherichia coli; ST48; ST665; plasmids CMY-2; Escherichia coli; ST48; ST665; plasmids
Show Figures

Figure 1

MDPI and ACS Style

Gonçalves Ribeiro, T.; Novais, Â.; Machado, E.; Peixe, L. Acquired AmpC β-Lactamases among Enterobacteriaceae from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal. Pathogens 2020, 9, 273. https://doi.org/10.3390/pathogens9040273

AMA Style

Gonçalves Ribeiro T, Novais Â, Machado E, Peixe L. Acquired AmpC β-Lactamases among Enterobacteriaceae from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal. Pathogens. 2020; 9(4):273. https://doi.org/10.3390/pathogens9040273

Chicago/Turabian Style

Gonçalves Ribeiro, Teresa, Ângela Novais, Elisabete Machado, and Luísa Peixe. 2020. "Acquired AmpC β-Lactamases among Enterobacteriaceae from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal" Pathogens 9, no. 4: 273. https://doi.org/10.3390/pathogens9040273

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop