Next Article in Journal
A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria
Next Article in Special Issue
A Global Analysis of Enzyme Compartmentalization to Glycosomes
Previous Article in Journal
A qPCR-Based Survey of Haplosporidium nelsoni and Perkinsus spp. in the Eastern Oyster, Crassostrea virginica in Maine, USA
Previous Article in Special Issue
Common Structural Patterns in the Maxicircle Divergent Region of Trypanosomatidae
Open AccessArticle

Inventory and Evolution of Mitochondrion-localized Family A DNA Polymerases in Euglenozoa

1
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan
2
Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan
3
Japan Agency for Marine-Earth Science and Technology, Yokosuka 236-0001, Japan
4
Department of Applied Chemistry and Food Science, Fukui University of Technology, Fukui 910-8505, Japan
5
Graduate School of Engineering, Fukui University of Technology, Fukui 910-8505, Japan
6
Department of Gene Function and Phenomics, National Institute of Genetics, Mishima 411-8540, Japan
7
Department of Parasitology, Charles University, Faculty of Science, BIOCEV, 252 42 Vestec, Czech Republic
8
Department of Zoology, National Museum of Nature and Science, Tsukuba 305-0005, Japan
9
Center for Computational Sciences, University of Tsukuba, Tsukuba 305-8577, Japan
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(4), 257; https://doi.org/10.3390/pathogens9040257
Received: 1 February 2020 / Revised: 29 March 2020 / Accepted: 30 March 2020 / Published: 1 April 2020
(This article belongs to the Special Issue Kinetoplastid Phylogenomics and Evolution)
The order Trypanosomatida has been well studied due to its pathogenicity and the unique biology of the mitochondrion. In Trypanosoma brucei, four DNA polymerases, namely PolIA, PolIB, PolIC, and PolID, related to bacterial DNA polymerase I (PolI), were shown to be localized in mitochondria experimentally. These mitochondrion-localized DNA polymerases are phylogenetically distinct from other family A DNA polymerases, such as bacterial PolI, DNA polymerase gamma (Polγ) in human and yeasts, “plant and protist organellar DNA polymerase (POP)” in diverse eukaryotes. However, the diversity of mitochondrion-localized DNA polymerases in Euglenozoa other than Trypanosomatida is poorly understood. In this study, we discovered putative mitochondrion-localized DNA polymerases in broad members of three major classes of Euglenozoa—Kinetoplastea, Diplonemea, and Euglenida—to explore the origin and evolution of trypanosomatid PolIA-D. We unveiled distinct inventories of mitochondrion-localized DNA polymerases in the three classes: (1) PolIA is ubiquitous across the three euglenozoan classes, (2) PolIB, C, and D are restricted in kinetoplastids, (3) new types of mitochondrion-localized DNA polymerases were identified in a prokinetoplastid and diplonemids, and (4) evolutionarily distinct types of POP were found in euglenids. We finally propose scenarios to explain the inventories of mitochondrion-localized DNA polymerases in Kinetoplastea, Diplonemea, and Euglenida. View Full-Text
Keywords: DNA replication; family A DNA polymerase; plant and protist organellar DNA polymerase; Trypanosomatida; Kinetoplastea; Diplonemea; Euglenida; Prokinetoplastina DNA replication; family A DNA polymerase; plant and protist organellar DNA polymerase; Trypanosomatida; Kinetoplastea; Diplonemea; Euglenida; Prokinetoplastina
Show Figures

Figure 1

MDPI and ACS Style

Harada, R.; Hirakawa, Y.; Yabuki, A.; Kashiyama, Y.; Maruyama, M.; Onuma, R.; Soukal, P.; Miyagishima, S.; Hampl, V.; Tanifuji, G.; Inagaki, Y. Inventory and Evolution of Mitochondrion-localized Family A DNA Polymerases in Euglenozoa. Pathogens 2020, 9, 257.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop