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Review

Intelligent Mechanisms of Macrophage Apoptosis Subversion by Mycobacterium

by
Abualgasim Elgaili Abdalla
1,2,*,
Hasan Ejaz
1,
Mahjoob Osman Mahjoob
2,
Ayman Ali Mohammed Alameen
1,3,
Khalid Omer Abdalla Abosalif
1,2,
Mohammed Yagoub Mohammed Elamir
1,2 and
Mohammed Alsadig Mousa
2
1
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Al Jouf 2014, Saudi Arabia
2
Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Omdurman 14415, Sudan
3
Department of Chemical Pathology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum 11081, Sudan
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(3), 218; https://doi.org/10.3390/pathogens9030218
Submission received: 25 February 2020 / Revised: 12 March 2020 / Accepted: 15 March 2020 / Published: 16 March 2020
(This article belongs to the Special Issue Mycobacterium tuberculosis Pathogenesis, Infection Prevention and Treatment)
Browse Figure
Versions Notes

Abstract

:
Macrophages are one of the first innate defense barriers and play an indispensable role in communication between innate and adaptive immune responses, leading to restricted Mycobacterium tuberculosis (Mtb) infection. The macrophages can undergo programmed cell death (apoptosis), which is a crucial step to limit the intracellular growth of bacilli by liberating them into extracellular milieu in the form of apoptotic bodies. These bodies can be taken up by the macrophages for the further degradation of bacilli or by the dendritic cells, thereby leading to the activation of T lymphocytes. However, Mtb has the ability to interplay with complex signaling networks to subvert macrophage apoptosis. Here, we describe the intelligent strategies of Mtb inhibition of macrophages apoptosis. This review provides a platform for the future study of unrevealed Mtb anti-apoptotic mechanisms and the design of therapeutic interventions.

1. Introduction

Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis (Mtb), which affects 10 million people globally. It was one of the top 10 infectious diseases in 2017, with an estimated 1.6 million deaths worldwide, which dropped to 1.5 million in 2018 [1]. Mtb is considered a sinful intercellular pathogen that is capable of surviving and replicating within the hostile microenvironment of macrophages and other cellular niches [2]. Macrophages are at the frontline of the innate defense, encounter the pathogens, and play a critical role in the containment of bacilli infection through triggering inflammation, digestion of bacilli, and inducing adaptive immune responses [3]. However, Mtb can subvert macrophage responses such as apoptosis in order to establish a persistent lifestyle [4]. Thus, understanding the underlying mechanisms behind Mtb manipulation of macrophages apoptosis is decisive for treatment interventions and TB vaccines.
Apoptosis is a highly coordinated and regulated process of the programmed cell death form in which dying cell components and Mtb are enclosed within cytoplasmic membranes and liberated from the cell called apoptotic bodies [5]. The apoptosis of macrophages is a crucial part of the innate host defense against Mtb through restricting the intracellular growth of bacilli. The Mtb infected macrophage undergoes apoptosis in order to release the Mtb into the extracellular milieu, thereby leading to the activation of dendritic cells and triggering of robust adaptive immune responses [6,7]. Nevertheless, Mtb has evolved multiple mechanisms to revoke macrophages apoptosis, which are discussed in the current review.

2. Anti-Apoptotic Determinants of Mycobacterium

Mtb has a wide variety of effector molecules that actively block macrophage apoptotic pathways. In this context, cell wall-associated glycolipids, such as lipoarabinomannan (LAM) and mannosylated LAM (ManLAM), are important virulence determinants that have been found to modulate macrophages apoptosis [8,9]. ManLAM can thwart Mtb mediating B10R macrophage apoptosis by inhibiting calcium influx and its intracellular signaling. Ablation of calcium-signaling leads to the inhibition of caspase-1 activity, alteration of mitochondrial membrane permeability, as well as induced upregulation of anti-apoptotic, namely, B-cell lymphoma 2 (Bcl2) (Table 1) [8]. LAM can also block human leukemia monocytic cell (THP-1) apoptosis by activating phosphatidylinositol 3-kinase (PI3K), which in turn activates serine/threonine kinase (Akt) that suppresses pro-apoptotic factor Bad (Table 1) [9].
Mtb tyrosine phosphatase (PtpA) is a secreted protein that plays a decisive role in the pathogenesis and survival of Mtb within macrophages via interplay with multiple host signaling pathways [10]. PtpA knockout Mtb strain induces a high level of caspase-3 expression and promotes its activity in THP-1 macrophages when compared with wild Mtb strains. PtpA can block caspase-3 activity by dephosphorylation of human glycogen synthase kinase 3 (GSK3) (Table 1) [11]. Similarly, PtpA can attenuate differentiated U937 macrophages apoptosis in response to Bacillus Calmette–Guérin (BCG) strain infections. PtpA can abolish the ubiquitin ligase of the tripartite motif-containing (TRIM) protein activity, which is required for pro-caspase-3 cleavage [12]. Noticeable, another Mtb secreted tyrosine phosphatase (MptpB) is a decisive virulence factor that can modulate macrophage responses and enhance Mtb intracellular survival. RAW264.7 cells expressing MptpB displayed much less apoptosis than the control cells when infected with BCG strains. Furthermore, interferon-gamma (IFN-γ)-stimulated RAW264.7 cells expressing MptpB show a high viability rate and low levels of cleaved caspase-3 in response to BCG infection when compared RAW264.7 cells-harboring empty vector [13]. Taken together, the anti-apoptotic mechanisms of Mtb tyrosine phosphatases are dependent on the inhibition of caspase-3 activation (Table 1).
Mtb has 11 types of serine/threonine protein kinases (PknA through PknL) that play a significant role with respect to bacterial adaptation in hostile environments, both in vivo and in vitro [14]. Among them, pknE has anti-apoptotic activity and is indispensable for Mtb survival inside the macrophage. PknE deleted Mtb strains induce higher levels of THP-1 macrophage apoptosis than the wild type strains and can suppress oxidative stress-inducing cellular apoptosis [15,16]. PknE can modulate the expression of multiple apoptotic molecules and reduce the expression of pro-apoptotic factors, including P53, Bax, and TNF-α, while increasing the expression of anti-apoptotic factor Mcl-1 (Table 1). Furthermore, pknE can promote the phosphorylation of Akt [16], which in turn suppresses the pro-apoptotic factor, i.e., Bad protein [9].
Mtb NADH-ubiquinone oxidoreductase subunit G (nuoG), a subunit of type-1 NADH dehydrogenase (NADH-1), was demonstrated to suppressed THP-1 and murine bone marrow-derived macrophages (BMDMs) apoptosis and promoted the pathogenesis of bacilli in a mice infection model [17]. NuoG knockout Mtb strains were less virulent and lost their ability to abrogate macrophage apoptosis in comparison with control strains. The ability of nuoG deleted Mtb strains to provoke macrophage apoptosis was significantly reversed upon treatment with caspase-3 and caspase-8 inhibitors, or through the ablation of TNF-α signaling. NuoG can suppress NADPH oxidase (NOX2)-inducing of ROS production leading to inhibition of TNF-α secretion (Table 1) [18]. A recent study demonstrates that the deletion of Mtb TNF-α-suppressing genes results in increased macrophage apoptosis and promotes a cell-mediated immune response [19]. Therefore, targeting nuoG might improve the immune responses and control the intracellular growth of Mtb.
Mtb nucleoside diphosphate kinase (Ndk) acts as a small GTPase inhibitor that can deactivate GTPase Rac1 and, subsequently, inhibit NOX2 assembly and ROS production. Ndk has been demonstrated to block murine macrophage apoptosis by abolishing ROS-mediated caspase-3 cleavage (Table 1) [20].
Mtb isocitrate lyase (Icl) is crucial for bacilli viability during latent infection [21]. The recombinant M. smegmatis-expressing IcI enhances survival within RAW264.7 murine macrophages and inhibits apoptosis in comparison with M. smegmatis-harboring empty vector [22]. Nevertheless, the underlying mechanisms by which IcI subverts macrophage apoptosis are yet be determined.
Mtb PE_PGRS family proteins are the most abundant cell wall anchored proteins that are implicated in bacilli adhesion, invasion, and survival within macrophages and dendritic cells [23]. PE_PGRS62, PE_PGRS41, and PE_PGRS18 contribute actively to revoke macrophage apoptosis [24,25,26].
The ectopic expression of PE_PGRS62 in M. smegmatis enhances its survival within THP-1 macrophages and reduces cell apoptosis. It has been observed that PE_PGRS62 can inhibit the endoplasmic reticulum (ER) stress response (Table 1) by downregulating C/EBP homologous protein (CHOP) and the 78-kDa glucose-regulated protein (GRP78/Bip), which are essential pro-apoptotic factors in response to stress conditions [25]. Likewise, M. smegmatis-expressing PE_PGRS41 induces much lower THP-1 macrophage apoptosis than the M. smegmatis expressing vector. PE_PGRS41 can decrease the cleavage levels of caspase-9 and caspase-3 (Table 1) [24]. PE_PGRS18 also inhibits THP-1 apoptosis. However the underlying molecular mechanisms are still unknown [26].
Mtb secreted proteins encoded by Rv3654c and Rv3655c genes express within the cytoplasm of Mtb-infected macrophages, suggesting that they may have a role in Mtb interplays with host cell signaling [27]. These proteins abrogate the extrinsic pathway-mediated U937 cells apoptosis during Mtb infection. They interact and cleave the polypyrimidine tract binding Protein-associated Splicing Factor (PSF) result in the deactivation of caspase-8 (Table 1) [27]. Similarly, Rv3033 is a secreted protein that is crucial for Mtb viability within macrophages [28]. Rv3033 has potent anti-apoptotic activity by suppressing the intrinsic apoptotic pathway. M. smegmatis expressing Rv3033 inhibits murine BMDM apoptosis compared with control strains. Rv3033 can actively block the translocation of pro-apoptotic Bax protein into mitochondria and cytochrome c into cytoplasm, thereby leading to suppress the caspase-9 activation (Table 1) [29]. Likewise, Rv3365c is another hypothetical protein that is an important Mtb anti-apoptotic effector. The deletion of Rv3365c significantly reduces the ability of Mtb to suppress U937 cells apoptosis in comparison with wild type strains. Rv3365c can revoke cellular apoptosis by interacting and inhibiting host cell membrane-bound serine protease cathepsin G and its downstream activation of caspase-1(Table 1) [30].
Mtb enhanced intracellular survival (Eis) protein is an important modulator of macrophage apoptosis, autophagy, and inflammatory cytokines production. In the context of apoptosis, Eis knockout Mtb strain significantly increases the murine BMDM when compared with wild type or complemented strains. Eis can repress JNK signaling leading to inhibition of ROS production (Table 1) [31].
Mtb stress response proteins play a significant role in bacterial resistance to the harsh milieu in both in vitro and in vivo [32,33]. Mtb SigH is a stress response regulon that controls the expression of multiple Mtb genes in response to oxidative and heat-stressors, as well as to host microenvironments [34,35]. Lower apoptosis of primary rhesus macaque bone marrow-derived macrophage (Rh-BMDM) was observed upon infection with SigH knockdown Mtb strains compared with wild Mtb strains [33]. SigH regulon can abolish cellular apoptosis through boosting prostaglandin synthetase 2 (PTGS2) expression, which in turn inhibits the P53-dependent apoptotic pathway (Table 1) [33].
Mtb Cpn60.2 (GroEL2) is a member of heat shock proteins found in the secreted form within the cytosol of macrophage [32]. Cpn60.2 can revoke THP-1 macrophage apoptosis through interacting with, and enhancing the stability of human mitochondrial Hsp70, which is (Table 1) an important anti-apoptotic factor [32] also known as mortalin [36].
Mtb acpM is a meromycolate extension acyl carrier protein involved in cell wall mycolic acid biosynthesis and a crucial drug target to eradicate multi-drugs-resistant Mtb strains (MDR) [37]. M. smegmatis expressing-acpM (Msm-acpM) can enhance the viability of BMDM in comparison with cells infected with M. smegmatis, harboring an empty vector. AcpM reduces ROS production by suppressing c-Jun N-terminal Kinase (JNK) signaling (Table 1) [38].
Mtb LpqT is a cell wall anchored lipoprotein that modulates macrophage responses by engaging with Toll-Like Receptor-2 (TLR-2) [39]. LpqT deleted M. smegmatis strains significantly induce higher levels of RAW264.7 macrophage apoptosis compared with control strains. LpqT could decrease the cleaved levels of caspase-3 by blocking the TLR-2 signaling pathway (Table 1) [39]. However, the previous study demonstrated that LpqT could increase the apoptosis of THP-1 macrophage and monocyte-derived macrophages (MDM) via agonist TLR-2 signaling [40]. Therefore, the exact role of Mtb LpqT in the regulation of macrophage apoptosis in the context of its interaction with TLR-2 remains controversial. It has been demonstrated that lipoprotein MPT83 can induce protective immunity against Mtb infection via promoting macrophage apoptosis [41].
EspR is an Mtb specific protein and a key regulatory protein of the ESX-1 secretion system and many other genes that are involved in Mtb pathogenesis [42]. Enforced expression of EspR in RAW264.7 macrophage leading to suppressed BCG mediating apoptosis. EspR can significantly decrease the cleavage of both caspase-3 and caspase-8 (Table 1) when compared with RAW264.7 harboring empty-vector. The mechanisms underlying caspase inhibition was due to EspR interfering with TLRs signaling by directly interacting with myeloid differentiated protein-88 (MyD88) [43].

3. Mycobacterium Thwarts Macrophage Apoptosis by Inducing Anti-Apoptotic Cytokines

Cytokines are inducible, soluble immune mediators responsible for orchestrating various and complex immunological processes, including immune cells crosstalk and apoptosis [44]. Mtb can differentially induce cytokines secretion to manipulate macrophage apoptosis. An earlier study showed that Mycobacterium could inhibit macrophage apoptosis by robust inducing interleukin-10 (IL-10) production, leading to the increased expression of Bcl2 and decreasing pro-apoptotic factors such as cleaved caspase-1, P53, nitric oxide (NO), and TNF-α production (Figure 1) [45]. Transgenic mice expressing human IL-10 in antigen-presenting cells (APCs) infected with M. avium displayed lower levels of macrophage apoptosis, which was accompanied by decreased TNF-α and NO production [46]. IL-10 also inhibits Mtb inducing alveolar macrophages (AM) apoptosis by abolishing TNF-α production. In this regard, IL-10 can increase the levels of B-cell lymphoma 3 protein (Bcl-3), which in turn antagonizes TNF-α production via deactivating NF-kappaB nuclear signaling [47].
IL-17A, a cytokine secreted in high amount by T helper-17 (Th17) in response to Mtb infection and actively contributes to TB pathogenesis [48]. It has been shown that the exogenous addition of IL-17A can significantly enhance BCG strains and Mtb viability within murine BMDM when compared with bacterial intracellular growth in the absence of IL-17A. This cytokine can blockade BMDM apoptosis upon BCG infection by suppression of intrinsic apoptotic pathway through interfering with nuclear translocation of P53, thereby leading to the upregulation of Bcl2 expression and downregulation of Bax expression, as well as abolishing cytochrome c released and caspase-3 activation (Figure 1) [49].
Epstein–Barr virus-induced gene 3 (EBI3), is a subunit of anti-inflammatory cytokines, namely IL-27 and IL-35. It was found that EBI3 is produced in a significant amount in CD14+ macrophages isolated from TB patients compared to cells from healthy donors. Furthermore, it has been demonstrated that EBI3 production and accumulation were markedly increased upon the challenge of murine peritoneal macrophages with Mtb, suggesting that it may contribute to the immunoevasion of macrophage responses. Mtb and BCG strains shown to induce significant apoptosis of murine EBI3 deleted macrophages in comparison with the deaths of wild type cells. EB13 abrogates the extrinsic apoptotic pathway via suppression of the cleavage of caspase-3 and caspase-8 (Figure 1) [50]. Taken together, Mtb can stimulate IL-10, IL-17A, and EBI3 secretion to frustrate macrophage apoptosis. Thus, complementary to antibodies blocking the action of these cytokines, anti-tuberculous drugs can hasten the eradication of bacilli and shortage of treatment regimens.

4. Mtb can Suppress Apoptosis by Regulating microRNAs Expression

MicroRNAs (miRs) are small noncoding RNAs that play an indispensable role in posttranscriptional regulation of genes expression that involved in various cellular biochemical pathways, including immune signaling pathways in response to pathogenic infections such as Mtb [51].
Mtb can selectively regulate miRs expression to defeat macrophages apoptosis in order to enhance its intracellular viability and growth. Consistently, miR-582-5p has been significantly more upregulated in monocytes obtained from active TB patients than the cells from healthy controls. The transfection of THP-1 monocytic cells with miR-582-5p mimics leads to the blocking of apoptosis when compared cells transfected with the negative control. MiR-582-5p can inhibit monocytes apoptosis by directly targeting forkhead box O1 (FOXO1) mRNA and suppressing its translation (Figure 1) [52].
MiR-155 expression significantly upregulated in the peripheral blood monocytes (PBMCs) obtained from patients with active TB disease than the cells from healthy control [53]. The apoptosis of CD4+ monocytes was significantly decreased in active TB patients compared with healthy control, suggesting that miR-155 may abolish cellular apoptosis. The ectopic expression of miR-155 in THP-1 macrophages leading to abrogate BCG-inducing apoptosis via downregulating FOXO3 expression (Figure 1) [53]. Furthermore, Mtb infected miR-155 knockdown murine macrophages exhibited a higher level of apoptosis when compared with wild type macrophages infected with Mtb [54]. MiR-155 can target the mRNA of SH2 domain-containing inositol 5-phosphatase 1(SHIP1), which was confirmed by increased protein levels in miR-155 deleted macrophages. Increased levels of SHIP1 can result in reduced phosphorylation of Akt, thereby leading to the enhanced activation of Bad, FOXO-1, 3, and caspase-3 (Figure 1) [54].
MiR-223 is expressed significantly more in peripheral macrophages harvested from active TB patients compared with cells from healthy controls and upregulated during the in vitro Mtb infection of human macrophages. The forced expression miR-223 in human macrophages significantly reduced the rate of apoptosis in comparison with macrophages transfected with control miR-223 mimics. Mechanistically, miR-223 blocks macrophage apoptosis by targeting FOXO3 and markedly reducing its protein expression (Figure 1) [55].
Let-7e and miR-29a are highly expressed in human monocyte-derived macrophages in response to M. avium infection. They block macrophages apoptosis by repressing the expression of caspase-3 and 7, which are direct targets for let-7e and miR-29a, respectively (Figure 1) [56].
MiR-21 expression was upregulated by NF-κB signaling in RAW264.7 murine macrophage triggered by Mtb mpt68 protein. It has been demonstrated that miR-21 could inhibit murine macrophage apoptosis by positively regulating anti-apoptotic Bcl-2 expression (Figure 1) [57]. However, recent studies have demonstrated that miR-21 enhances macrophages apoptosis in response to mycobacterium infection by negatively regulating the expression of Bcl-2 [58,59]. Therefore, the role of miR-21 in regulating macrophage apoptosis and Bcl-2 expression remains ambiguous.
MiR-20a-5p expression was found to be downregulated in CD14+ monocytes from active pulmonary TB patients, while it is pronouncedly upregulated by successful anti-TB treatment. The downregulation of miR-20a-5p expression also observed during the Mtb infection of THP-1 human macrophages. Blockade of miR-20a-5p expression can accelerate THP-1 macrophage apoptosis and promote mycobacterium survival. MiR-20a-5p ectopically expressed in THP-1 cells led to the decreased expression of pro-apoptotic gene Bim through targeting and abolishing the expression of JNK2 signaling (Figure 1) [60]. Recently, the downregulation of miR-20b-5p expression has been reported upon the Mtb infection of RAW264.7 macrophages. The overexpression of miR-20b-5p can promote bacilli survival and cell apoptosis [61]. In this regard, miR-20b-5p can increase macrophage apoptosis by targeting and suppressing the expression of the anti-apoptotic Mcl-1 gene (Figure 1) [61], which is a member of the Bcl-2 protein family.
Hsa-let-7b-5p was significantly upregulated in Mtb infected THP-1 macrophages, leading to the subversion of apoptosis. Mechanistically, hsa-let-7b-5p negatively regulates the expression of Fas by directly targeting its mRNA, which can lead to blocking pathways of caspase-3 activation (Figure 1) [62].

5. Conclusions

Macrophages are the first line of innate defense against Mtb infection, and the subversion of macrophage apoptosis is considered a hallmark of Mtb pathogenesis. Mtb is a mystery pathogen that utilizes multiple strategies to abolish apoptotic signaling pathways in order to establish persistent infection. The impairment of macrophage apoptosis leads to the enhanced intracellular survival of bacilli and compromises the cell-mediated immune response. Mtb has a broad spectrum of anti-apoptotic effector molecules that direct targeting cellular pro-apoptotic factors or block the signaling that regulates their expression. Some Mtb anti-apoptotic effectors, including PE_PGRS18 and IcI, impaired macrophage apoptosis by unrecognized mechanisms. Moreover, the role of Mycobacterium lipoprotein (LpqT) in modulating macrophage apoptosis needed to be clarified. Future studies are needed to identify unrevealed mechanisms of Mtb anti-apoptotic effectors and to develop methods able to hamper Mtb effectors’ capability to abrogate macrophage apoptosis.
Mtb can also manipulate macrophage apoptosis by selectively regulating cytokines and miRs expression. It is also important to understand how Mtb selectively regulates anti-apoptotic cytokines and miRs expression. The mechanisms by which cytokines and miRs enhance the viability of macrophages are dependent on the aberrant activity of key pro-apoptotic regulators, including P53, TNF-α, Fas, FOXO, and Bad. They promote the activity of anti-apoptotic regulators such as SHIP, Bcl-2, and Mcl-1. Taken together, these insights into the intelligent subversion mechanisms of macrophage apoptosis by Mtb elucidate promising and novel therapeutic targets to eliminate the intracellular survival of bacilli and promote an adaptive immune response.

Author Contributions

A.E.A. conceived the idea and drafted the manuscript. M.O.M., A.A.M.A., K.O.A.A., M.Y.M.E., and M.A.M. reviewed the literature and helped in the drafting and analysis of the manuscript. H.E. contributed in drafting and critical revision of the manuscript. All authors have read and agree to the published version of the manuscript.

Funding

This research received no external funding.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. World Health Organization. Global Tuberculosis Report 2019; World Health Organization: Geneva, Switzerland, 2019; Available online: https://www.who.int/tb/publications/global_report/en/ (accessed on 17 October 2019).
  2. Marakalala, M.J.; Martinez, F.O.; Plüddemann, A.; Gordon, S. Macrophage heterogeneity in the immunopathogenesis of tuberculosis. Front. Microbiol. 2018, 9, 1028. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  3. Ferraris, D.; Miggiano, R.; Rossi, F.; Rizzi, M. Mycobacterium tuberculosis molecular determinants of infection, survival strategies, and vulnerable targets. Pathogens 2018, 7, 17. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Hmama, Z.; Peña-Díaz, S.; Joseph, S.; Av-Gay, Y. Immunoevasion and immunosuppression of the macrophage by Mycobacterium tuberculosis. Immunol. Rev. 2015, 264, 220–232. [Google Scholar] [CrossRef] [PubMed]
  5. Divangahi, M.; Behar, S.M.; Remold, H. Dying to live: How the death modality of the infected macrophage affects immunity to tuberculosis. Adv. Exp. Med. Biol. 2013, 783, 103–120. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  6. Weiss, G.; Schaible, U.E. Macrophage defense mechanisms against intracellular bacteria. Immunol. Rev. 2015, 264, 182–203. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  7. Kelly, D.M.; ten Bokum, A.M.; O’Leary, S.M.; O’Sullivan, M.P.; Keane, J. Bystander macrophage apoptosis after Mycobacterium tuberculosis H37Ra infection. Infect. Immun. 2008, 76, 351–360. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  8. Rojas, M.; García, L.F.; Nigou, J.; Puzo, G.; Olivier, M. Mannosylated lipoarabinomannan antagonizes Mycobacterium tuberculosis-induced macrophage apoptosis by altering Ca+ 2-dependent cell signaling. J. Infect. Dis. 2000, 182, 240–251. [Google Scholar] [CrossRef] [Green Version]
  9. Maiti, D.; Bhattacharyya, A.; Basu, J. Lipoarabinomannan from Mycobacterium tuberculosis promotes macrophage survival by phosphorylating bad through a phosphatidylinositol 3-kinase/Akt pathway. J. Biol. Chem. 2001, 276, 329–333. [Google Scholar] [CrossRef] [Green Version]
  10. Wong, D.; Chao, J.D.; Av-Gay, Y. Mycobacterium tuberculosis-secreted phosphatases: From pathogenesis to targets for TB drug development. Trends Microbiol. 2013, 21, 100–109. [Google Scholar] [CrossRef]
  11. Poirier, V.; Bach, H.; Av-Gay, Y. Mycobacterium tuberculosis promotes anti-apoptotic activity of the macrophage by PtpA protein-dependent dephosphorylation of host GSK3α. J. Biol. Chem. 2014, 289, 29376–29385. [Google Scholar] [CrossRef] [Green Version]
  12. Wang, J.; Teng, J.L.; Zhao, D. The ubiquitin ligase TRIM27 functions as a host restriction factor antagonized by Mycobacterium tuberculosis PtpA during mycobacterial infection. Sci. Rep. 2016, 6, 34827. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Fan, L.; Wu, X.; Jin, C.; Li, F.; Xiong, S.; Dong, Y. MptpB promotes Mycobacteria survival by inhibiting the expression of inflammatory mediators and cell apoptosis in macrophages. Front. Cell Infect. Microbiol. 2018, 8, 171. [Google Scholar] [CrossRef] [PubMed]
  14. Prisic, S.; Husson, R.N. Mycobacterium tuberculosis serine/threonine protein kinases. Microbiol. Spectr. 2014, 2, 1–42. [Google Scholar] [CrossRef] [Green Version]
  15. Jayakumar, D.; Jacobs, W.R.; Narayanan, S. Protein kinase E of Mycobacterium tuberculosis has a role in the nitric oxide stress response and apoptosis in a human macrophage model of infection. Cell Microbiol. 2008, 10, 365–374. [Google Scholar] [CrossRef] [Green Version]
  16. Kumar, D.; Narayanan, S. pknE, a serine/threonine kinase of Mycobacterium tuberculosis modulates multiple apoptotic paradigms. Infect. Genet. Evol. 2012, 12, 737–747. [Google Scholar] [CrossRef]
  17. Velmurugan, K.; Chen, B.; Miller, J.L. Mycobacterium tuberculosis nuoG is a virulence gene that inhibits apoptosis of infected host cells. PLoS Pathog. 2007, 3, e110. [Google Scholar] [CrossRef] [Green Version]
  18. Miller, J.L.; Velmurugan, K.; Cowan, M.J.; Briken, V. The type I NADH dehydrogenase of Mycobacterium tuberculosis counters phagosomal NOX2 activity to inhibit TNF-α-mediated host cell apoptosis. PLoS Pathog. 2010, 6, e1000864. [Google Scholar] [CrossRef]
  19. Olsen, A.; Chen, Y.; Ji, Q.; Zhu, G.; De Silva, A.D.; Vilchèze, C.; Weisbrod, T.; Li, W.; Xu, J.; Larsen, M.; et al. Targeting Mycobacterium tuberculosis tumor necrosis factor alpha-downregulating genes for the development of antituberculous vaccines. MBio 2016, 7, e01023-15. [Google Scholar] [CrossRef] [Green Version]
  20. Sun, J.; Singh, V.; Lau, A.; Stokes, R.W.; Obregón-Henao, A.; Orme, I.M.; Wong, D.; Av-Gay, Y.; Hmama, Z. Mycobacterium tuberculosis nucleoside diphosphate kinase inactivates small GTPases leading to evasion of innate immunity. PLoS Pathog. 2013, 9, e1003499. [Google Scholar] [CrossRef] [Green Version]
  21. Bhusal, R.P.; Bashiri, G.; Kwai, B.X.; Sperry, J.; Leung, I.K. Targeting isocitrate lyase for the treatment of latent tuberculosis. Drug Discov. Today 2017, 22, 1008–1016. [Google Scholar] [CrossRef]
  22. Li, J.M.; Na, L.; Zhu, D.Y.; Wan, L.G.; He, Y.L.; Chun, Y. Isocitrate lyase from Mycobacterium tuberculosis promotes survival of Mycobacterium smegmatis within macrophage by suppressing cell apoptosis. Chin. Med. J. 2008, 121, 1114–1119. [Google Scholar] [CrossRef] [PubMed]
  23. Meena, L.S. An overview to understand the role of PE_PGRS family proteins in Mycobacterium tuberculosis H37Rv and their potential as new drug targets. Biotechnol. Appl. Biochem. 2015, 62, 145–153. [Google Scholar] [CrossRef] [PubMed]
  24. Deng, W.; Long, Q.; Zeng, J.; Li, P.; Yang, W.; Chen, X.; Xie, J. Mycobacterium tuberculosis PE_PGRS41 enhances the intracellular survival of M. smegmatis within macrophages via blocking innate immunity and inhibition of host defense. Sci. Rep. 2017, 7, 46716. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Long, Q.; Xiang, X.; Yin, Q.; Li, S.; Yang, W.; Sun, H.; Xie, J.; Deng, W. PE_PGRS62 promotes the survival of Mycobacterium smegmatis within macrophages via disrupting ER stress-mediated apoptosis. J. Cell. Physiol. 2019, 234, 19774–19784. [Google Scholar] [CrossRef] [PubMed]
  26. Yang, W.; Deng, W.; Zeng, J.; Ren, S.; Ali, M.K.; Gu, Y.; Li, Y.; Xie, J. Mycobacterium tuberculosis PE_PGRS18 enhances the intracellular survival of M. smegmatis via altering host macrophage cytokine profiling and attenuating the cell apoptosis. Apoptosis 2017, 22, 502–509. [Google Scholar] [CrossRef]
  27. Danelishvili, L.; Yamazaki, Y.; Selker, J.; Bermudez, L.E. Secreted Mycobacterium tuberculosis Rv3654c and Rv3655c proteins participate in the suppression of macrophage apoptosis. PLoS ONE 2010, 5, e10474. [Google Scholar] [CrossRef]
  28. Rengarajan, J.; Bloom, B.R.; Rubin, E.J. Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages. Proc. Natl. Acad. Sci. USA 2005, 102, 8327–8332. [Google Scholar] [CrossRef] [Green Version]
  29. Zhang, W.; Lu, Q.; Dong, Y.; Yue, Y.; Xiong, S. Rv3033, as an Emerging Anti-apoptosis Factor, Facilitates Mycobacteria Survival via Inhibiting Macrophage Intrinsic Apoptosis. Front. Immunol. 2018, 9, 2136. [Google Scholar] [CrossRef] [Green Version]
  30. Danelishvili, L.; Everman, J.; McNamara, M.; Bermudez, L. Inhibition of the plasma-membrane-associated serine protease cathepsin G by Mycobacterium tuberculosis Rv3364c suppresses caspase-1 and pyroptosis in macrophages. Front. Microbiol. 2012, 2, 281. [Google Scholar] [CrossRef] [Green Version]
  31. Shin, D.M.; Jeon, B.Y.; Lee, H.M.; Jin, H.S.; Yuk, J.M.; Song, C.H.; Lee, S.H.; Lee, Z.W.; Cho, S.N.; Kim, J.M.; et al. Mycobacterium tuberculosis eis regulates autophagy, inflammation, and cell death through redox-dependent signaling. PLoS Pathog. 2010, 6, e1001230. [Google Scholar] [CrossRef] [Green Version]
  32. Joseph, S.; Yuen, A.; Singh, V.; Hmama, Z. Mycobacterium tuberculosis Cpn60. 2 (GroEL2) blocks macrophage apoptosis via interaction with mitochondrial mortalin. Biol. Open 2017, 6, 481–488. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Dutta, N.K.; Mehra, S.; Martinez, A.N.; Alvarez, X.; Renner, N.A.; Morici, L.A.; Pahar, B.; Maclean, A.G.; Lackner, A.A.; Kaushal, D. The stress-response factor SigH modulates the interaction between Mycobacterium tuberculosis and host phagocytes. PLoS ONE 2012, 7, e28958. [Google Scholar] [CrossRef]
  34. Raman, S.; Song, T.; Puyang, X.; Bardarov, S.; Jacobs, W.R.; Husson, R.N. The alternative sigma factor SigH regulates major components of oxidative and heat stress responses in Mycobacterium tuberculosis. J. Bacteriol. 2001, 183, 6119–6125. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Mehra, S.; Golden, N.A.; Stuckey, K.; Didier, P.J.; Doyle, L.A.; Russell-Lodrigue, K.E.; Sugimoto, C.; Hasegawa, A.; Sivasubramani, S.K.; Roy, C.J.; et al. The Mycobacterium tuberculosis stress response factor SigH is required for bacterial burden as well as immunopathology in primate lungs. J. Infect. Dis. 2012, 205, 1203–1213. [Google Scholar] [CrossRef] [PubMed]
  36. Londono, C.; Osorio, C.; Gama, V.; Alzate, O. Mortalin, apoptosis, and neurodegeneration. Biomolecules 2012, 2, 143–164. [Google Scholar] [CrossRef] [Green Version]
  37. Kaur, D.; Mathew, S.; Nair, C.; Begum, A.; Jainanarayan, A.K.; Sharma, M.; Brahmachari, S.K. Structure based drug discovery for designing leads for the non-toxic metabolic targets in multi drug resistant Mycobacterium tuberculosis. J. Transl. Med. 2017, 15, 261. [Google Scholar] [CrossRef] [Green Version]
  38. Paik, S.; Choi, S.; Lee, K.I.; Back, Y.W.; Son, Y.J.; Jo, E.K.; Kim, H.J. Mycobacterium tuberculosis acyl carrier protein inhibits macrophage apoptotic death by modulating the reactive oxygen species/c-Jun N-terminal kinase pathway. Microbes Infect. 2019, 21, 40–49. [Google Scholar] [CrossRef]
  39. Li, F.; Feng, L.; Jin, C.; Wu, X.; Fan, L.; Xiong, S.; Dong, Y. LpqT improves mycobacteria survival in macrophages by inhibiting TLR2 mediated inflammatory cytokine expression and cell apoptosis. Tuberculosis 2018, 111, 57–66. [Google Scholar] [CrossRef]
  40. Su, H.; Zhu, S.; Zhu, L.; Huang, W.; Wang, H.; Zhang, Z.; Xu, Y. Recombinant lipoprotein Rv1016c derived from Mycobacterium tuberculosis is a TLR-2 ligand that induces macrophages apoptosis and inhibits MHC II antigen processing. Front Cell. Infect. Microbiol. 2016, 6, 147. [Google Scholar] [CrossRef] [Green Version]
  41. Blasco, B.; Chen, J.M.; Hartkoorn, R.; Sala, C.; Uplekar, S.; Rougemont, J.; Pojer, F.; Cole, S.T. Virulence regulator EspR of Mycobacterium tuberculosis is a nucleoid-associated protein. PLoS Pathog. 2012, 8, e1002621. [Google Scholar] [CrossRef] [Green Version]
  42. Wang, L.; Zuo, M.; Chen, H.; Liu, S.; Wu, X.; Cui, Z.; Yang, H.; Liu, H.; Ge, B. Mycobacterium tuberculosis lipoprotein MPT83 induces apoptosis of infected macrophages by activating the TLR2/p38/COX-2 signaling pathway. J. Immunol. 2017, 198, 4772–4780. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Jin, C.; Wu, X.; Dong, C.; Li, F.; Fan, L.; Xiong, S.; Dong, Y. EspR promotes mycobacteria survival in macrophages by inhibiting MyD88 mediated inflammation and apoptosis. Tuberculosis 2019, 116, 22–31. [Google Scholar] [CrossRef] [PubMed]
  44. Etna, M.P.; Giacomini, E.; Severa, M.; Coccia, E.M. Pro-and anti-inflammatory cytokines in tuberculosis: A two-edged sword in TB pathogenesis. Semin. Immunol. 2014, 26, 543–551. [Google Scholar] [CrossRef] [PubMed]
  45. Balcewicz-Sablinska, M.K.; Gan, H.; Remold, H. Interleukin 10 produced by macrophages inoculated with Mycobacterium avium attenuates mycobacteria-induced apoptosis by reduction of TNF-α activity. J. Infect Dis. 1999, 180, 1230–1237. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  46. Feng, C.G.; Kullberg, M.C.; Jankovic, D.; Cheever, A.W.; Caspar, P.; Coffman, R.L.; Sher, A. Transgenic mice expressing human interleukin-10 in the antigen-presenting cell compartment show increased susceptibility to infection with Mycobacterium avium associated with decreased macrophage effector function and apoptosis. Infect. Immun. 2002, 70, 6672–6679. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  47. Patel, N.R.; Swan, K.; Li, X.; Tachado, S.D.; Koziel, H. Impaired M. tuberculosis—Mediated apoptosis in alveolar macrophages from HIV+ persons: Potential role of IL-10 and BCL-3. J. Leukoc. Biol. 2009, 86, 53–60. [Google Scholar] [CrossRef] [Green Version]
  48. Pollara, G.; Turner, C.T.; Tomlinson, G.S.; Bell, L.C.; Khan, A.; Peralta, L.F.; Ricciardolo, F.L. Exaggerated in vivo IL-17 responses discriminate recall responses in active TB. bioRxiv 2019. bioRxiv: 516690. [Google Scholar]
  49. Cruz, A.; Ludovico, P.; Torrado, E.; Gama, J.B.; Sousa, J.; Gaifem, J.; Appelberg, R.; Rodrigues, F.; Cooper, A.M.; Pedrosa, J.; et al. IL-17A promotes intracellular growth of Mycobacterium by inhibiting apoptosis of infected macrophages. Front. Immunol. 2015, 6, 498. [Google Scholar] [CrossRef] [Green Version]
  50. Deng, J.H.; Chen, H.Y.; Huang, C.; Yan, J.M.; Yin, Z.; Zhang, X.L.; Pan, Q. Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages. Pathog. Dis. 2019, 77, ftz007. [Google Scholar] [CrossRef] [Green Version]
  51. Zhou, X.; Li, X.; Wu, M. miRNAs reshape immunity and inflammatory responses in bacterial infection. Signal Transduct. Target. Ther. 2018, 3, 14. [Google Scholar] [CrossRef] [Green Version]
  52. Liu, Y.; Jiang, J.; Wang, X.; Zhai, F.; Cheng, X. miR-582-5p is upregulated in patients with active tuberculosis and inhibits apoptosis of monocytes by targeting FOXO1. PLoS ONE 2013, 8, e78381. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Huang, J.; Jiao, J.; Xu, W.; Zhao, H.; Zhang, C.; Shi, Y.; Xiao, Z. MiR-155 is upregulated in patients with active tuberculosis and inhibits apoptosis of monocytes by targeting FOXO3. Mol. Med. Rep. 2015, 12, 7102–7108. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  54. Rothchild, A.C.; Sissons, J.R.; Shafiani, S.; Plaisier, C.; Min, D.; Mai, D.; Gilchrist, M.; Peschon, J.; Larson, R.P.; Bergthaler, A.; et al. MiR-155–regulated molecular network orchestrates cell fate in the innate and adaptive immune response to Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 2016, 113, E6172–E6181. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  55. Xi, X.; Zhang, C.; Han, W.; Zhao, H.; Zhang, H.; Jiao, J. MicroRNA-223 is upregulated in active tuberculosis patients and inhibits apoptosis of macrophages by targeting FOXO3. Genet. Test. Mol. Biomark. 2015, 19, 650–656. [Google Scholar] [CrossRef]
  56. Sharbati, J.; Lewin, A.; Kutz-Lohroff, B.; Kamal, E.; Einspanier, R.; Sharbati, S. Integrated microRNA-mRNA-analysis of human monocyte derived macrophages upon Mycobacterium avium subsp. hominissuis infection. PLoS ONE 2011, 6, e20258. [Google Scholar] [CrossRef]
  57. Wang, Q.; Liu, S.; Tang, Y.; Liu, Q.; Yao, Y. MPT64 protein from Mycobacterium tuberculosis inhibits apoptosis of macrophages through NF-kB-miRNA21-Bcl-2 pathway. PLoS ONE 2014, 9, e100949. [Google Scholar] [CrossRef]
  58. Xue, X.; Qiu, Y.; Yang, H.L. Immunoregulatory role of MicroRNA-21 in macrophages in response to bacillus calmette-guerin infection involves modulation of the TLR4/MyD88 signaling pathway. Cell. Physiol. Biochem. 2017, 42, 91–102. [Google Scholar] [CrossRef]
  59. Zhao, Z.; Hao, J.; Li, X.; Chen, Y.; Qi, X. MiR-21-5p regulates mycobacterial survival and inflammatory responses by targeting Bcl-2 and TLR4 in Mycobacterium tuberculosis-infected macrophages. FEBS Lett. 2019, 593, 1326–1335. [Google Scholar] [CrossRef]
  60. Zhang, G.; Liu, X.; Wang, W.; Cai, Y.; Li, S.; Chen, Q.; Liao, M.; Zhang, M.; Zeng, G.; Zhou, B.; et al. Down-regulation of miR-20a-5p triggers cell apoptosis to facilitate mycobacterial clearance through targeting JNK2 in human macrophages. Cell Cycle 2016, 15, 2527–2538. [Google Scholar] [CrossRef] [Green Version]
  61. Zhang, D.; Yi, Z.; Fu, Y. Downregulation of miR-20b-5p facilitates Mycobacterium tuberculosis survival in RAW 264.7 macrophages via attenuating the cell apoptosis by Mcl-1 upregulation. J. Cell. Biochem. 2019, 120, 5889–5896. [Google Scholar] [CrossRef]
  62. Tripathi, A.; Srivastava, V.; Singh, B.N. hsa-let-7b-5p facilitates Mycobacterium tuberculosis survival in THP-1 human macrophages by Fas downregulation. FEMS Microbiol. Lett. 2018, 365, fny040. [Google Scholar] [CrossRef] [PubMed]
Pathogens 09 00218 g001 550
Figure 1. Mycobacterium evades macrophage apoptosis by induction of anti-apoptotic cytokines and miRNAs expression. Three cytokines, namely, IL-10, IL-17A, and EBI3, were demonstrated to play an anti-apoptotic role during Mtb infection. IL-10 can mediate the expression of anti-apoptotic Bcl2 and Bcl3 protein and suppresses the production of pro-apoptotic factors, including TNF-α, nitric oxide (NO), and P53. IL-17A signaling leads to suppression of P53 mediated Bax mitochondrial translocation. EBI3 can directly inhibit caspase-8 and caspase-3 activation. Nine anti-apoptotic miRs were found selectively regulated by Mtb. Has-let-7b-5p can target Fas leading to inhibition of downstream activation of caspase-3. Let-7a and miR-29a are targeting caspase-3 and caspase-7, respectively. MiR-155 can abolish expression of SH2 domain-containing inositol 5-phosphatase 1(SHIP1), leading to activation of phosphatidylinositol 3-kinase (PI3K) signaling-mediated inhibition of pro-apoptotic factors including Bad, FOXO-1 and 3. In addition, miR-155 can directly targeting and suppressing the expression of FOXO-3. MiR-582-5p can target the mRNA of FOXO-1 and block its expression. MiR-20a-5p can abrogate the activation of pro-apoptotic factor Bim by targeting the JNK-2 signaling pathway. However, miR-20b-5p can enhance the expression of anti-apoptotic factor Mcl-1, which can mediate block Bax mitochondrial translocation. The role of miR-21 in regulating anti-apoptotic Bcl-2 remains controversial.
Figure 1. Mycobacterium evades macrophage apoptosis by induction of anti-apoptotic cytokines and miRNAs expression. Three cytokines, namely, IL-10, IL-17A, and EBI3, were demonstrated to play an anti-apoptotic role during Mtb infection. IL-10 can mediate the expression of anti-apoptotic Bcl2 and Bcl3 protein and suppresses the production of pro-apoptotic factors, including TNF-α, nitric oxide (NO), and P53. IL-17A signaling leads to suppression of P53 mediated Bax mitochondrial translocation. EBI3 can directly inhibit caspase-8 and caspase-3 activation. Nine anti-apoptotic miRs were found selectively regulated by Mtb. Has-let-7b-5p can target Fas leading to inhibition of downstream activation of caspase-3. Let-7a and miR-29a are targeting caspase-3 and caspase-7, respectively. MiR-155 can abolish expression of SH2 domain-containing inositol 5-phosphatase 1(SHIP1), leading to activation of phosphatidylinositol 3-kinase (PI3K) signaling-mediated inhibition of pro-apoptotic factors including Bad, FOXO-1 and 3. In addition, miR-155 can directly targeting and suppressing the expression of FOXO-3. MiR-582-5p can target the mRNA of FOXO-1 and block its expression. MiR-20a-5p can abrogate the activation of pro-apoptotic factor Bim by targeting the JNK-2 signaling pathway. However, miR-20b-5p can enhance the expression of anti-apoptotic factor Mcl-1, which can mediate block Bax mitochondrial translocation. The role of miR-21 in regulating anti-apoptotic Bcl-2 remains controversial.
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Table
Table 1. Anti-apoptotic effectors of Mycobacterium.
Table 1. Anti-apoptotic effectors of Mycobacterium.
EffectorCell ModelMechanismsOutcomeReferences
ManLAMB10RBlocks Ca+2 influx to the cells.Inhibition of caspase-1 cleavage, alter mitochondrial membrane permeability and upregulate Bcl-2Rojas et al., 2000
LAMTHP-1Activation of PI3K signaling Suppression of BadMaiti et al., 2001
PtpATHP-1Dephosphorylation of GSK3Inhibition of caspase-3 cleavage Poirier et al., 2014
U937Suppress ubiquitin ligase activity of the TRIM protein Inhibition of caspase-3 cleavageWang et al., 2016
MptpARAW264.7Reduction of P53 levelsInhibition of caspase-3 cleavageFan et al., 2018
PknETHP-1Phosphorylation of Akt Inhibition of BadKumar and Narayanan et al., 2012
Inhibit the expression of pro-apoptotic factors, including P53, TNF-α and BaxInhibition of caspase-3 activation
Promote the anti-apoptotic factor Mcl-1 expression Block Bax mitochondrial translocation
NuoGTHP-1 and BMDMBlocks of NADPH oxidase mediating ROS productionInhibition of TNF-α production Miller et al., 2010
Ndk RAW264.7Inhibit NOX2 assembly and ROS productionInhibition of caspase-3 activationSun et al., 2013
IclRAW264.7Unknown Unknown Li et al., 2008
PE_PGRS62THP-1Suppression of pro-apoptotic stress-response genes expressions such as CHOP and GRP78/BipInhibit endoplasmic reticulum (ER) stress responseLong et al., 2019
PE_PGRS41THP-1Uncertain Reduction the cleavage level of caspase 3 and 9Deng et al., 2017
PE_PGRS18THP-1Unknown UnknownYang et al., 2017
Rv3654c
Rv3655c		U937Degrade the polypyrimidine tract binding PSFSuppression of caspase-8 activationDanelishvili et al., 2010
Rv3033RAW264.7 and murine BMDMAbolish translocation of Bax into mitochondria and cytochrome c into cytoplasmSuppression of caspase-9 activationZhang et al., 2018
Rv3365cU937Inhibit serine cathepsin GSuppression of caspase-1Danelishvili et al., 2012
Eis Murine BMDMBlock the JNK signaling Inhibition of ROS productionShin et al., 2010
SigHRh-BMDMPromote prostaglandin synthetase-2 expressionInhibition P53 dependent pathwayDutta et al., 2012
AcpMMurine BMDM Suppress JNK signalingReduction of ROS productionPaik et al., 2019
LpqTRAW264.7Antagonized TLR-2 signaling Inhibition of caspase-3 cleavage Li et al., 2018
EspRRAW264.7Block TLR signaling Inhibition of caspase-8 and 3 cleavageJin et al., 2019
PI3K, phosphatidylinositol 3-kinase; GSK3, glycogen synthetase-3; TRIM, tripartite motif; CHOP, C/EBP homologous protein; GRP78/Bip, 78-kDa glucose-regulated protein; PSF, Protein-associated Splicing Factor; TLR, Toll-Like Receptor; JNK, c-Jun N-terminal Kinase; BMDM, bone marrow-derived macrophages; Rh, Rhesus.

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Abdalla, A.E.; Ejaz, H.; Mahjoob, M.O.; Alameen, A.A.M.; Abosalif, K.O.A.; Elamir, M.Y.M.; Mousa, M.A. Intelligent Mechanisms of Macrophage Apoptosis Subversion by Mycobacterium. Pathogens 2020, 9, 218. https://doi.org/10.3390/pathogens9030218

AMA Style

Abdalla AE, Ejaz H, Mahjoob MO, Alameen AAM, Abosalif KOA, Elamir MYM, Mousa MA. Intelligent Mechanisms of Macrophage Apoptosis Subversion by Mycobacterium. Pathogens. 2020; 9(3):218. https://doi.org/10.3390/pathogens9030218

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Abdalla, Abualgasim Elgaili, Hasan Ejaz, Mahjoob Osman Mahjoob, Ayman Ali Mohammed Alameen, Khalid Omer Abdalla Abosalif, Mohammed Yagoub Mohammed Elamir, and Mohammed Alsadig Mousa. 2020. "Intelligent Mechanisms of Macrophage Apoptosis Subversion by Mycobacterium" Pathogens 9, no. 3: 218. https://doi.org/10.3390/pathogens9030218

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