Next Article in Journal
Detection of Helicobacter pylori Microevolution and Multiple Infection from Gastric Biopsies by Housekeeping Gene Amplicon Sequencing
Previous Article in Journal
Characterization of Salmonella enterica Isolates from Diseased Poultry in Northern China between 2014 and 2018
Open AccessArticle

Map3k14 as a Regulator of Innate and Adaptive Immune Response during Acute Viral Infection

1
Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Hufelandstraße 55, 45147 Essen, Germany
2
Department of Molecular Medicine II, Medical Faculty, Heinrich Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany
3
Immunology in Cancer and Infection Laboratory, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia
*
Authors to whom correspondence should be addressed.
These authors jointly supervised this work.
Pathogens 2020, 9(2), 96; https://doi.org/10.3390/pathogens9020096
Received: 6 December 2019 / Revised: 21 January 2020 / Accepted: 31 January 2020 / Published: 4 February 2020
(This article belongs to the Section Immunological Responses and Immune Defense Mechanisms)
The replication of virus in secondary lymphoid organs is crucial for the activation of antigen-presenting cells. Balanced viral replication ensures the sufficient availability of antigens and production of cytokines, and both of which are needed for virus-specific immune activation and viral elimination. Host factors that regulate coordinated viral replication are not fully understood. In the study reported here, we identified Map3k14 as an important regulator of enforced viral replication in the spleen while performing genome-wide association studies of various inbred mouse lines in a model of lymphocytic choriomeningitis virus (LCMV) infection. When alymphoplasia mice (aly/aly, Map3k14aly/aly, or Nikaly/aly), which carry a mutation in Map3k14, were infected with LCMV or vesicular stomatitis virus (VSV), they display early reductions in early viral replication in the spleen, reduced innate and adaptive immune activation, and lack of viral control. Histologically, scant B cells and the lack of CD169+ macrophages correlated with reduced immune activation in Map3k14aly/aly mice. The transfer of wildtype B cells into Map3k14aly/aly mice repopulated CD169+ macrophages, restored enforced viral replication, and resulted in enhanced immune activation and faster viral control. View Full-Text
Keywords: viral infection; lymphocytic choriomeningitis virus; vesicular stomatitis virus; genome-wide association study; Alymphoplasia mice; marginal zone viral infection; lymphocytic choriomeningitis virus; vesicular stomatitis virus; genome-wide association study; Alymphoplasia mice; marginal zone
Show Figures

Figure 1

MDPI and ACS Style

Hamdan, T.A.; Bhat, H.; Cham, L.B.; Adomati, T.; Lang, J.; Li, F.; Murtaza, A.; Hardt, C.; Lang, P.A.; Duhan, V.; Lang, K.S. Map3k14 as a Regulator of Innate and Adaptive Immune Response during Acute Viral Infection. Pathogens 2020, 9, 96.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop