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Open AccessArticle

Characterization of Salmonella enterica Isolates from Diseased Poultry in Northern China between 2014 and 2018

1
Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
2
College of Food and Pharmaceutical Engineering, Guiyang University, Guiyang City 550002, Guizhou Province, China
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(2), 95; https://doi.org/10.3390/pathogens9020095
Received: 16 January 2020 / Revised: 26 January 2020 / Accepted: 29 January 2020 / Published: 4 February 2020
(This article belongs to the Section Waterborne/Foodborne/Airborne Pathogens)
Salmonella infection not only causes acute and chronic diseases in poultry flocks, but the infected poultry are among the most important reservoirs for a variety of Salmonella serovars frequently transmitted to humans. This study aimed to investigate the occurrence of Salmonella spp. in local poultry farms in China. Samples (n = 4255), including dead-in-shell embryos, culled day-old-hatchings and 1- to 4-week-old diseased birds, were collected for Salmonella culture from broiler chicken, meat-type duck and pigeon farms in northern China between 2014 and 2018. A total of 103 Salmonella were isolated. S. enterica serovar Enteritidis and S. Typhimurium were the most prevalent serovars, representing 53.4% and 34.9% of the isolates, respectively. Serovar diversity was the highest in ducks, with the S. Apeyeme being isolated for the first time from duck tissues. All isolates were characterized by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). MLST showed that all S. Enteritidis isolates shared the same sequence type (ST11), and Typhimurium showed several rare STs in addition to ST19. In comparison, PFGE showed better discrimination for S. Enteritidis and S. Typhimurium isolates, with nine distinct pulsotypes being observed. The isolates exhibited varying degrees of resistance to 15 tested antimicrobials and identified S. Enteritidis isolates (98.18%) with multiple antimicrobial resistance were a cause for concern. Our data on invasive Salmonella infection in meat-type poultry in local farms can be used to identify sources and factors associated with Salmonella spread in poultry and the associated food chain. View Full-Text
Keywords: Salmonella; duck; chicken; pigeon; MLST; PFGE; antimicrobial resistance Salmonella; duck; chicken; pigeon; MLST; PFGE; antimicrobial resistance
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MDPI and ACS Style

Wang, J.; Li, J.; Liu, F.; Cheng, Y.; Su, J. Characterization of Salmonella enterica Isolates from Diseased Poultry in Northern China between 2014 and 2018. Pathogens 2020, 9, 95.

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