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Chrysin-Loaded Chitosan Nanoparticles Potentiates Antibiofilm Activity against Staphylococcus aureus

Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605014, India
Nanosciences African network (NANOAFNET), Materials Research Group (MRG), iThemba LABS-National Research Foundation (NRF), Old Faure Road, 7129, P O Box 722, Somerset West, Western Cape Province, South Africa
Department of Biomedical & Pharmaceutical Sciences, Chapman University, School of Pharmacy, Irvine, CA 92618-1908, USA
Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia
Authors to whom correspondence should be addressed.
Pathogens 2020, 9(2), 115;
Received: 26 December 2019 / Revised: 27 January 2020 / Accepted: 6 February 2020 / Published: 12 February 2020
The application of nanotechnology in medicine is gaining popularity due to its ability to increase the bioavailability and biosorption of numerous drugs. Chrysin, a flavone constituent of Orocylumineicum vent is well-reported for its biological properties. However, its therapeutic potential has not been fully exploited due to its poor solubility and bioavailability. In the present study, chrysin was encapsulated into chitosan nanoparticles using TPP as a linker. The nanoparticles were characterized and investigated for their anti-biofilm activity against Staphylococcus aureus. At sub-Minimum Inhibitory Concentration, the nanoparticles exhibited enhanced anti-biofilm efficacy against S. aureus as compared to its bulk counterparts, chrysin and chitosan. The decrease in the cell surface hydrophobicity and exopolysaccharide production indicated the inhibitory effect of the nanoparticles on the initial stages of biofilm development. The growth curve analysis revealed that at a sub-MIC, the nanoparticles did not exert a bactericidal effect against S. aureus. The findings indicated the anti-biofilm activity of the chrysin-loaded chitosan nanoparticles and their potential application in combating infections associated with S. aureus.
Keywords: biofilm; chitosan; chrysin; nanoparticles; Staphylococcus aureus biofilm; chitosan; chrysin; nanoparticles; Staphylococcus aureus
MDPI and ACS Style

Siddhardha, B.; Pandey, U.; Kaviyarasu, K.; Pala, R.; Syed, A.; Bahkali, A.H.; Elgorban, A.M. Chrysin-Loaded Chitosan Nanoparticles Potentiates Antibiofilm Activity against Staphylococcus aureus. Pathogens 2020, 9, 115.

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